Human Laminin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Laminin in Human in Cell culture supernatant, Serum, Cell Lysate samples.
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- International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 115:134-1412021Serum levels of laminin and von Willebrand factor in COVID-19 survivors 6 months after discharge.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongwei Li,Qian Wu,Zhonghua Qin,Xinwei Hou,Limin Zhang,Jin Guo,Yajie Li,Fangfei Yang,Yan Zhang,Qi Wu,Li Li,Huaiyong ChenPubMed 34843955
- International journal of molecular sciences 21:2020Influence of Implant Material and Surface on Mode and Strength of Cell/Matrix Attachment of Human Adipose Derived Stromal Cell.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSusanne Jung et. al.PubMed 32526920
- Biomaterials 182:312-3222018Lineage-specific exosomes could override extracellular matrix mediated human mesenchymal stem cell differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarthikeyan Narayanan et. al.PubMed 30153612
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Serum, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 156 - 10000 pg/mL
- Sensitivity
- < 10 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Alternative names
LAMS, LAMB2, Laminin subunit beta-2, Laminin B1s chain, Laminin-11 subunit beta, Laminin-14 subunit beta, Laminin-15 subunit beta, Laminin-3 subunit beta, Laminin-4 subunit beta, Laminin-7 subunit beta, Laminin-9 subunit beta, S-laminin subunit beta, S-LAM beta
What's included?
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Human Laminin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Laminin in Human in Cell culture supernatant, Serum, Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Serum, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 156 - 10000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 10 pg/mL
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human Laminin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Laminin in cell culture supernatants, serum and plasma (heparin, EDTA).
A Laminin specific rabbit polyclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from rabbit specific for Laminin is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates ware washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Laminin amount of sample captured in plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Laminin beta 2 also known as laminin B2 or laminin beta chain is an important component of the extracellular matrix. This laminin molecule has a molecular weight of approximately 210 kDa. It forms an essential part of the structural integrity in tissues by serving as a basement membrane protein. Expression of laminin beta 2 occurs extensively in the kidney peripheral nerves and neuromuscular junctions. It plays a role in binding other laminin subunits to form functional heterotrimeric laminin molecules.
- Biological function summary
Laminin beta 2 is involved in the formation and maintenance of the basement membrane structure contributing to tissue cohesion and cell adhesion. It is part of the laminin-521 complex which involves two other chains that together foster cellular adhesion migration and differentiation. This laminin molecule supports developmental processes by providing signaling cues that influence the behavior of various cell types making it indispensable in organ development and repair.
- Pathways
Laminin beta 2 interacts within the integrin signaling pathway where it mediates cell adhesion and communication. It also plays an important role in the basement membrane assembly pathway interacting closely with integrin α3β1. Through these pathways laminin beta 2 maintains cellular organization and stability reflecting its integrative role together with related proteins like nidogen and collagen IV.
- Associated diseases and disorders
Laminin beta 2 mutations link to Pierson syndrome a condition characterized by kidney and eye abnormalities. Additionally abnormalities in laminin beta 2 expression have associations with congenital muscular dystrophy where the integrity of neuromuscular junctions is compromised. Genetic studies connect it with the dystroglycan protein highlighting its significance in maintaining tissue architecture and function in the context of these disorders.
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2 product images
Sandwich ELISA - Human Laminin ELISA Kit (ab119599)
Hu laminin measured in various biological fluids showing quantity (pg) per mL of tested sample
Sandwich ELISA - Human Laminin ELISA Kit (ab119599)
Standard curve: mean of duplicates (+/-SD) with background readings subtracted
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Product protocols
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