AB322374

Human Laminin alpha 4/LAMA4 ELISA Kit

Recombinant
SimpleStep
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Human Laminin alpha 4/LAMA4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Laminin alpha 4/LAMA4 with a sensitivity of 6.862 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Immunoglobulin lambda constant 1, Ig lambda chain C region MGC, Ig lambda-1 chain C region, IGLC1

7 Images

Sandwich ELISA - Human Laminin alpha 4/LAMA4 ELISA Kit (AB322374)

sELISA

Supplier Data

Sandwich ELISA - Human Laminin alpha 4/LAMA4 ELISA Kit (AB322374)

Interpolated concentration of native Laminin alpha 4/LAMA4 was measured in duplicate at different sample concentrations. Undiluted samples are 50% HUVEC cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Laminin alpha 4/LAMA4 ELISA Kit (AB322374)

Interpolated concentration of native Laminin alpha 4/LAMA4 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 25% HUVEC cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Laminin alpha 4/LAMA4 ELISA Kit (AB322374)

Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Laminin alpha 4/LAMA4 concentration was determined to be 615.3 pg/mL with a range of 421.7 – 924.0 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Laminin alpha 4/LAMA4 ELISA Kit (AB322374)

Example of human Laminin alpha 4/LAMA4 standard curve in Sample Diluent 50BS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Laminin alpha 4/LAMA4 ELISA Kit (AB322374)

Example of human Laminin alpha 4/LAMA4 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Laminin alpha 4/LAMA4 ELISA Kit (AB322374)

Example of human Laminin alpha 4/LAMA4 standard curve in Sample Diluent 50BS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Laminin alpha 4/LAMA4 ELISA Kit (AB322374)

Example of human Laminin alpha 4/LAMA4 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6.862 pg/mL

Range

39.063 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Laminin alpha 4/LAMA4 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Laminin alpha 4/LAMA4 protein in Serum, Cit Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human Laminin alpha 4/LAMA4 with 6.862 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.4" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.2" } ]

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Recovery

[ { "sample": "Citrate plasma", "range": "92 - 97 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "91 - 104 %", "average": "= 97" }, { "sample": "Heparin Plasma", "range": "99 - 106 %", "average": "= 101" }, { "sample": "Cell culture supernatant", "range": "110 - 114 %", "average": "= 112" }, { "sample": "Saliva", "range": "100 - 127 %", "average": "= 113" }, { "sample": "Milk", "range": "93 - 97 %", "average": "= 95" }, { "sample": "Cerebral Spinal Fluid", "range": "102 - 105 %", "average": "= 104" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).

See full target information Immunoglobulin lambda constant 1

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websiteProtocolBooklet

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Product promise

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