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AB313984

Human LCN1 ELISA Kit

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Human LCN1 ELISA Kit is a Sandwich ELISA for the measurement of Human LCN1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

VEGP, LCN1, Lipocalin-1, Tear lipocalin, Tear prealbumin, von Ebner gland protein, Tlc, TP, VEG protein

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Sandwich ELISA - Human LCN1 ELISA Kit (AB313984)
  • sELISA

Supplier Data

Sandwich ELISA - Human LCN1 ELISA Kit (AB313984)

Example of standard curve using ab313984. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.13 ng/mL

Range

0.13 - 30 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB232995

Anti-LCN1 antibody

Western blot - Anti-LCN1 antibody (AB232995)

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Proteins & Peptides

AB276407

Recombinant Human LCN1 protein (His tag)

SDS-PAGE - Recombinant Human LCN1 protein (His tag) (AB276407)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human LCN1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human LCN1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human LCN1 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "132 - 143 %", "average": "= 137.6" }, { "sample": "Plasma", "range": "124 - 145 %", "average": "= 136.4" }, { "sample": "Cell culture media", "range": "130 - 145 %", "average": "= 136.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313984-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313984-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin", "number":"AB313984-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313984-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313984-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313984-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human LCN1 Antibody-coated ELISA Plate", "number":"AB313984-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human LCN1 Protein Standard", "number":"AB313984-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human LCN1 Detection Antibody", "number":"AB313984-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"ELISA Microplate Bag", "number":"AB313984-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LCN1 also known as Lipocalin-1 or tear lipocalin is a secreted protein with a mass of approximately 21 kDa. It primarily localizes in human tears and other secretions including saliva. This protein belongs to the lipocalin family known for their small size and ability to bind hydrophobic molecules. Its key function includes transporting small lipophilic molecules such as fatty acids and retinoids contributing to ocular surface health.
Biological function summary

LCN1 plays an essential role in protecting the eye's surface. It binds and neutralizes bacteria-derived lipopolysaccharides which helps to control microbial growth on the eye surface. LCN1 may interact with other components present in tear film but it does not form a complex with them. The protein participates in transporting molecules across cell membranes and modulating the immune response at ocular and oral sites.

Pathways

LCN1 has a significant role in the defense mechanisms of the tear film. It engages in the immune response pathway helping to maintain homeostasis in the ocular surface microenvironment. Its binding capabilities are related to fatty acid transport pathways. LCN1 indirectly interacts with proteins related to lipid transport such as albumin due to its role in managing lipid interactions at epithelial surfaces.

LCN1 shows a connection to dry eye syndrome a condition resulting from insufficient tear production or quality leading to inflammation and damage to the eye surface. The protein also relates to Sjögren’s syndrome an autoimmune disorder affecting moisture-producing glands. These associations arise because LCN1's deficiency or dysfunction can contribute to the pathology observed in these disorders alongside proteins like lactoferrin which is also important in tear film stability and ocular health.
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Product protocols

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Target data

Could play a role in taste reception. Could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. Exhibits an extremely wide ligand pocket.
See full target information LCN1
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Product promise

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