AB242228

Human LIF ELISA Kit

Recombinant
SimpleStep
What is this?

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(2 Publications)

Human LIF ELISA Kit is a single-wash 90-min Simplestep used to quantify Human LIF with a sensitivity of 1.469 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

HILDA, LIF, Leukemia inhibitory factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor, D factor, MLPLI

6 Images

ELISA

Supplier Data

ELISA - Human LIF ELISA Kit (AB242228)

sELISA

Supplier Data

Sandwich ELISA - Human LIF ELISA Kit (AB242228)

Human LIF Standard Curve Comparison.

Standard Curve comparison between human LIF SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Unknown

Sandwich ELISA - Human LIF ELISA Kit (AB242228)

Interpolated concentrations of native LIF in human cell culture supernatant samples. The concentrations of LIF were measured in duplicates, interpolated from the LIF standard curves and corrected for sample dilution. Undiluted samples are 1.5% PHA-M treated PBMC cell culture supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIF concentration was determined to be 8336.44 pg/mL in 1.5% PHA-M treated PBMC cell culture supernatant.

sELISA

Unknown

Sandwich ELISA - Human LIF ELISA Kit (AB242228)

Example of human LIF standard curve in Sample Diluent NS. The LIF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human LIF ELISA Kit (AB242228)

Example of human LIF standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human LIF ELISA Kit (AB242228)

Interpolated concentration of native LIF was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 16 stimulated human PBMC supernatant (1.5% PHA-M for 46 hrs). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.469 pg/mL

Range

23.44 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human LIF ELISA Kit ab242228 is a rapid single-wash 90-min Sandwich ELISA to measure Human LIF in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 1.469 pg/mL.

How the assay works

Human LIF SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human LIF ELISA Kit ab242228 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-LIF antibody [EPR21949-30] - BSA and Azide free (Capture) ab259460
- Anti-LIF antibody [EPR21949-92] - BSA and Azide free (Detector) ab259460

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Media", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" }, { "reproducibilityType": "Intra", "sample": "Cell Media", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "99 - 111 %", "average": "= 102" }, { "sample": "Serum", "range": "85 - 93 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "87 - 92 %", "average": "= 89" }, { "sample": "Heparin Plasma", "range": "87 - 92 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "89 - 91 %", "average": "= 89" } ]

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What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab242228-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB242228-CMP03" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB242228-CMP11" }, { "size":"1 x 600 µL", "name":"10X Human LIF Detector Antibody", "number":"AB242228-CMP07" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB242228-CMP04" }, { "size":"1 x 600 µL", "name":"10X Human LIF Capture Antibody", "number":"AB242228-CMP06" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB242228-CMP10" }, { "size":"2 x 1 Vial", "name":"Human LIF Lyophilized Recombinant Protein", "number":"AB242228-CMP08" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB242228-CMP12" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB242228-CMP05" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB242228-CMP09" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB242228-CMP02" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab242228-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB242228-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB242228-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB242228-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB242228-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB242228-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB242228-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human LIF Lyophilized Recombinant Protein", "number":"AB242228-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LIF Capture Antibody", "number":"AB242228-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LIF Detector Antibody", "number":"AB242228-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB242228-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Leukemia Inhibitory Factor (LIF) also known as LIF protein plays a critical mechanical role in cell communication and survival. It is a glycoprotein with a molecular weight (LIF MW) generally around 19 kilodaltons. In various tissues particularly in the mouse LIF expression occurs in the bone marrow muscle and notably the nervous system. Besides its presence in normal tissues it shows up in embryonic stem cells where it maintains their pluripotency in culture conditions.

Biological function summary

LIF influences cell differentiation growth and inflammatory response. As part of the interleukin-6 cytokine family LIF exerts its effects through a receptor complex involving LIFR (LIF receptor) and GP130. The LIF protein's signaling controls processes like neuronal cell differentiation and bone formation. It impacts hematopoietic cells and supports embryonic implantation and placental development by preparing the uterine environment.

Pathways

The LIF protein is essential in the JAK/STAT signaling pathway which regulates gene expression vital for survival and proliferation. It also interacts with the MAPK/ERK pathway ensuring diverse cellular responses. These pathways link LIF to related proteins like STAT3 and ERK1/2 which mediate further downstream effects. The interplay between these signaling cascades highlights LIF's role in managing cell survival and proliferation in various contexts.

LIF contributes to physiological and pathological states. In cancer such as leukemia LIF influences tumor growth and cancer cell survival. The interaction with other cytokines like Interleukin-6 enhances its effect in oncogenic processes. In inflammatory diseases like rheumatoid arthritis LIF's role relates to inflammation and joint destruction. Understanding its interaction with inflammatory mediators can aid in targeted therapeutic strategies.

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Product protocols

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Target data

LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

See full target information LIF

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:360 PubMed40319033

2025

Comprehensive secretome profiling and CRISPR screen identifies SFRP1 as a key inhibitor of epidermal progenitor proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Binbin Cheng,Shaohong Isaac Peng,Yunlong Y Jia,Elton Tong,Scott X Atwood,Bryan K Sun

Cell death & disease 15:218 PubMed38490994

2024

TGF-β-driven LIF expression influences neutrophil extracellular traps (NETs) and contributes to peritoneal metastasis in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fangbin Zhang,Yan Yan,Xinguang Cao,Changqing Guo,Ke Wang,Shuai Lv

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human LIF ELISA Kit

ELISA - Human LIF ELISA Kit (AB242228)

Sandwich ELISA - Human LIF ELISA Kit (AB242228)

Sandwich ELISA - Human LIF ELISA Kit (AB242228)

Sandwich ELISA - Human LIF ELISA Kit (AB242228)

Sandwich ELISA - Human LIF ELISA Kit (AB242228)

Sandwich ELISA - Human LIF ELISA Kit (AB242228)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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