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AB313982

Human LEFTY2 ELISA Kit

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Human LEFTY2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human LEFTY2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

EBAF, LEFTA, LEFTYA, TGFB4, PSEC0024, LEFTY2, Left-right determination factor 2, Endometrial bleeding-associated factor, Left-right determination factor A, Protein lefty-2, Protein lefty-A, Transforming growth factor beta-4, TGF-beta-4

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Sandwich ELISA - Human LEFTY2 ELISA Kit (AB313982)
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Supplier Data

Sandwich ELISA - Human LEFTY2 ELISA Kit (AB313982)

Example of standard curve using ab313982. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.82 ng/mL

Range

0.82 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human LEFTY2 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human LEFTY2 serum (human LEFTY2 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human LEFTY2 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "88 - 123 %", "average": "= 104.2" }, { "sample": "Plasma", "range": "72 - 110 %", "average": "= 93.52" }, { "sample": "Cell culture media", "range": "110 - 148 %", "average": "= 128.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313982-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313982-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313982-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313982-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313982-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human LEFTY2 Protein Standard", "number":"AB313982-CMP11", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB313982-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"700X HRP-Streptavidin", "number":"AB313982-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human LEFTY2 Detection Antibody", "number":"AB313982-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human LEFTY2 Antibody-coated ELISA Plate", "number":"AB313982-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LEFTY2 also known as Left-Right Determination Factor 2 is a member of the transforming growth factor beta (TGF-β) superfamily. This protein has a molecular mass of approximately 38 kDa. LEFTY2 is expressed mainly in visceral endoderm and left side mesoderm tissues. The gene encoding LEFTY2 is found within the human chromosome 1. This protein plays a mechanical role in the regulation of cellular signaling and developmental processes.
Biological function summary

LEFTY2 functions as an important regulator in ensuring proper left-right asymmetry during embryonic development. It is a monomeric protein that is not part of a larger complex. LEFTY2 contributes to the modulation of Nodal signaling inhibiting excessive activity that can disrupt normal development. This modulation ensures the establishment of asymmetric organ positioning and plays an important role in maintaining developmental balance in the body.

Pathways

LEFTY2 integrates into the Nodal signaling pathway and the Activin signaling pathway. The interaction with Nodal proteins narrows down Nodal signaling ensuring the accurate development of the left-right axis. LEFTY2 also relates functionally and antagonistically to other proteins like Nodal and Activin A balancing their signaling actions to regulate developmental processes.

LEFTY2 has a connection with asymmetry-related conditions such as situs inversus where organ placement within the body is mirrored from normal positions. Additionally research links irregular LEFTY2 expression to some cancer types including ovarian cancer. Through these disorders LEFTY2 interacts with proteins like Nodal which plays a role in cancer cell proliferation by influencing the signaling pathways that govern cellular growth and differentiation.
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Product protocols

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Target data

Required for left-right (L-R) asymmetry determination of organ systems in mammals. May play a role in endometrial bleeding.
See full target information LEFTY2
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB34593

Anti-LEFTY2 antibody [7C5G1H6H10]

Western blot - Anti-LEFTY2 antibody [7C5G1H6H10] (AB34593)

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Proteins & Peptides

AB276234

Recombinant Human LEFTY2 protein (Fc Chimera)

SDS-PAGE - Recombinant Human LEFTY2 protein (Fc Chimera) (AB276234)

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