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Human LAMP1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human LAMP1 in Human in Plasma, Cell culture supernatant, Serum samples.

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ELISA - Human LAMP1 ELISA Kit (AB277464), expandable thumbnail

Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell culture supernatant, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
1.229 - 300 ng/mL
Sensitivity
= 1 ng/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis (PubMed:37390818). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (PubMed:37390818). Also plays an important role in NK-cells cytotoxicity (PubMed:2022921, PubMed:23632890). Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (PubMed:23632890). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed:23847195). Presents carbohydrate ligands to selectins (PubMed:7685349). (Microbial infection) Acts as a receptor for Lassa virus glycoprotein (PubMed:24970085, PubMed:25972533, PubMed:27605678, PubMed:28448640). Promotes also fusion of the virus with host membrane in less acidic endosomes (PubMed:29295909). (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
350X HRP-Streptavidin Concentrate
1 x 200 µL
5X Assay Diluent B
1 x 15 mL
Anti-Human LAMP1 coated Microplate
1 x 1 Unit
Assay Diluent C
1 x 30 mL
Biotinylated Anti-Human LAMP1 Antibody
2 x 1 Vial
Human LAMP1 standard protein (Lyophilized)
2 x 1 Vial
Stop Solution
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human LAMP1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human LAMP1 in Human in Plasma, Cell culture supernatant, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell culture supernatant, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
1.229 - 300 ng/mL
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 1 ng/mL

Precision

Intra assay

Sample
Overall
n
0
C.V.
< 10

Inter assay

Sample
Overall
n
0
C.V.
< 12

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 89.24
Range
69 - 114 %
Sample type
Plasma
Average %
= 92.01
Range
76 - 102 %
Sample type
Cell culture media
Average %
= 116.9
Range
100 - 143 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Human LAMP1 ELISA Kit (ab277464) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LAMP1 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human LAMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human LAMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human LAMP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human LAMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.

Biological function summary

LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.

Pathways

The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.

Associated diseases and disorders

LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.

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1 product image

  • ELISA - Human LAMP1 ELISA Kit (ab277464), expandable thumbnail

    ELISA - Human LAMP1 ELISA Kit (ab277464)

    These standard curves are for demonstration only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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