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AB119599

Human Laminin ELISA Kit

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(3 Publications)

Human Laminin ELISA Kit is a sandwich ELISA designed to quantify Human Laminin with a sensitivity of 10 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 156 - 10000 pg/mL

View Alternative Names

LAMS, LAMB2, Laminin subunit beta-2, Laminin B1s chain, Laminin-11 subunit beta, Laminin-14 subunit beta, Laminin-15 subunit beta, Laminin-3 subunit beta, Laminin-4 subunit beta, Laminin-7 subunit beta, Laminin-9 subunit beta, S-laminin subunit beta, S-LAM beta

2 Images
Sandwich ELISA - Human Laminin ELISA Kit (AB119599)
  • sELISA

Lab

Sandwich ELISA - Human Laminin ELISA Kit (AB119599)

Hu laminin measured in various biological fluids showing quantity (pg) per mL of tested sample

Sandwich ELISA - Human Laminin ELISA Kit (AB119599)
  • sELISA

Lab

Sandwich ELISA - Human Laminin ELISA Kit (AB119599)

Standard curve : mean of duplicates (+/-SD) with background readings subtracted

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum, Cell Lysate, Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

156 - 10000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Laminin ELISA Kit ab119599 is a sandwich ELISA to measure Human Laminin in serum, cell culture supernatant, cell lysate with a sensitivity of 10 pg/ml.

How the assay works

A Laminin specific rabbit polyclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from rabbit specific for Laminin is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates ware washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Laminin amount of sample captured in plate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Laminin ELISA Kit ab119599 protocol summary

1. Add standard or sample to each well used. Incubate 37C
2. Add prepared biotin antibody to each well. Incubate at 37C
3. Add prepared Avidin-BiotinPeroxidase Complex (ABC). Incubate at 37C
4. Add TMB to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450 nm
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin beta 2 also known as laminin B2 or laminin beta chain is an important component of the extracellular matrix. This laminin molecule has a molecular weight of approximately 210 kDa. It forms an essential part of the structural integrity in tissues by serving as a basement membrane protein. Expression of laminin beta 2 occurs extensively in the kidney peripheral nerves and neuromuscular junctions. It plays a role in binding other laminin subunits to form functional heterotrimeric laminin molecules.
Biological function summary

Laminin beta 2 is involved in the formation and maintenance of the basement membrane structure contributing to tissue cohesion and cell adhesion. It is part of the laminin-521 complex which involves two other chains that together foster cellular adhesion migration and differentiation. This laminin molecule supports developmental processes by providing signaling cues that influence the behavior of various cell types making it indispensable in organ development and repair.

Pathways

Laminin beta 2 interacts within the integrin signaling pathway where it mediates cell adhesion and communication. It also plays an important role in the basement membrane assembly pathway interacting closely with integrin α3β1. Through these pathways laminin beta 2 maintains cellular organization and stability reflecting its integrative role together with related proteins like nidogen and collagen IV.

Laminin beta 2 mutations link to Pierson syndrome a condition characterized by kidney and eye abnormalities. Additionally abnormalities in laminin beta 2 expression have associations with congenital muscular dystrophy where the integrity of neuromuscular junctions is compromised. Genetic studies connect it with the dystroglycan protein highlighting its significance in maintaining tissue architecture and function in the context of these disorders.
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Product protocols

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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
See full target information LAMB2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 115:134-141 PubMed34843955

2021

Serum levels of laminin and von Willebrand factor in COVID-19 survivors 6 months after discharge.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwei Li,Qian Wu,Zhonghua Qin,Xinwei Hou,Limin Zhang,Jin Guo,Yajie Li,Fangfei Yang,Yan Zhang,Qi Wu,Li Li,Huaiyong Chen

International journal of molecular sciences 21: PubMed32526920

2020

Influence of Implant Material and Surface on Mode and Strength of Cell/Matrix Attachment of Human Adipose Derived Stromal Cell.

Applications

Unspecified application

Species

Unspecified reactive species

Susanne Jung,Lauren Bohner,Marcel Hanisch,Johannes Kleinheinz,Sonja Sielker

Biomaterials 182:312-322 PubMed30153612

2018

Lineage-specific exosomes could override extracellular matrix mediated human mesenchymal stem cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Karthikeyan Narayanan,Sundramurthy Kumar,Parasuraman Padmanabhan,Balazs Gulyas,Andrew C A Wan,Vazhaikkurichi M Rajendran
View all publications
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websiteProtocolBooklet
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