AB282876

Human Leptin Receptor ELISA Kit

Recombinant
SimpleStep
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Human Leptin Receptor ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Leptin Receptor with a sensitivity of 20.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD295, DB, OBR, LEPR, Leptin receptor, LEP-R, HuB219, OB receptor, OB-R

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Sandwich ELISA - Human Leptin Receptor ELISA Kit (AB282876)

sELISA

Supplier Data

Sandwich ELISA - Human Leptin Receptor ELISA Kit (AB282876)

Interpolated concentrations of native Leptin Receptor in human serum and plasma samples.

The concentrations of Leptin Receptor were measured in duplicates, interpolated from the Leptin Receptor standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 25, plasma (citrate) 1 : 25, plasma (EDTA) 1 : 25, and plasma (heparin) 1 : 25. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Leptin Receptor concentration was determined to be 92.6 ng/mL in serum, 93.4 ng/mL in plasma (citrate), 78.9 ng/mL in plasma (EDTA) 79.5 ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human Leptin Receptor ELISA Kit (AB282876)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Leptin Receptor concentration was determined to be 92.2 ng/mL with a range of 53.1 – 170.3 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Leptin Receptor ELISA Kit (AB282876)

Human Leptin Receptor curve comparison.

Standard Curve comparison between Human Leptin Receptor SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human Leptin Receptor ELISA Kit (AB282876)

Interpolated concentrations of native Leptin Receptor in human K562 cell extract sample based on a 100 μg/mL extract load.

The concentrations of Leptin Receptor were measured in duplicate and interpolated from the Leptin Receptor standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Leptin Receptor concentration was determined to be 2,227 pg/mL in K562 extract sample.

sELISA

Supplier Data

Sandwich ELISA - Human Leptin Receptor ELISA Kit (AB282876)

Example of human Leptin Receptor standard curve in Sample Diluent NS.

The Leptin Receptor standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 20.4 pg/mL

Range

93.75 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Leptin Receptor ELISA kit (ab282876) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Leptin Receptor protein in human serum, plasma, cell culture supernatant, and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Leptin Receptor with 20.4 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Leptin receptor, encoded by the LEPR gene, is a type I cytokine receptor and well known to play a role in ligand binding with a fat cell specific hormone leptin. The leptin receptor/leptin interaction in the hypothalamus plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Studies has shown variations in the leptin receptor have been associated with obesity.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.98" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]

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Recovery

[ { "sample": "Serum", "range": "90 - 105 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "102 - 108 %", "average": "= 105" }, { "sample": "Cell culture media", "range": "86 - 93 %", "average": "= 88" }, { "sample": "Heparin Plasma", "range": "90 - 105 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "89 - 102 %", "average": "= 98" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Leptin Receptor commonly known as LEPR or OB-R is a protein that plays an important role in energy homeostasis and weight regulation. Mechanically it functions as a receptor for leptin the hormone predominantly produced by adipocytes. Leptin binding triggers activation of intracellular signaling pathways. The molecular weight of the Leptin Receptor can vary as it exists in several isoforms due to alternative splicing. It is prominently expressed in the hypothalamus and other tissues involved in energy regulation such as the liver and skeletal muscle.

Biological function summary

The Leptin Receptor acts as a gateway for leptin to exert its effects on the central nervous system and peripheral tissues. When leptin binds to LEPR it facilitates the formation of receptor complexes that engage downstream signaling molecules like STAT3 and MAPK. This results in regulation of appetite and energy expenditure. LEPR is not part of a complex with other receptors but it interacts with intracellular signaling proteins to mediate leptin's physiological effects.

Pathways

The Leptin Receptor is integral to the JAK-STAT signaling pathway and the MAPK pathway both of which are important in maintaining energy balance. LEPR activates JAK2 upon ligand binding leading to phosphorylation events that propagate the signal. It is closely associated with other proteins like SOCS3 which acts as a feedback inhibitor of leptin signaling playing a balancing role in the pathways mentioned.

The Leptin Receptor has significant associations with obesity and certain types of diabetes. LEPR mutations or deficiencies can lead to hyperphagia leading to severe obesity due to the disruption of normal leptin signaling. Additionally interactions between leptin and insulin signaling pathways can impact the development of Type 2 diabetes. In these conditions the Leptin Receptor often interacts with the insulin receptor highlighting its role in the complex network of metabolic regulation.

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Product protocols

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Target data

Receptor for hormone LEP/leptin (Probable) (PubMed : 22405007). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones (By similarity) (PubMed : 9537324). In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity (PubMed : 12504075, PubMed : 25060689, PubMed : 8805376). Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis. Involved in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus. Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T -ells. Leptin increases Th1 and suppresses Th2 cytokine production (By similarity).. Isoform A. May transport LEP across the blood-brain barrier. Binds LEP and mediates LEP endocytosis. Does not induce phosphorylation of and activate STAT3.. Isoform E. Antagonizes Isoform A and isoform B-mediated LEP binding and endocytosis.

See full target information LEPR

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websiteProtocolBooklet

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human Leptin Receptor ELISA Kit

Sandwich ELISA - Human Leptin Receptor ELISA Kit (AB282876)

Sandwich ELISA - Human Leptin Receptor ELISA Kit (AB282876)

Sandwich ELISA - Human Leptin Receptor ELISA Kit (AB282876)

Sandwich ELISA - Human Leptin Receptor ELISA Kit (AB282876)

Sandwich ELISA - Human Leptin Receptor ELISA Kit (AB282876)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise