AB215541

Human Lipocalin-2 ELISA Kit

Recombinant
SimpleStep
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(3 Publications)

Human Lipocalin-2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Lipocalin-2 with a sensitivity of 14.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

HNL, NGAL, LCN2, Neutrophil gelatinase-associated lipocalin, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Lipocalin-2, Oncogene 24p3, Siderocalin, p25

7 Images

ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

ELISA

Supplier Data

ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

sELISA

Supplier Data

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

Example of human Lipocalin-2 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

Interpolated concentrations of native Lipocalin-2 in human serum and plasma samples.

The concentrations of Lipocalin-2 were measured in duplicates, interpolated from the Lipocalin-2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2%, plasma (citrate) 2%, plasma (EDTA) 2%, and plasma (heparin) 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipocalin-2 concentration was determined to be 61.4 ng/mL in serum, 52.2 ng/mL in plasma (citrate), 85.5ng/mL in plasma (EDTA) and 52.4ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

Interpolated concentrations of native Lipocalin-2 in human urine and saliva samples.

The concentrations of Lipocalin-2 were measured in duplicates, interpolated from the Lipocalin-2 standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 3% and saliva 0.05%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipocalin-2 concentration was determined to be 67.9 ng/mL in urine and 1,551ng/mL in saliva.

sELISA

Unknown

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

Human Lipocalin-2 standard curve comparison data.

Standard curve comparison between human Lipocalin-2 SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 2-fold increase in sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipocalin-2 concentration was determined to be 77 ng/mL with a range of 37 – 135 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

Interpolated concentrations of native Lipocalin-2 in human A431 cell culture supernatant and peripheral blood monocyte (PBMC) cell culture supernatant.

The concentrations of Lipocalin-2 were measured in duplicates, interpolated from the Lipocalin-2 standard curves and corrected for sample dilution. Undiluted samples are as follows : A431 2% and PBMC 33%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipocalin-2 concentration was determined to be 65.8 ng/mL in A431 and 4.85 ng/mL in PBMC cell culture supernatant.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 14.8 pg/mL

Range

46.9 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Lipocalin-2 ELISA Kit ab215541 is a rapid single-wash 90-min Sandwich ELISA to measure Human Lipocalin-2 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, saliva, serum, urine. This SimpleStep sensitivity is 14.8 pg/mL.

How the assay works

Human Lipocalin-2 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Lipocalin-2 ELISA Kit ab215541 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Lipocalin-2 (also known as Neutrophil gelatinase-associated lipocalin or NGAL) is an iron binding and iron trafficking protein. Lipocalin-2 is involved in multiple cellular processes including apoptosis, innate immunity and renal development. Mice deficient in Lipocalin-2 appear normal but have increased susceptibility to bacterial infection. The bacteriostatic function may be related to Lipocalin-2 limiting bacterial iron supply. Human Lipocalin-2 has 62% protein sequence identity to mouse Lipocalin-2.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma EDTA", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" }, { "reproducibilityType": "Intra", "sample": "Plasma EDTA", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "101 - 106 %", "average": "= 104" }, { "sample": "Serum", "range": "104 - 108 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "107 - 111 %", "average": "= 109" }, { "sample": "Saliva", "range": "107 - 111 %", "average": "= 109" }, { "sample": "Urine", "range": "102 - 118 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "99 - 107 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "104 - 109 %", "average": "= 106" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab215541-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB215541-CMP04" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB215541-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB215541-CMP07" }, { "size":"1 x 6000 µL", "name":"10X Human Lipocalin-2 Capture Antibody", "number":"AB215541-CMP05" }, { "size":"2 x 10 Vial", "name":"Human Lipocalin-2 Lyophilized Recombinant Protein", "number":"AB215541-CMP08" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB215541-CMP01" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB215541-CMP02" }, { "size":"1 x 6000 µL", "name":"10X Human Lipocalin-2 Detector Antibody", "number":"AB215541-CMP06" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB215541-CMP09" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB215541-CMP03" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab215541-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB215541-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB215541-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB215541-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB215541-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB215541-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Lipocalin-2 Lyophilized Recombinant Protein", "number":"AB215541-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB215541-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Lipocalin-2 Capture Antibody", "number":"AB215541-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Lipocalin-2 Detector Antibody", "number":"AB215541-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB215541-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed : 12453413, PubMed : 20581821, PubMed : 27780864). Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation : iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis (By similarity). Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed : 27780864). Can also bind siderophores from M.tuberculosis (PubMed : 15642259, PubMed : 21978368).

See full target information LCN2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:4453 PubMed39910157

2025

The effect of prednisolone ingestion and acute exercise on lipocalin-2 and its variants in young men: a pilot randomised crossover study.

Applications

Unspecified application

Species

Unspecified reactive species

Carlie Bauer,Rhiannon K Patten,Qiyang Sun,Haoyun Li,Daniels Konja,Mary Woessner,Xuzhu Lin,Andrew Garnham,David L Hare,Peter R Ebeling,Marc Sim,Joshua R Lewis,Yu Wang,Lewan Parker,Itamar Levinger

Journal of inflammation research 14:7035-7051 PubMed34955649

2021

OLFM4 Regulates Lung Epithelial Cell Function in Sepsis-Associated ARDS/ALI via LDHA-Mediated NF-κB Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Fangchen Gong,Ranran Li,Xiangtao Zheng,Weiwei Chen,Yanjun Zheng,Zhitao Yang,Ying Chen,Hongping Qu,Enqiang Mao,Erzhen Chen

Clinical immunology (Orlando, Fla.) 205:65-74 PubMed31212026

2019

Urine macrophages reflect kidney macrophage content during acute tubular interstitial and glomerular injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ping-Ping Sun,Xu-Jie Zhou,Jian-Qun Su,Chen Wang,Xiao-Juan Yu,Tao Su,Gang Liu,Su-Xia Wang,Jing Nie,Li Yang

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Lipocalin-2 ELISA Kit

ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (3)

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