AB327295
Human Laminin beta 2 ELISA Kit, Chemiluminescent
- SimpleStep Ignite™ ELISA
- Recombinant
- What is this?
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Human Laminin beta 2 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Laminin beta 2 with higher sensitivity and broader measuring range.
- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 1.65 - 1200 pg/ml with a sensitivity of 0.43 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 1.65 - 1200 pg/ml with a sensitivity of 0.43 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
4 Images
- sELISA
Supplier Data
Sandwich ELISA - Human Laminin beta 2 ELISA Kit, Chemiluminescent (AB327295)
Interpolated concentration of native Laminin Beta 2 was measured in duplicate at different sample concentrations. Undiluted samples are 25% HepG2 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Human Laminin beta 2 ELISA Kit, Chemiluminescent (AB327295)
Interpolated concentration of native Laminin Beta 2 was measured in duplicate at different sample concentrations. Undiluted samples are 5% serum plasma (heparin) plasma (EDTA) and plasma (citrate). The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Lab
Sandwich ELISA - Human Laminin beta 2 ELISA Kit, Chemiluminescent (AB327295)
Standard curve comparison between the Chemiluminescent Human Laminin Beta 2 SimpleStep ELISA and colorimetric Human Laminin Beta 2 SimpleStep ELISA (ab288588) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Lab
Sandwich ELISA - Human Laminin beta 2 ELISA Kit, Chemiluminescent (AB327295)
Dynamic range comparison between the Chemiluminescent Human Laminin Beta 2 SimpleStep ELISA, colorimetric Human Laminin Beta 2 SimpleStep ELISA (ab288588), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.
Reactivity data
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Product details
Human Laminin beta 2 ELISA Kit, Chemiluminescent (ab327295) is a rapid single-wash sandwich 55-min ELISA to measure Human Laminin beta 2 in Cell culture supernatant, Serum, EDTA Plasma, Heparin Plasma, Citrate plasma with a sensitivity of 0.43 pg/mL.
How the assay works
Human Laminin beta 2 ELISA Kit, Chemiluminescent (ab327295) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Laminin beta 2 (ab327295) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Laminin subunit beta-2 antibody [EPR23569-8] - BSA and Aside free (Capture) ab281224
- Anti-Laminin subunit beta-2 antibody [EPR23569-82] - BSA and Aside free (Detector) ab281074
How the assay works
Human Laminin beta 2 ELISA Kit, Chemiluminescent (ab327295) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Laminin beta 2 (ab327295) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Laminin subunit beta-2 antibody [EPR23569-8] - BSA and Aside free (Capture) ab281224
- Anti-Laminin subunit beta-2 antibody [EPR23569-82] - BSA and Aside free (Detector) ab281074
Precision
[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9" } ]
Recovery
[ { "sample": "Cell culture supernatant", "range": "84 - 113 %", "average": "= 103" }, { "sample": "Serum", "range": "105 - 107 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "105 - 119 %", "average": "= 113" }, { "sample": "Heparin Plasma", "range": "102 - 109 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "101 - 115 %", "average": "= 110" } ]
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Alternative Names
LAMS, LAMB2, Laminin subunit beta-2, Laminin B1s chain, Laminin-11 subunit beta, Laminin-14 subunit beta, Laminin-15 subunit beta, Laminin-3 subunit beta, Laminin-4 subunit beta, Laminin-7 subunit beta, Laminin-9 subunit beta, S-laminin subunit beta, S-LAM beta
Product promise
We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com