Human Laminin alpha 4/LAMA4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Laminin alpha 4/LAMA4 with a sensitivity of 6.862 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, Heparin Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 39.063 - 2500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 6.862 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Alternative names
Immunoglobulin lambda constant 1, Ig lambda chain C region MGC, Ig lambda-1 chain C region, IGLC1
What's included?
Human Laminin alpha 4/LAMA4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Laminin alpha 4/LAMA4 with a sensitivity of 6.862 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, Heparin Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 39.063 - 2500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 6.862 pg/mL
- General recovery
- 97%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 8 | mean - | SD - | C.V. = 3.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 3 | mean - | SD - | C.V. = 3.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Citrate plasma | Average % = 94 | Range 92 - 97 % |
Sample type EDTA Plasma | Average % = 97 | Range 91 - 104 % |
Sample type Heparin Plasma | Average % = 101 | Range 99 - 106 % |
Sample type Cell culture supernatant | Average % = 112 | Range 110 - 114 % |
Sample type Saliva | Average % = 113 | Range 100 - 127 % |
Sample type Milk | Average % = 95 | Range 93 - 97 % |
Sample type Cerebral Spinal Fluid | Average % = 104 | Range 102 - 105 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Laminin alpha 4/LAMA4 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Laminin alpha 4/LAMA4 protein in Serum, Cit Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human Laminin alpha 4/LAMA4 with 6.862 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
Sandwich ELISA - Human Laminin alpha 4/LAMA4 ELISA Kit (ab322374)
Interpolated concentration of native Laminin alpha 4/LAMA4 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 25% HUVEC cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Laminin alpha 4/LAMA4 ELISA Kit (ab322374)
Example of human Laminin alpha 4/LAMA4 standard curve in Sample Diluent 50BS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Laminin alpha 4/LAMA4 ELISA Kit (ab322374)
Example of human Laminin alpha 4/LAMA4 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Laminin alpha 4/LAMA4 ELISA Kit (ab322374)
Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Laminin alpha 4/LAMA4 concentration was determined to be 615.3 pg/mL with a range of 421.7 – 924.0 pg/mL.
Sandwich ELISA - Human Laminin alpha 4/LAMA4 ELISA Kit (ab322374)
Interpolated concentration of native Laminin alpha 4/LAMA4 was measured in duplicate at different sample concentrations. Undiluted samples are 50% HUVEC cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Laminin alpha 4/LAMA4 ELISA Kit (ab322374)
Example of human Laminin alpha 4/LAMA4 standard curve in Sample Diluent 50BS. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Laminin alpha 4/LAMA4 ELISA Kit (ab322374)
Example of human Laminin alpha 4/LAMA4 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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