AB327308

Human LECT2 ELISA Kit, Chemiluminescent

SimpleStep Ignite™ ELISA
Recombinant
What is this?

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Human LECT2 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human LECT2 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.14 - 300 ng/ml with a sensitivity of 0.07 ng/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

5 Images

Sandwich ELISA - Human LECT2 ELISA Kit, Chemiluminescent (AB327308)

sELISA

Supplier Data

Sandwich ELISA - Human LECT2 ELISA Kit, Chemiluminescent (AB327308)

Serum from ten individual healthy human female donors were measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean LECT2 concentration was determined to be 51 ng/mL with a range of 20 - 125 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human LECT2 ELISA Kit, Chemiluminescent (AB327308)

Interpolated concentrations of native LECT2 in human lung extract based on a 1000 µg/mL extract load.

The concentrations of LECT2 were measured in duplicate and interpolated from the LECT2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean LECT2 concentration was determined to be 8.8 ng/mL in lung extract.

sELISA

Supplier Data

Sandwich ELISA - Human LECT2 ELISA Kit, Chemiluminescent (AB327308)

Interpolated concentrations of native LECT2 in human serum and plasma samples.

The concentrations of LECT2 were measured in duplicates interpolated from the LECT2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25% plasma-citrate 25% plasma-EDTA 25% and plasma-heparin 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean LECT2 concentration was determined to be 28.4 ng/mL in serum 22.5 ng/mL in plasma-citrate 32.5 ng/mL in plasma-EDTA and 13.0 ng/mL in plasma-heparin.

sELISA

Lab

Sandwich ELISA - Human LECT2 ELISA Kit, Chemiluminescent (AB327308)

Standard curve comparison between the Chemiluminescent Human LECT2 SimpleStep ELISA and colorimetric Human LECT2 SimpleStep ELISA (ab269554) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human LECT2 ELISA Kit, Chemiluminescent (AB327308)

Dynamic range comparison between the Chemiluminescent Human LECT2 SimpleStep ELISA, colorimetric Human LECT2 SimpleStep ELISA (ab269554), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture media

Reacts with

Human, Monkey

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.07 ng/mL

Range

0.14 - 300 ng/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human LECT2 ELISA Kit, Chemiluminescent (ab327308) is a rapid single-wash sandwich 55-min ELISA to measure Human LECT2 in Serum, EDTA Plasma, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture media with a sensitivity of 0.07 ng/mL.

How the assay works

Human LECT2 ELISA Kit, Chemiluminescent (ab327308) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human LECT2 (ab327308) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Human LECT2 antibody [EPR22049-227] - BSA and Aside free (Capture) ab270338
- Anti-Human LECT2 antibody [EPR22049-87] - BSA and Aside free (Detector) ab270339

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.4" } ]

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Recovery

[ { "sample": "Serum", "range": "91 - 96 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "113 - 118 %", "average": "= 115" }, { "sample": "Tissue Extracts", "range": "120 - 120 %", "average": "= 120" }, { "sample": "Heparin Plasma", "range": "99 - 103 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "111 - 117 %", "average": "= 114" }, { "sample": "Cell culture media", "range": "81 - 85 %", "average": "= 83" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Has a neutrophil chemotactic activity. Also a positive regulator of chondrocyte proliferation (PubMed : 9524238). Does not show metalloendopeptidase activity (PubMed : 27334921).

See full target information Leukocyte cell-derived chemotaxin-2

Alternative Names

Leukocyte cell-derived chemotaxin-2, LECT-2, hLECT2, LECT2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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