AB239432

Human LC3A ELISA Kit

Recombinant
SimpleStep
What is this?

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(2 Publications)

Human LC3A ELISA Kit is a single-wash 90-min Simplestep used to quantify Human LC3A with a sensitivity of 2.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1A/MAP1B LC3 A, MAP1LC3A

4 Images

Sandwich ELISA - Human LC3A ELISA Kit (AB239432)

sELISA

Unknown

Sandwich ELISA - Human LC3A ELISA Kit (AB239432)

Comparison of LC3A in mock-treated and chloroquine-treated HeLa cell extracts. HeLa cells were cultured for 24 hours in the absence (mock) or presence of 50 mM chloroquine. The concentrations of LC3A were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the LC3A standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean LC3A concentration was determined to be 3.16 ng/mg in mock-treated HeLa cell extract, and 5.64 ng/mg in chloroquine-treated HeLa cell extract.

sELISA

Supplier Data

Sandwich ELISA - Human LC3A ELISA Kit (AB239432)

Representative image of Human LC3A ELISA Kit (ab239432)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human LC3A ELISA Kit Capture Antibody

- 10X Human LC3A Detector Antibody

- 10X Human LC3A ELISA Kit Lyophilised Purified Protein (2 vials)

- Human LC3A ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Unknown

Sandwich ELISA - Human LC3A ELISA Kit (AB239432)

Interpolated concentrations of native LC3A in human SHSY-5Y cell extract based on a 30 μg/mL extract load, and HeLa cell extract based on a 500 μg/mL extract load. The concentrations of LC3A were measured in duplicate and interpolated from the LC3A standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LC3A concentration was determined to be 2,048 pg/mL in SHSY-5Y cell extract and 1,601 pg/mL in HeLa cell extract.

sELISA

Unknown

Sandwich ELISA - Human LC3A ELISA Kit (AB239432)

Example of human LC3A standard curve in 1X Cell Extraction Buffer PTR. The LC3A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.1 pg/mL

Range

39.06 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human LC3A ELISA Kit (ab239432) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LC3A protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human LC3A with 2.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]

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Recovery

[ { "sample": "Cell culture extracts", "range": "97 - 109 %", "average": "= 103" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab239432-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB239432-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB239432-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB239432-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB239432-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB239432-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB239432-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LC3A Capture Antibody", "number":"AB239432-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human LC3A Lyophilized Recombinant Protein", "number":"AB239432-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB239432-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB239432-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB239432-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LC3A Detector Antibody", "number":"AB239432-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein LC3A also known as Microtubule-associated proteins 1A/1B light chain 3A plays an important role in the autophagy process. It exists in two forms LC3-I and LC3-II with the latter being lipidated and integrated into autophagosomal membranes. LC3A has a molecular mass of approximately 17 kDa. Researchers observe its expression in various tissues but most highly in muscle liver and brain. It is often studied along with LC3B due to their similar roles in autophagy.

Biological function summary

LC3A participates in the formation of autophagosomes which are double-membraned vesicles that capture cellular waste for degradation. LC3A's conversion from LC3-I to LC3-II on the autophagosomal membrane signals the initiation of autophagy. It associates with proteins in the Atg family forming complexes that regulate the maturation of autophagosomes. The LC3 family including LC3A and LC3B acts as important components in the cell's ability to maintain homeostasis through autophagic activity.

Pathways

LC3A plays a vital part in the autophagy pathway and is also linked to the mTOR signaling pathway. Autophagy is a process that helps with the recycling of cellular components and mTOR signaling inhibits autophagy when nutrients are abundant. LC3A's interactions with other proteins such as p62 and ULK1 are essential in modulating these processes and ensuring cell survival under stress conditions. LC3A and LC3B's roles are integral in coordinating the cellular response to nutrient status and stress highlighting their importance in cellular regulation.

LC3A has significant connections to cancer and neurodegenerative diseases. Altered autophagic activity where LC3A takes part appears in various forms of cancer indicating its role in tumor progression and apoptosis regulation. Additionally abnormal LC3A function has implications in disorders such as Alzheimer's disease where autophagic dysfunction leads to neurodegeneration. In these contexts LC3A's relationship with proteins like p62 which accumulate in neurodegenerative diseases provides insight into pathological mechanisms and potential therapeutic targets.

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Product protocols

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Target data

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed : 20713600, PubMed : 24290141). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed : 20713600). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed : 31006537, PubMed : 31006538).

See full target information MAP1LC3A

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:7024 PubMed40016261

2025

NLRP3 overexpression exacerbated synovium tissue degeneration in juvenile collagen-induced arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Fater A Khadour,Younes A Khadour,Tao Xu

Cells 12: PubMed37174682

2023

Autophagy Receptor p62 Regulates SARS-CoV-2-Induced Inflammation in COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Verica Paunovic,Ljubica Vucicevic,Maja Misirkic Marjanovic,Vladimir Perovic,Biljana Ristic,Mihajlo Bosnjak,Milos Mandic,Danijela Stevanovic,Ljubica Harhaji-Trajkovic,Jovan Lalosevic,Milos Nikolic,Branka Bonaci-Nikolic,Vladimir Trajkovic

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human LC3A ELISA Kit

Sandwich ELISA - Human LC3A ELISA Kit (AB239432)

Sandwich ELISA - Human LC3A ELISA Kit (AB239432)

Sandwich ELISA - Human LC3A ELISA Kit (AB239432)

Sandwich ELISA - Human LC3A ELISA Kit (AB239432)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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Secondary Antibodies

Primary Antibodies

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