Human Lipocalin-2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Lipocalin-2 with a sensitivity of 14.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Publications
Filter by
- Journal of inflammation research 14:7035-70512021OLFM4 Regulates Lung Epithelial Cell Function in Sepsis-Associated ARDS/ALI via LDHA-Mediated NF-κB Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFangchen Gong,Ranran Li,Xiangtao Zheng,Weiwei Chen,Yanjun Zheng,Zhitao Yang,Ying Chen,Hongping Qu,Enqiang Mao,Erzhen ChenPubMed 34955649
- Clinical immunology (Orlando, Fla.) 205:65-742019Urine macrophages reflect kidney macrophage content during acute tubular interstitial and glomerular injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPing-Ping Sun et. al.PubMed 31212026
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 46.9 - 3000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 14.8 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
1 product for Alternative Version
Target data
Function
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed:12453413, PubMed:20581821, PubMed:27780864). Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis (By similarity). Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed:27780864). Can also bind siderophores from M.tuberculosis (PubMed:15642259, PubMed:21978368).
Alternative names
HNL, NGAL, LCN2, Neutrophil gelatinase-associated lipocalin, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Lipocalin-2, Oncogene 24p3, Siderocalin, p25
What's included?
Recommended products
Human Lipocalin-2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Lipocalin-2 with a sensitivity of 14.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 46.9 - 3000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 14.8 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Plasma EDTA | n 5 | C.V. 3.8 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Plasma EDTA | n 3 | C.V. 2.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 104 | Range 101 - 106 % |
Sample type Serum | Average % = 105 | Range 104 - 108 % |
Sample type EDTA Plasma | Average % = 109 | Range 107 - 111 % |
Sample type Saliva | Average % = 109 | Range 107 - 111 % |
Sample type Urine | Average % = 110 | Range 102 - 118 % |
Sample type Heparin Plasma | Average % = 103 | Range 99 - 107 % |
Sample type Citrate plasma | Average % = 106 | Range 104 - 109 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Lipocalin-2 ELISA Kit (ab215541) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Lipocalin-2 protein in edta plasma, hep plasma, saliva, serum, urine, cell culture supernatant, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Lipocalin-2 with 14.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Lipocalin-2 (also known as Neutrophil gelatinase-associated lipocalin or NGAL) is an iron binding and iron trafficking protein. Lipocalin-2 is involved in multiple cellular processes including apoptosis, innate immunity and renal development. Mice deficient in Lipocalin-2 appear normal but have increased susceptibility to bacterial infection. The bacteriostatic function may be related to Lipocalin-2 limiting bacterial iron supply. Human Lipocalin-2 has 62% protein sequence identity to mouse Lipocalin-2.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Sandwich ELISA - Human Lipocalin-2 ELISA Kit (ab215541)
Example of human Lipocalin-2 standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Lipocalin-2 ELISA Kit (ab215541)
Interpolated concentrations of native Lipocalin-2 in human serum and plasma samples.
The concentrations of Lipocalin-2 were measured in duplicates, interpolated from the Lipocalin-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2%, plasma (citrate) 2%, plasma (EDTA) 2%, and plasma (heparin) 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipocalin-2 concentration was determined to be 61.4 ng/mL in serum, 52.2 ng/mL in plasma (citrate), 85.5ng/mL in plasma (EDTA) and 52.4ng/mL in plasma (heparin).
Sandwich ELISA - Human Lipocalin-2 ELISA Kit (ab215541)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipocalin-2 concentration was determined to be 77 ng/mL with a range of 37 – 135 ng/mL.
Sandwich ELISA - Human Lipocalin-2 ELISA Kit (ab215541)
Interpolated concentrations of native Lipocalin-2 in human urine and saliva samples.
The concentrations of Lipocalin-2 were measured in duplicates, interpolated from the Lipocalin-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 3% and saliva 0.05%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipocalin-2 concentration was determined to be 67.9 ng/mL in urine and 1,551ng/mL in saliva.
Sandwich ELISA - Human Lipocalin-2 ELISA Kit (ab215541)
Interpolated concentrations of native Lipocalin-2 in human A431 cell culture supernatant and peripheral blood monocyte (PBMC) cell culture supernatant.
The concentrations of Lipocalin-2 were measured in duplicates, interpolated from the Lipocalin-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: A431 2% and PBMC 33%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipocalin-2 concentration was determined to be 65.8 ng/mL in A431 and 4.85 ng/mL in PBMC cell culture supernatant.
Sandwich ELISA - Human Lipocalin-2 ELISA Kit (ab215541)
Human Lipocalin-2 standard curve comparison data.
Standard curve comparison between human Lipocalin-2 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 2-fold increase in sensitivity.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com