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Human LC3A ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human LC3A in Cell culture extracts samples.

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Images

Sandwich ELISA - Human LC3A ELISA Kit (AB239432), expandable thumbnail
  • Sandwich ELISA - Human LC3A ELISA Kit (AB239432), expandable thumbnail
  • Sandwich ELISA - Human LC3A ELISA Kit (AB239432), expandable thumbnail

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Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

39.06 - 2500 pg/mL

Assay time

1h 30m

Sensitivity

= 2.1 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20713600, PubMed:24290141). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20713600). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006537, PubMed:31006538).

Alternative names

What's included?

1 x 96 Tests
Components
10X Human LC3A Capture Antibody
1 x 600 µL
10X Human LC3A Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
50X Cell Extraction Enhancer Solution (ab193971)
1 x 1 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 10 mL
Antibody Diluent 4BI
1 x 6 mL
Human LC3A Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 12 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human LC3A ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human LC3A in Cell culture extracts samples.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

39.06 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 2.1 pg/mL

Precision

Intra assay

Sample

Cell Extract

n

8

mean

-

SD

-

C.V.

3.6

Inter assay

Sample

Cell Extract

n

3

mean

-

SD

-

C.V.

3.5

Recovery

Sample specific recovery

Sample type

Cell culture extracts

Average %

= 103

Range

97 - 109 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human LC3A ELISA Kit (ab239432) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LC3A protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human LC3A with 2.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein LC3A also known as Microtubule-associated proteins 1A/1B light chain 3A plays an important role in the autophagy process. It exists in two forms LC3-I and LC3-II with the latter being lipidated and integrated into autophagosomal membranes. LC3A has a molecular mass of approximately 17 kDa. Researchers observe its expression in various tissues but most highly in muscle liver and brain. It is often studied along with LC3B due to their similar roles in autophagy.

Biological function summary

LC3A participates in the formation of autophagosomes which are double-membraned vesicles that capture cellular waste for degradation. LC3A's conversion from LC3-I to LC3-II on the autophagosomal membrane signals the initiation of autophagy. It associates with proteins in the Atg family forming complexes that regulate the maturation of autophagosomes. The LC3 family including LC3A and LC3B acts as important components in the cell's ability to maintain homeostasis through autophagic activity.

Pathways

LC3A plays a vital part in the autophagy pathway and is also linked to the mTOR signaling pathway. Autophagy is a process that helps with the recycling of cellular components and mTOR signaling inhibits autophagy when nutrients are abundant. LC3A's interactions with other proteins such as p62 and ULK1 are essential in modulating these processes and ensuring cell survival under stress conditions. LC3A and LC3B's roles are integral in coordinating the cellular response to nutrient status and stress highlighting their importance in cellular regulation.

Associated diseases and disorders

LC3A has significant connections to cancer and neurodegenerative diseases. Altered autophagic activity where LC3A takes part appears in various forms of cancer indicating its role in tumor progression and apoptosis regulation. Additionally abnormal LC3A function has implications in disorders such as Alzheimer's disease where autophagic dysfunction leads to neurodegeneration. In these contexts LC3A's relationship with proteins like p62 which accumulate in neurodegenerative diseases provides insight into pathological mechanisms and potential therapeutic targets.

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3 product images

  • Sandwich ELISA - Human LC3A ELISA Kit (ab239432), expandable thumbnail

    Sandwich ELISA - Human LC3A ELISA Kit (ab239432)

    Example of human LC3A standard curve in 1X Cell Extraction Buffer PTR. The LC3A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human LC3A ELISA Kit (ab239432), expandable thumbnail

    Sandwich ELISA - Human LC3A ELISA Kit (ab239432)

    Interpolated concentrations of native LC3A in human SHSY-5Y cell extract based on a 30 μg/mL extract load, and HeLa cell extract based on a 500 μg/mL extract load. The concentrations of LC3A were measured in duplicate and interpolated from the LC3A standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LC3A concentration was determined to be 2,048 pg/mL in SHSY-5Y cell extract and 1,601 pg/mL in HeLa cell extract.

  • Sandwich ELISA - Human LC3A ELISA Kit (ab239432), expandable thumbnail

    Sandwich ELISA - Human LC3A ELISA Kit (ab239432)

    Comparison of LC3A in mock-treated and chloroquine-treated HeLa cell extracts. HeLa cells were cultured for 24 hours in the absence (mock) or presence of 50 mM chloroquine. The concentrations of LC3A were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the LC3A standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean LC3A concentration was determined to be 3.16 ng/mg in mock-treated HeLa cell extract, and 5.64 ng/mg in chloroquine-treated HeLa cell extract.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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