JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB313987

Human Leukotriene A4 hydrolase/LTA4H ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Leukotriene A4 hydrolase/LTA4H ELISA Kit is a Sandwich ELISA for the measurement of Human Leukotriene A4 hydrolase/LTA4H in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

LTA4, LTA4H, Leukotriene A-4 hydrolase, LTA-4 hydrolase, Leukotriene A(4) hydrolase, Tripeptide aminopeptidase LTA4H

1 Images
Sandwich ELISA - Human Leukotriene A4 hydrolase/LTA4H ELISA Kit (AB313987)
  • sELISA

Supplier Data

Sandwich ELISA - Human Leukotriene A4 hydrolase/LTA4H ELISA Kit (AB313987)

Example of standard curve using ab313987. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.1 ng/mL

Range

2.1 - 500 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Primary Antibodies

AB133512

Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5713]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5713] (AB133512)

  • 0
  • 0
Proteins & Peptides

AB201716

Recombinant Human Leukotriene A4 hydrolase/LTA4H protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Leukotriene A4 hydrolase/LTA4H protein (His tag N-Terminus) (AB201716)

  • 0
  • 0
Cell Lines & Lysates

AB266467

Human LTA4H (Leukotriene A4 hydrolase) knockout HEK-293T cell line

Advanced Validation

Western blot - Human LTA4H (Leukotriene A4 hydrolase) knockout HEK-293T cell line (AB266467)

  • 0
  • 0
Cell Lines & Lysates

AB258034

Human LTA4H (Leukotriene A4 hydrolase) knockout HEK-293T cell lysate

Western blot - Human LTA4H (Leukotriene A4 hydrolase) knockout HEK-293T cell lysate (AB258034)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Leukotriene A4 hydrolase/LTA4H ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human LTA4H in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human LTA4H coated on a 96-well plate.
style
left-column

Recovery

[ { "sample": "Serum", "range": "73 - 91 %", "average": "= 79.37" }, { "sample": "Plasma", "range": "69 - 131 %", "average": "= 88.39" }, { "sample": "Cell culture media", "range": "78 - 104 %", "average": "= 91.55" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313987-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Lyophilized Human Leukotriene A4 hydrolase/LTA4H Protein Standard", "number":"AB313987-CMP11", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313987-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB313987-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313987-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Leukotriene A4 hydrolase/LTA4H Antibody-coated ELISA Plate", "number":"AB313987-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313987-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313987-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB313987-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Leukotriene A4 hydrolase/LTA4H Detection Antibody", "number":"AB313987-CMP10", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Leukotriene A4 hydrolase often called LTA4H is an enzyme that converts leukotriene A4 into leukotriene B4 an important mediator in inflammation. It has a molecular mass of approximately 69 kDa and functions as a zinc metalloprotease. Expression of LTA4H is widespread detected in various tissues including the lung liver and kidney. This enzyme plays an essential role in modulating inflammatory responses due to its ability to catalyze the transformation of leukotriene A4.
Biological function summary

Leukotriene A4 hydrolase (LTA4H) transforms leukotriene A4 an unstable epoxide into leukotriene B4 a potent chemoattractant. The enzyme does not function within a multi-protein complex but interacts with small molecules during leukotriene biosynthesis. Leukotriene B4 produced by LTA4H recruits neutrophils to sites of inflammation enhancing immune defense. While its primary function is promoting inflammation this activity also regulates the resolution phase by facilitating tissue repair and return to homeostasis.

Pathways

Leukotriene A4 hydrolase plays a role in the arachidonic acid cascade an important biological pathway for synthesizing eicosanoids. This pathway involves several steps where arachidonic acid releases from cell membrane phospholipids and converts to intermediate leukotriene A4 by 5-lipoxygenase. LTA4H then converts leukotriene A4 into leukotriene B4 which orchestrates inflammatory responses. LTA4H connects to proteins like 5-lipoxygenase which also acts within this cascade.

Leukotriene A4 hydrolase associates with inflammatory diseases such as asthma and inflammatory bowel disease. In asthma the overproduction of leukotriene B4 can exacerbate airway inflammation and hyperresponsiveness. Here leukotrienes interact with receptors on effector cells which contributes to the pathology of asthma. Inflammatory bowel disease showcases a similar link where increased levels of leukotriene B4 potentiate intestinal inflammation. Effective management of these conditions may involve targeting leukotriene pathways specifically inhibiting the activity of LTA4H to ameliorate symptoms.
style
left-column

Product protocols

style
left-column

Target data

Bifunctional zinc metalloenzyme that comprises both epoxide hydrolase (EH) and aminopeptidase activities. Acts as an epoxide hydrolase to catalyze the conversion of LTA4 to the pro-inflammatory mediator leukotriene B4 (LTB4) (PubMed : 11917124, PubMed : 12207002, PubMed : 15078870, PubMed : 18804029, PubMed : 1897988, PubMed : 1975494, PubMed : 2244921, PubMed : 2996528). Can utilize LTA5 less effectively as a substrate than LTA4, and produce LTB5 (PubMed : 2996528). Also has aminopeptidase activity, with high affinity for N-terminal arginines of various synthetic tripeptides (PubMed : 18804029, PubMed : 20813919). In addition to its pro-inflammatory EH activity, may also counteract inflammation by its aminopeptidase activity, which inactivates by cleavage another neutrophil attractant, the tripeptide Pro-Gly-Pro (PGP), a bioactive fragment of collagen generated by the action of matrix metalloproteinase-9 (MMP9) and prolylendopeptidase (PREPL) (PubMed : 20813919, PubMed : 24591641). Involved also in the biosynthesis of resolvin E1 and 18S-resolvin E1 from eicosapentaenoic acid, two lipid mediators that show potent anti-inflammatory and pro-resolving actions (PubMed : 21206090).
See full target information LTA4H
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com