Human LHB ELISA Kit is a single-wash 90-min Simplestep used to quantify Human LHB with a sensitivity of 21.227 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 42.969 - 2750 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 21.227 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
Alternative names
Lutropin subunit beta, Lutropin beta chain, Luteinizing hormone subunit beta, LH-B, LSH-B, LSH-beta, LHB
What's included?
Recommended products
Human LHB ELISA Kit is a single-wash 90-min Simplestep used to quantify Human LHB with a sensitivity of 21.227 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 42.969 - 2750 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 21.227 pg/mL
- General recovery
- 105%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 3.2 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 4.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture media | Average % = 111 | Range 108 - 115 % |
Sample type Citrate plasma | Average % = 90 | Range 80 - 101 % |
Sample type EDTA Plasma | Average % = 93 | Range 83 - 104 % |
Sample type Heparin Plasma | Average % = 92 | Range 89 - 94 % |
Sample type Serum | Average % = 105 | Range 92 - 119 % |
Sample type Urine | Average % = 86 | Range 84 - 89 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human LHB SimpleStep ELISA® kit (ab303746) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human LHB protein in serum, plasma (EDTA), plasma (hep), plasma (cit), urine and cell culture media. Quantitate Human LHB with 21.227 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LHB also known as luteinizing hormone beta or beta LH is a subunit of luteinizing hormone (LH). It plays an important mechanical role in gonadal function. LHB shares a common alpha subunit with other glycoprotein hormones while the beta subunit which is unique to each hormone confers biological specificity. LHB contributes to the dimeric structure of LH giving it a molecular mass around 30 kDa. This protein is expressed mainly in the anterior pituitary gland where it interacts with cells that influence reproductive processes.
- Biological function summary
LHB binds with the alpha subunit to form the active LH hormone which importantly stimulates the ovaries in females and testes in males. In females LH promotes the production of estrogen and progesterone facilitating ovulation and menstrual cycles. In males it stimulates Leydig cells leading to testosterone production. LH hormone functions as part of a complex with its receptor a G protein-coupled complex influencing the reproductive system's normal operations.
- Pathways
The LHB is central to the hypothalamic-pituitary-gonadal axis a feedback loop that regulates reproductive physiology. Within this axis gonadotropin-releasing hormone (GnRH) from the hypothalamus stimulates LHB production in concert with follicle-stimulating hormone (FSH). These hormones together regulate the development and function of the gonads. LHB's interaction with LH receptors on gonadal tissues signifies its operational involvement in the steroidogenesis and gametogenesis pathways.
- Associated diseases and disorders
Abnormalities in LHB expression associate with conditions such as polycystic ovary syndrome (PCOS) and hypogonadism. In PCOS altered LHB levels lead to hormonal imbalances affecting fertility. Hypogonadism may result from insufficient LHB affecting sexual development and function. LHB's influence on testosterone and estrogen levels links it closely with disorders characterized by hormonal deficiencies or excesses tying it to complications involving the HPG axis.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Sandwich ELISA - Human LHB ELISA Kit (ab303746)
Serum of 4 individual healthy human female donors (donors 1-4) and 4 individual healthy human male donors (donors 5-8) was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LHB concentration was determined to be 4985 pg/mL with a range of 717 - 14998 pg/mL for female donors. The mean LHB concentration was determined to be 651 pg/mL with a range of 403 - 936 pg/mL for male donors.
Sandwich ELISA - Human LHB ELISA Kit (ab303746)
Example of human LHB standard curve in Sample Diluent NS.
Example of human LHB standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human LHB ELISA Kit (ab303746)
Interpolated concentrations of human LHB in serum, plasma (EDTA), plasma (citrate), plasma (heparin), and urine.
Interpolated concentration of native LHB was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (citrate) 50%, plasma (heparin) 25%, and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com