Human LDLR ELISA Kit
- Recombinant
- SimpleStep
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Human LDLR ELISA Kit is a single-wash 90-min SimpleStep Sandwich ELISA used to quantify Human LDLR with a sensitivity of 55.8 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric sandwich ELISA - 450 nm readout, works on any plate reader
- Validated on a broad range of sample types including urine, serum, plasma, cell culture supernatant, cell lysate
- Need to design your own assay: we also provide the conjugation-ready antibody pair
View Alternative Names
Low-density lipoprotein receptor, LDL receptor, LDLR
- ELISA
Supplier Data
ELISA - Human LDLR ELISA Kit (AB270212)
- sELISA
Supplier Data
Sandwich ELISA - Human LDLR ELISA Kit (AB270212)
Interpolated concentrations of native LDLR in HepG2 cell extract based on a 200 μg/mL extract load.
The concentrations of LDLR were measured in duplicate and interpolated from the LDLR standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LDLR concentration was determined to be 11.8 ng/mL in sample.
- sELISA
Supplier Data
Sandwich ELISA - Human LDLR ELISA Kit (AB270212)
Example of human LDLR standard curve in Sample Diluent NS. as described in Section 10.
The LDLR standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human LDLR ELISA Kit (AB270212)
Interpolated concentrations of native LDLR in human serum and plasma samples.
The concentrations of LDLR were measured in duplicates, interpolated from the LDLR standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LDLR concentration was determined to be 34.6 ng/mL in serum, 41.6 ng/mL in plasma (citrate) and 24.9 ng/mL in plasma (EDTA), and 31.4 ng/mL in plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human LDLR ELISA Kit (AB270212)
Interpolated concentrations of native LDLR in human urine and cell culture supernatant samples.
The concentrations of LDLR were measured in duplicates, interpolated from the LDLR standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 2% and HepG2 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LDLR concentration was determined to be 223.5 ng/mL in urine and 28.0 ng/mL in HepG2 cell culture supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Human LDLR ELISA Kit (AB270212)
Serum from nine individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LDLR concentration was determined to be 65 ng/mL with a range of 38 – 115 ng/mL.
Complementary Products
Reactivity data
Product details
Human LDLR ELISA Kit ab270212 is a rapid single-wash 90-min ELISA kit to measure Human LDLR in cell culture supernatant, cell lysates, urine, serum and plasma.
How the assay works
Human LDLR SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant human alpha 1 Antitrypsin protein in serum and plasma. Normal human urine and saliva samples tested negative for this kit. Cell culture supernatant, milk, CSF, cell extract, and tissue extract samples have not been tested with this kit.
Human alpha 1 Anti-chymotrypsin protein and human PAI protein were prepared at 2,000 ng/mL and 1,000 ng/mL respectively and assayed for cross reactivity. No cross-reactivity was observed.
Human alpha 1 Anti-chymotrypsin protein and human PAI protein were prepared at 2,000 ng/mL and 1,000 ng/mL respectively and assayed for and tested for interference. No interference with was observed.
This immunoassay is calibrated against a highly purified human alpha 1 Antitrypsin. The NIBSC/WHO unclassified purified human alpha 1 Antitrypsin preparation 05/162 was evaluated in this kit. The dose response curve of the unclassified standard alpha 1 Antitrypsin parallels the SimpleStep standard curve. To convert sample values obtained with the SimpleStep Human alpha 1 Antitrypsin kit to approximate NIBSC 05/162 units, use the equation below.
NIBSC (05/162) approximate value (ng/mL) = 0.3649 x SimpleStep Human alpha 1 Antitrypsin value (ng/mL).
Human LDLR ELISA Kit ab270212 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human LDLR Antibody Pair - BSA and Azide free ab253310
- Anti-LDL Receptor antibody [EPR22296-56] - BSA and Azide free (Capture) ab272291
- Anti-LDL Receptor antibody [EPR22296-103] - BSA and Azide free (Detector) ab272292
Related and recommended products
Human LDLR ELISA Kit ab270212 is commonly used to study cardiovascular diseases and lipid metabolism.
Other ELISA kits to measure lipid metabolism are:
- Human PCSK9 ELISA kit ab190806
- Human HDL ELISA kit ab125961
Low-density lipoprotein receptor (LDLR) is well known receptor found clustered in clathrin-coated pits. LDLR mediates the endocytosis of low-density lipoprotein (LDL) and maintains the plasma level of LDL. Studies have shown LDLR as a primary method of entry for vesicular stomatitis virus in humans. Also reported other viruses such as hepatitis C entering the cells indirectly by association with LDL via LDLR-mediated endocytosis.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The LDL receptor interacts with LDL particles to regulate cholesterol levels in the body. It is part of a cell surface complex that recognizes and binds to apolipoprotein B-100 or apolipoprotein E present on LDL. This interaction initiates internalization of LDL leading to its degradation in lysosomes where cholesterol can be released and used by the cell. Mutations in the gene encoding LDLR can lead to inefficient cholesterol uptake influencing various metabolic processes.
Pathways
LDL receptor activities are integral to lipid metabolism and cholesterol homeostasis. Two important biological pathways that involve LDLR include the cholesterol biosynthesis pathway and the lipoprotein clearance pathway. Within these pathways LDLR collaborates closely with proteins like PCSK9 which modulates its expression and degradation and HMG-CoA reductase an important enzyme in cholesterol synthesis to balance cholesterol levels in the body.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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