JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB277457

Human Laminin alpha 4/LAMA4 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Laminin alpha 4/LAMA4 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Laminin alpha 4/LAMA4 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Laminin subunit alpha-4, Laminin-14 subunit alpha, Laminin-8 subunit alpha, Laminin-9 subunit alpha, LAMA4

1 Images
ELISA - Human Laminin alpha 4/LAMA4 ELISA Kit (AB277457)
  • ELISA

Supplier Data

ELISA - Human Laminin alpha 4/LAMA4 ELISA Kit (AB277457)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.63 ng/mL

Range

1.638 - 400 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Laminin alpha 4/LAMA4 ELISA Kit (ab277457) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Laminin alpha 4/LAMA4 in serum (human Laminin alpha 4 concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for Human Laminin alpha 4/LAMA4 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Laminin alpha 4/LAMA4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Laminin alpha 4/LAMA4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Laminin alpha 4/LAMA4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "89 - 142 %", "average": "= 122.1" }, { "sample": "Plasma", "range": "97 - 149 %", "average": "= 119.2" }, { "sample": "Cell culture media", "range": "97 - 140 %", "average": "= 117.8" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277457-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277457-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277457-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Laminin alpha 4 LAMA4 coated Microplate", "number":"AB277457-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277457-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277457-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Laminin alpha 4/LAMA4 standard protein (Lyophilized)", "number":"AB277457-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB277457-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Laminin alpha 4/LAMA4 Antibody", "number":"AB277457-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB277457-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin alpha 4 (LAMA4) also known as laminin-8 is a subunit of the laminin protein family which are essential components of the extracellular matrix. This protein has a molecular weight of approximately 210 kDa. LAMA4 mainly expresses in the vasculature including blood vessels and organs with high metabolic rates like the heart and skeletal muscles. It plays a significant role in maintaining the structural integrity of tissues by forming a network that supports cell adhesion and migration.
Biological function summary

Laminin alpha 4 interacts with other laminin subunits such as beta and gamma chains to form heterotrimeric laminin complexes. These laminins are important for cell differentiation proliferation and tissue repair. LAMA4 influences the basement membrane dynamics contributing to the biomechanical properties of cells. Through its binding to integrins and other cell surface receptors LAMA4 activates intracellular signaling cascades necessary for organogenesis and tissue regeneration.

Pathways

Laminin alpha 4 participates actively in the PI3K/AKT pathway important for cell survival and growth. It also plays a role in the MAPK signaling pathway which is important for cell differentiation and response to stress signals. Through these pathways LAMA4 interacts with various proteins including integrins and dystroglycan modulating essential cellular processes and maintaining cellular homeostasis.

LAMA4's role becomes prominent in cancer progression where altered expression can influence tumor invasion and metastasis. Furthermore it connects to cardiovascular disorders especially in influencing angiogenesis and repair of ischemic tissues. In these contexts LAMA4 relates to proteins like integrin alpha6 and beta1 which facilitate aberrant cellular activities in disease processes. Understanding LAMA4's involvement offers potential pathways for therapeutic intervention in these diseases.
style
left-column

Product protocols

style
left-column

Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
See full target information Laminin subunit alpha-4
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB242198

Anti-Laminin alpha 4/LAMA4 antibody [CL3183]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

  • 2
  • 1
Primary Antibodies

AB78316

Anti-p53 (acetyl K120) antibody [10E5]

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K120) antibody [10E5] (AB78316)

  • 4
  • 4
Assay Kits

AB283541

Dystrophin ELISA Kit

Recombinant|SimpleStep

ELISA - Dystrophin ELISA Kit (AB283541)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com