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AB313981

Human LAYN ELISA Kit

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Human LAYN ELISA Kit is a Sandwich ELISA for the measurement of Human LAYN in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

UNQ208/PRO234, LAYN, Layilin

1 Images
Sandwich ELISA - Human LAYN ELISA Kit (AB313981)
  • sELISA

Supplier Data

Sandwich ELISA - Human LAYN ELISA Kit (AB313981)

Example of standard curve using ab313981. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.012 ng/mL

Range

0.012 - 10 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB192610

Anti-LAYN antibody [EPR11875(2)]

RabMAb|Recombinant

Western blot - Anti-LAYN antibody [EPR11875(2)] (AB192610)

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  • 1
Proteins & Peptides

AB276229

Recombinant Human LAYN protein (Fc Chimera)

SDS-PAGE - Recombinant Human LAYN protein (Fc Chimera) (AB276229)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human LAYN ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human LAYN in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human LAYN coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ANGPTL3, beta IG-H3, CA9, Cathepsin B, CD23, CD26 (DPPIV), CHI3L1, Coagulation Factor III (Tissue Factor/Thromboplastin), CTLA-4 (CD152), Dkk-4, EDA-A2, Erythropoietin R, FGF-6, FGF-9, FOLH1 (PSMA), Gas 1, IGFBP-5, IL-38 (IL-1 F10), IL-36 Ra (IL-1 F5), IL36 alpha (IL1 F6), IL-37 (IL-1 F7), IL-36 beta (IL-1 F8), IL-36 gamma (IL1 F9), IL-1 R5 (IL-18 R alpha), IL-10 R alpha, IL-17C, IL-18, IL-20, IL-34, IL-5 R alpha, Leptin R, Marapsin, Mer, MMP-7, P-Cadherin, Prostasin, SIGIRR, TGF beta R3, TWEAK (TNFSF12)

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Recovery

[ { "sample": "Serum", "range": "112 - 139 %", "average": "= 123.4" }, { "sample": "Plasma", "range": "120 - 139 %", "average": "= 130.5" }, { "sample": "Cell culture media", "range": "118 - 140 %", "average": "= 133.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313981-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313981-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313981-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human LAYN Antibody-coated ELISA Plate", "number":"AB313981-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human LAYN Detection Antibody", "number":"AB313981-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313981-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"250X HRP-Streptavidin", "number":"AB313981-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313981-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human LAYN Protein Standard", "number":"AB313981-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LAYN protein also known as layilin plays a role in cell adhesion and signal transduction. It is an approximately 55 kDa protein expressed mainly in epithelial and immune cells including T-cells and natural killer cells. This protein sits on the cell surface and has a connection to the cytoskeleton through its cytoplasmic tail. Alternate expression may occur in both normal and cancerous tissues indicating a role in various physiological and pathological processes.
Biological function summary

LAYN facilitates interactions between cells and the extracellular matrix. It acts as a receptor for hyaluronic acid contributing to cell migration and tissue remodeling. While it is not part of any specific complex layilin may interact with other extracellular matrix proteins to promote cellular activities. This interaction influences immune responses by modulating immune cell activation and homing.

Pathways

LAYN is involved in the hyaluronan-mediated motility pathway where it binds hyaluronic acid to regulate cell movement. Additionally the protein takes part in the immune response pathway by influencing immune cell behavior. LAYN interacts with proteins like moesin which helps connect the cell membrane to the actin cytoskeleton driving cellular changes during these processes.

LAYN has a connection to cancer progression and autoimmune diseases. It plays a role in the tumor microenvironment affecting tumor cell motility and can influence metastasis. In autoimmune disorders LAYN's role in modulating immune cell activity can trigger aberrant immune responses. The protein interacts with various components of the immune system linking its expression to both health and disease states.
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Product protocols

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Target data

Receptor for hyaluronate.
See full target information LAYN
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websiteProtocolBooklet
en
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Product promise

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