AB279407

Human LBP ELISA Kit (LPS Binding Protein)

Recombinant
SimpleStep
What is this?

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(5 Publications)

Human LBP ELISA Kit (LPS Binding Protein) is a single-wash 90-min Simplestep used to quantify Human LBP (LPS Binding Protein) with a sensitivity of 6.79 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Lipopolysaccharide-binding protein, LBP

4 Images

ELISA - Human LBP ELISA Kit (LPS Binding Protein) (AB279407)

ELISA

Supplier Data

ELISA - Human LBP ELISA Kit (LPS Binding Protein) (AB279407)

sELISA

Supplier Data

Sandwich ELISA - Human LBP ELISA Kit (LPS Binding Protein) (AB279407)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LBP concentration was determined to be 3.11 μg/mL with a range of 0.94 – 4.60 μg/ml.

sELISA

Supplier Data

Sandwich ELISA - Human LBP ELISA Kit (LPS Binding Protein) (AB279407)

Interpolated concentrations of native LBP in human serum and plasma samples.

The concentrations of LBP were measured in duplicates, interpolated from the LBP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 4,000, plasma (citrate) 1 : 2,000, plasma (heparin) 1 : 2,000, and plasma (EDTA) 1 : 2,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LBP concentration was determined to be 2.00 μg/mL in serum, 1.39 μg/mL in plasma (citrate), 1.01 μg/mL in plasma (heparin), and 1.50 μg/ml in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Human LBP ELISA Kit (LPS Binding Protein) (AB279407)

Example of human LBP standard curve in Sample Diluent NS.

The LBP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. The LBP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 6.79 pg/mL

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human LBP ELISA Kit (LPS Binding Protein) ab279407 is a rapid single-wash 90-min Sandwich ELISA to measure Human LBP (LPS Binding Protein)in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 6.79 pg/mL.

How the assay works

Human LBP (LPS Binding Protein)SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human LBP ELISA Kit (LPS Binding Protein) ab279407 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

LBP (LPS Binding Protein) is an acute phase protein involved in the cellular response to gram-negative bacterial infections. LBP binds to LPS protein expressed on gram-negative bacteria and elicits a response by engaging with pattern recognition receptors, CD14 and TLR4. LBP is essential for the initial response to gram-negative bacterial infection but not for the downstream stages.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" } ]

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Recovery

[ { "sample": "Serum", "range": "97 - 112 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "93 - 97 %", "average": "= 95" }, { "sample": "Cell culture media", "range": "86 - 93 %", "average": "= 90" }, { "sample": "Heparin Plasma", "range": "93 - 108 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "88 - 100 %", "average": "= 94" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab279407-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB279407-CMP03" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB279407-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB279407-CMP06" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB279407-CMP07" }, { "size":"2 x 10 Vial", "name":"Human LBP (LPS Binding Protein) Lyophilized Recombinant Protein", "number":"AB279407-CMP08" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB279407-CMP01" }, { "size":"1 x 6000 µL", "name":"10X Human LBP (LPS Binding Protein) Capture Antibody", "number":"AB279407-CMP04" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB279407-CMP09" }, { "size":"1 x 6000 µL", "name":"10X Human LBP (LPS Binding Protein) Detector Antibody", "number":"AB279407-CMP05" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB279407-CMP02" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279407-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB279407-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB279407-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB279407-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB279407-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB279407-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB279407-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human LBP (LPS Binding Protein) Lyophilized Recombinant Protein", "number":"AB279407-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LBP (LPS Binding Protein) Capture Antibody", "number":"AB279407-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LBP (LPS Binding Protein) Detector Antibody", "number":"AB279407-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB279407-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lipopolysaccharide-binding protein (LBP) also known as LPS-binding protein is an acute-phase protein that plays a critical role in the immune response to Gram-negative bacterial infections. This protein weighing approximately 60kDa is primarily expressed in the liver and found in the serum. LBP binds to lipopolysaccharides (LPS) which are components of the outer membrane of Gram-negative bacteria facilitating the transfer of LPS to CD14 receptors on immune cells. This transfer enhances the recognition of bacterial endotoxins triggering an immune response.

Biological function summary

LBP serves a significant role in enhancing the sensitivity of the immune system to bacterial endotoxins. It does not form a permanent part of a larger complex but works closely with other molecules like CD14 and MD-2 an accessory protein of the TLR4 receptor to mediate the innate immune response. By efficiently binding LPS LBP aids in transporting these molecules to cell surface receptors accelerating the detection and subsequent response to infections.

Pathways

LBP is heavily involved in the TLR4 signaling pathway which is important for the detection and response to Gram-negative bacterial infections. Through this pathway LBP and associated proteins such as CD14 and MD-2 help activate TLR4 triggering downstream NF-κB signaling. This activation leads to the production of pro-inflammatory cytokines and an upsurge in antimicrobial activities fortifying the body's defenses against bacterial invasion.

Enhanced LBP levels have been associated with sepsis a severe systemic response to bacterial infections which can often lead to septic shock. Given its pivotal role in recognizing bacterial endotoxins LBP is important in identifying infections making it a potential prospect for therapeutic targeting. Additionally its interaction with CD14 means that alterations in LBP function or expression levels might contribute to immune dysregulation seen in inflammatory diseases such as Crohn's disease where immune responses to gut microbiota play a central role.

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Product protocols

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Target data

Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria (PubMed : 24120359, PubMed : 7517398). Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide (PubMed : 24120359, PubMed : 7517398).

See full target information LBP

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of chronic obstructive pulmonary disease 20:2049-2059 PubMed40585425

2025

The Interface of a Leaky Gut with Reduced Sarcopenia-Related Quality of Life (SarQoL) in Patients with Chronic Obstructive Pulmonary Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Rizwan Qaisar,Asima Karim,Tahir Muhammad,Firdos Ahmad,Daniel Almeida Marinho,Ioannis Arkadianos,Shaea Alkahtani

Frontiers in microbiology 15:1407555 PubMed39184030

2024

Bariatric-induced microbiome changes alter MASLD development in association with changes in the innate immune system.

Applications

Unspecified application

Species

Unspecified reactive species

Simer Shera,William Katzka,Julianne C Yang,Candace Chang,Nerea Arias-Jayo,Venu Lagishetty,Anna Balioukova,Yijun Chen,Erik Dutson,Zhaoping Li,Emeran A Mayer,Joseph R Pisegna,Claudia Sanmiguel,Shrey Pawar,David Zhang,Madelaine Leitman,Laura Hernandez,Jonathan P Jacobs,Tien S Dong

Clinical and translational gastroenterology 15:e00697 PubMed38488171

2024

Bacterial DNA Translocation Is Associated With Overt Hepatic Encephalopathy and Mortality in Patients With Cirrhosis.

Applications

Unspecified application

Species

Unspecified reactive species

Kessarin Thanapirom,Sirinporn Suksawatamnuay,Salisa Wejnaruemarn,Panarat Thaimai,Nipaporn Siripon,Prooksa Ananchuensook,Supachaya Sriphoosanaphan,Jakapat Vanichanan,Sombat Treeprasertsuk,Yong Poovorawan,Piyawat Komolmit

Nutrition research and practice 17:883-898 PubMed37780220

2023

Probiotic supplementation has sex-dependent effects on immune responses in association with the gut microbiota in community-dwelling older adults: a randomized, double-blind, placebo-controlled, multicenter trial.

Applications

Unspecified application

Species

Unspecified reactive species

Chong-Su Kim,Min Ho Jung,Eun Young Choi,Dong-Mi Shin

Gut microbes 15:2167170 PubMed36732495

2023

Microbial changes from bariatric surgery alters glucose-dependent insulinotropic polypeptide and prevents fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tien S Dong,William Katzka,Julianne C Yang,Candace Chang,Nerea Arias-Jayo,Venu Lagishetty,Anna Balioukova,Yijun Chen,Erik Dutson,Zhaoping Li,Emeran A Mayer,Joseph R Pisegna,Claudia Sanmiguel,Jonathan P Jacobs

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human LBP ELISA Kit (LPS Binding Protein)

ELISA - Human LBP ELISA Kit (LPS Binding Protein) (AB279407)

Sandwich ELISA - Human LBP ELISA Kit (LPS Binding Protein) (AB279407)

Sandwich ELISA - Human LBP ELISA Kit (LPS Binding Protein) (AB279407)

Sandwich ELISA - Human LBP ELISA Kit (LPS Binding Protein) (AB279407)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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