AB282301

Human LAMC2 ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Human LAMC2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human LAMC2 with a sensitivity of 2.16 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

LAMB2T, LAMNB2, LAMC2, Laminin subunit gamma-2, Cell-scattering factor 140 kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Kalinin/nicein/epiligrin 100 kDa subunit, Ladsin 140 kDa subunit, Laminin B2t chain, Laminin-5 subunit gamma, Large adhesive scatter factor 140 kDa subunit, Nicein subunit gamma, CSF 140 kDa subunit

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Sandwich ELISA - Human LAMC2 ELISA Kit (AB282301)

sELISA

Supplier Data

Sandwich ELISA - Human LAMC2 ELISA Kit (AB282301)

Interpolated concentrations of native LAMC2 in human cell culture supernatant samples.

The concentrations of LAMC2 were measured in duplicates, interpolated from the LAMC2 standard curves and corrected for sample dilution. Undiluted samples are as follows : A431 supernatant 1 : 100 and A549 supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LAMC2 concentration was determined to be 86,513 pg/mL in A431 supernatant, 3,106 pg/mL in A549 supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human LAMC2 ELISA Kit (AB282301)

Example of human LAMC2 standard curve in Sample Diluent NS.

The LAMC2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human LAMC2 ELISA Kit (AB282301)

Interpolated concentrations of native LAMC2 in human serum, plasma samples.

The concentrations of LAMC2 were measured in duplicates, interpolated from the LAMC2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 25%, plasma (EDTA) neat, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LAMC2 concentration was determined to be 368.1 pg/mL in serum, 366.7 pg/mL in plasma (citrate) and 274.7 pg/mL in plasma (EDTA), and 463.9 pg/mL plasma (heparin).

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.16 pg/mL

Range

25 - 1600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human LAMC2 ELISA kit (ab282301) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human LAMC2 protein in human serum, plasma, cell culture supernatant, and cell extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human LAMC2 with 2.16 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

LAMC2 is the gamma subunit of Laminin 5. Laminin's are complex glycoproteins consisting of an alpha, beta and gamma chain. LAMC2 secreted in lung cancer and associated with the progression of the disease. ab282301 recognizes a fragment of the gamma-2 subunit.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.56" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "96 - 100 %", "average": "= 97" }, { "sample": "Serum", "range": "86 - 109 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "84 - 115 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "86 - 108 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "87 - 116 %", "average": "= 97" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab282301-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB282301-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB282301-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB282301-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB282301-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB282301-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB282301-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human LAMC2 Lyophilized Recombinant Protein", "number":"AB282301-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LAMC2 Capture Antibody", "number":"AB282301-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB282301-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB282301-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LAMC2 Detector Antibody", "number":"AB282301-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LAMC2 protein also known as laminin subunit gamma-2 is an essential component of the extracellular matrix. It is a subunit of the laminin-332 complex formerly referred to as laminin-5 and has an approximate mass of 160 kDa. LAMC2 is widely expressed in various tissues most prominently in epithelial tissues. It localizes at the basement membrane zone where epithelial and mesenchymal tissues meet.

Biological function summary

LAMC2 plays a critical role in cell adhesion migration and signal transduction. Laminin-332 which includes LAMC2 binds to integrin receptors on cell surfaces facilitating cell-matrix interactions and communication. This complex is important for basement membrane stability and tissue organization and it is actively involved in wound healing by promoting keratinocyte migration and melanin transport.

Pathways

LAMC2 integrates into several important extracellular matrix pathways including the integrin signaling pathway. These pathways involve interactions with proteins like integrin alpha-6/beta-4 which are linked to cellular adhesion and mobility. LAMC2 also contributes to epithelial cell functions and is involved in the PI3K/AKT signaling pathway highlighting its importance in cell proliferation and survival.

LAMC2 has significant associations with conditions like dystrophic epidermolysis bullosa and invasive carcinomas. In dystrophic epidermolysis bullosa mutations in LAMC2 disrupt the integrity of the basement membrane leading to fragile skin and blistering. In cancer abnormal expression or alteration of LAMC2 can promote tumor invasion and metastasis often in conjunction with overexpression of matrix metalloproteinases (MMPs) that degrade the extracellular matrix therefore facilitating cancer cell infiltration.

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Product protocols

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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Ladsin exerts cell-scattering activity toward a wide variety of cells, including epithelial, endothelial, and fibroblastic cells.

See full target information LAMC2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 8:250 PubMed39496753

2024

ERO1A levels are a prognostic indicator in EGFR mutated non small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

M A Voronkova,B Johnson,N Gandhi,J M Koomen,M Patrick,S Shanthi Bhupathi,V M Wu,A Elliott,A Vanderwalde,B Halmos,L A Hazlehurst

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human LAMC2 ELISA Kit

Sandwich ELISA - Human LAMC2 ELISA Kit (AB282301)

Sandwich ELISA - Human LAMC2 ELISA Kit (AB282301)

Sandwich ELISA - Human LAMC2 ELISA Kit (AB282301)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise