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AB315073

Human LAMA1 ELISA Kit

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Human LAMA1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human LAMA1 with a sensitivity of 5.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

LAMA, LAMA1, Laminin subunit alpha-1, Laminin A chain, Laminin-1 subunit alpha, Laminin-3 subunit alpha, S-laminin subunit alpha, S-LAM alpha

2 Images
Sandwich ELISA - Human LAMA1 ELISA Kit (AB315073)
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Supplier Data

Sandwich ELISA - Human LAMA1 ELISA Kit (AB315073)

Example of Human LAMA1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human LAMA1 ELISA Kit (AB315073)
  • sELISA

Supplier Data

Sandwich ELISA - Human LAMA1 ELISA Kit (AB315073)

Interpolated concentration of native LAMA1 was measured in duplicate at different sample concentrations. Undiluted samples are 1.0 mg/mL HEK-293 cell extract and 1.0 mg/mL human testis tissue extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 5.7 pg/mL

Range

34.375 - 2200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human LAMA1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LAMA1 protein in Cell Lysate, Tissue extracts. Quantitate Human LAMA1 with 5.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.39" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "82 - 88 %", "average": "= 86" }, { "sample": "Tissue Extracts", "range": "97 - 111 %", "average": "= 104" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAMA1 also known as laminin subunit alpha-1 is a component of the extracellular matrix with a molecular mass of approximately 400 kDa. It is part of the laminin family a group of glycoproteins that play key roles in maintaining structural integrity and signaling in tissues. LAMA1 is widely expressed in basement membranes of many tissues particularly in the brain kidney and other organs with complex architecture. Researchers often explore its expression patterns using techniques like laminin histology and laminin microscope analysis.
Biological function summary

This protein acts as a structural scaffold and cell adhesion mediator. LAMA1 integrates into the laminin-1 trimer complex composed of alpha beta and gamma chains supporting cellular attachment differentiation and migration. This assembly aids in tissue morphogenesis and repair processes. It creates an important interface between cells and the extracellular matrix influencing cellular behavior and maintaining tissue function.

Pathways

LAMA1 interacts with cellular signaling cascades such as the PI3K/AKT pathway and the TGF-beta pathway. These pathways play significant roles in cellular growth survival and differentiation. LAMA1's interaction with other proteins like integrins and heparan sulfate proteoglycans facilitates its role in these pathways impacting diverse processes like embryonic development and tissue remodeling.

LAMA1 shows a connection to conditions such as Pierson syndrome and certain congenital muscular dystrophies. Mutations in LAMA1 can lead to basement membrane defects contributing to these diseases' pathological features. Additionally interactions with proteins like collagen IV and perlecan are significant as these complexes can further influence disease severity and progression. Understanding LAMA1's role provides insights into potential therapeutic approaches for related disorders.
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Product protocols

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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
See full target information LAMA1
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB315276

Anti-LAMA1 antibody [EPR27259-172] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [EPR27259-172] - BSA and Azide free (AB315276)

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