Human Laminin Beta 2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Laminin Beta 2 with a sensitivity of 2.498 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 6.25 - 400 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 2.498 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Alternative names
LAMS, LAMB2, Laminin subunit beta-2, Laminin B1s chain, Laminin-11 subunit beta, Laminin-14 subunit beta, Laminin-15 subunit beta, Laminin-3 subunit beta, Laminin-4 subunit beta, Laminin-7 subunit beta, Laminin-9 subunit beta, S-laminin subunit beta, S-LAM beta
What's included?
Recommended products
Human Laminin Beta 2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Laminin Beta 2 with a sensitivity of 2.498 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 6.25 - 400 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 2.498 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 6.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 103 | Range 84 - 113 % |
Sample type Serum | Average % = 106 | Range 105 - 107 % |
Sample type EDTA Plasma | Average % = 113 | Range 105 - 119 % |
Sample type Heparin Plasma | Average % = 105 | Range 102 - 109 % |
Sample type Citrate plasma | Average % = 110 | Range 101 - 115 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Laminin Beta 2 ELISA Kit (ab288588) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Laminin Beta 2 protein in EDTA plasma, Hep plasma, Cell culture supernatant, serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Laminin Beta 2 with 2.498 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Laminin beta 2 also known as laminin B2 or laminin beta chain is an important component of the extracellular matrix. This laminin molecule has a molecular weight of approximately 210 kDa. It forms an essential part of the structural integrity in tissues by serving as a basement membrane protein. Expression of laminin beta 2 occurs extensively in the kidney peripheral nerves and neuromuscular junctions. It plays a role in binding other laminin subunits to form functional heterotrimeric laminin molecules.
- Biological function summary
Laminin beta 2 is involved in the formation and maintenance of the basement membrane structure contributing to tissue cohesion and cell adhesion. It is part of the laminin-521 complex which involves two other chains that together foster cellular adhesion migration and differentiation. This laminin molecule supports developmental processes by providing signaling cues that influence the behavior of various cell types making it indispensable in organ development and repair.
- Pathways
Laminin beta 2 interacts within the integrin signaling pathway where it mediates cell adhesion and communication. It also plays an important role in the basement membrane assembly pathway interacting closely with integrin α3β1. Through these pathways laminin beta 2 maintains cellular organization and stability reflecting its integrative role together with related proteins like nidogen and collagen IV.
- Associated diseases and disorders
Laminin beta 2 mutations link to Pierson syndrome a condition characterized by kidney and eye abnormalities. Additionally abnormalities in laminin beta 2 expression have associations with congenital muscular dystrophy where the integrity of neuromuscular junctions is compromised. Genetic studies connect it with the dystroglycan protein highlighting its significance in maintaining tissue architecture and function in the context of these disorders.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Sandwich ELISA - Human Laminin Beta 2 ELISA Kit (ab288588)
Example of human Laminin Beta 2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Laminin Beta 2 ELISA Kit (ab288588)
Interpolated concentration of native Laminin Beta 2 was measured in duplicate at different sample concentrations. Undiluted samples are 25% HepG2 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Laminin Beta 2 ELISA Kit (ab288588)
Interpolated concentration of native Laminin Beta 2 was measured in duplicate at different sample concentrations. Undiluted samples are 5% serum, plasma (heparin), plasma (EDTA), and plasma (citrate). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com