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AB288588

Human Laminin Beta 2 ELISA Kit

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(1 Publication)

Human Laminin Beta 2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Laminin Beta 2 with a sensitivity of 2.498 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

LAMS, LAMB2, Laminin subunit beta-2, Laminin B1s chain, Laminin-11 subunit beta, Laminin-14 subunit beta, Laminin-15 subunit beta, Laminin-3 subunit beta, Laminin-4 subunit beta, Laminin-7 subunit beta, Laminin-9 subunit beta, S-laminin subunit beta, S-LAM beta

4 Images
ELISA - Human Laminin Beta 2 ELISA Kit (AB288588)
  • ELISA

Supplier Data

ELISA - Human Laminin Beta 2 ELISA Kit (AB288588)
Sandwich ELISA - Human Laminin Beta 2 ELISA Kit (AB288588)
  • sELISA

Supplier Data

Sandwich ELISA - Human Laminin Beta 2 ELISA Kit (AB288588)

Interpolated concentration of native Laminin Beta 2 was measured in duplicate at different sample concentrations. Undiluted samples are 25% HepG2 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Laminin Beta 2 ELISA Kit (AB288588)
  • sELISA

Supplier Data

Sandwich ELISA - Human Laminin Beta 2 ELISA Kit (AB288588)

Interpolated concentration of native Laminin Beta 2 was measured in duplicate at different sample concentrations. Undiluted samples are 5% serum, plasma (heparin), plasma (EDTA), and plasma (citrate). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Laminin Beta 2 ELISA Kit (AB288588)
  • sELISA

Supplier Data

Sandwich ELISA - Human Laminin Beta 2 ELISA Kit (AB288588)

Example of human Laminin Beta 2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.498 pg/mL

Range

6.25 - 400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Laminin Beta 2 ELISA Kit ab288588 is a rapid single-wash 90-min Sandwich ELISA to measure Human Laminin Beta 2 in cell culture supernatant, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 2.498 pg/mL.

How the assay works

Human Laminin Beta 2 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Laminin Beta 2 ELISA Kit ab288588 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Laminin beta 2 antibody [EPR23569-8] - BSA and Azide free (Capture) ab281074
- Anti-Laminin beta 2 antibody [EPR23569-82] - BSA and Azide free (Detector) ab281074
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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 113 %", "average": "= 103" }, { "sample": "Serum", "range": "105 - 107 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "105 - 119 %", "average": "= 113" }, { "sample": "Heparin Plasma", "range": "102 - 109 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "101 - 115 %", "average": "= 110" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin beta 2 also known as laminin B2 or laminin beta chain is an important component of the extracellular matrix. This laminin molecule has a molecular weight of approximately 210 kDa. It forms an essential part of the structural integrity in tissues by serving as a basement membrane protein. Expression of laminin beta 2 occurs extensively in the kidney peripheral nerves and neuromuscular junctions. It plays a role in binding other laminin subunits to form functional heterotrimeric laminin molecules.
Biological function summary

Laminin beta 2 is involved in the formation and maintenance of the basement membrane structure contributing to tissue cohesion and cell adhesion. It is part of the laminin-521 complex which involves two other chains that together foster cellular adhesion migration and differentiation. This laminin molecule supports developmental processes by providing signaling cues that influence the behavior of various cell types making it indispensable in organ development and repair.

Pathways

Laminin beta 2 interacts within the integrin signaling pathway where it mediates cell adhesion and communication. It also plays an important role in the basement membrane assembly pathway interacting closely with integrin α3β1. Through these pathways laminin beta 2 maintains cellular organization and stability reflecting its integrative role together with related proteins like nidogen and collagen IV.

Laminin beta 2 mutations link to Pierson syndrome a condition characterized by kidney and eye abnormalities. Additionally abnormalities in laminin beta 2 expression have associations with congenital muscular dystrophy where the integrity of neuromuscular junctions is compromised. Genetic studies connect it with the dystroglycan protein highlighting its significance in maintaining tissue architecture and function in the context of these disorders.
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Product protocols

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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
See full target information LAMB2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 20:5531-5547 PubMed39494341

2024

Reconstruction of the Hepatic Microenvironment and Pathological Changes Underlying Type II Diabetes through Single-Cell RNA Sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Yen Dai,Ying-Ming Tsai,Chao-Yuan Chang,Hung-Pei Tsai,Kuan-Li Wu,Yu-Yuan Wu,Ling-Yu Wu,Shu-Fang Jian,Pei-Hsun Tsai,Chai-Tung Ong,Chien-Hui Sun,Ya-Ling Hsu
View all publications
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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