AB269554

Human LECT2 ELISA Kit

Recombinant
SimpleStep
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Human LECT2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human LECT2 with a sensitivity of 0.706 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Leukocyte cell-derived chemotaxin-2, LECT-2, hLECT2, LECT2

4 Images

Sandwich ELISA - Human LECT2 ELISA Kit (AB269554)

sELISA

Supplier Data

Sandwich ELISA - Human LECT2 ELISA Kit (AB269554)

Example of human LECT2 standard curve in Sample Diluent NS.

The LECT2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human LECT2 ELISA Kit (AB269554)

Interpolated concentrations of native LECT2 in human lung extract based on a 1,000 μg/mL extract load.

The concentrations of LECT2 were measured in duplicate and interpolated from the LECT2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LECT2 concentration was determined to be 8.8 ng/mL in lung extract.

sELISA

Supplier Data

Sandwich ELISA - Human LECT2 ELISA Kit (AB269554)

Serum from ten individual healthy human female donors were measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LECT2 concentration was determined to be 51 ng/mL with a range of 20 - 125 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human LECT2 ELISA Kit (AB269554)

Interpolated concentrations of native LECT2 in human serum and plasma samples.

The concentrations of LECT2 were measured in duplicates, interpolated from the LECT2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma-citrate 25%, plasma-EDTA 25%, and plasma-heparin 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LECT2 concentration was determined to be 28.4 ng/mL in serum, 22.5 ng/mL in plasma-citrate, 32.5 ng/mL in plasma-EDTA, and 13.0 ng/mL in plasma-heparin.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.706 ng/mL

Range

1.56 - 100 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human LECT2 ELISA Kit (ab269554) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LECT2 protein in hep plasma, serum, cit plasma, tissue extracts, and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human LECT2 with 0.706 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Leukocyte cell derived chemotaxin 2 (LECT2) is a highly conserved protein that functions with major development in liver regeneration, immune modulation, bone growth, neuronal development, cancer, and several more in pathophysiology. Studies have shown LECT2 to be a positive regulator of chondrocyte proliferation. Moreover, the liver is considered to be the source of LECT2 due to exceptionally high levels of mRNA. However, expression in these cells is extremely low or undetectable suggesting that hepatocytes secrete LECT2 instantly after production.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" } ]

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Recovery

[ { "sample": "Serum", "range": "91 - 96 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "113 - 118 %", "average": "= 115" }, { "sample": "Tissue Extracts", "range": "120 - 120 %", "average": "= 120" }, { "sample": "Heparin Plasma", "range": "99 - 103 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "111 - 117 %", "average": "= 114" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target 'LECT2' also known as leukocyte cell-derived chemotaxin 2 is a 16 kDa protein. It plays a role in immune response and signaling by acting as a chemotactic factor for neutrophils. LECT2 is a secreted protein primarily expressed in the liver and found in circulation in the bloodstream. It has been associated with acute-phase response and inflammation.

Biological function summary

LECT2 contributes to tissue regeneration liver function and modulation of immune responses. It is not known to be part of a larger protein complex but interacts with multiple receptor types on cell surfaces. LECT2 can inhibit mitogen-activated protein kinase (MAPK) pathway by binding to specific receptors affecting cell proliferation and survival. It also plays a role in metabolic processes.

Pathways

The LECT2 protein influences the MAPK and JNK pathways impacting cellular stress responses and inflammatory signaling. Its interactions with other proteins involve MAPK family members like p38 and ERK. These pathways regulate various cellular activities such as proliferation apoptosis and cytokine production positioning LECT2 as a modulator of cellular signaling networks.

LECT2 has strong connections to liver-related conditions such as fatty liver disease and liver fibrosis. It also associates with conditions like amyloid A kidney amyloidosis. In these diseases LECT2 interacts with inflammatory cytokines like interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) which play roles in inflammation and fibrosis. These interactions can alter disease outcomes and affect therapeutic strategies targeting these inflammatory pathways.

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Product protocols

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Target data

Has a neutrophil chemotactic activity. Also a positive regulator of chondrocyte proliferation (PubMed : 9524238). Does not show metalloendopeptidase activity (PubMed : 27334921).

See full target information Leukocyte cell-derived chemotaxin-2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human LECT2 ELISA Kit

Sandwich ELISA - Human LECT2 ELISA Kit (AB269554)

Sandwich ELISA - Human LECT2 ELISA Kit (AB269554)

Sandwich ELISA - Human LECT2 ELISA Kit (AB269554)

Sandwich ELISA - Human LECT2 ELISA Kit (AB269554)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise