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AB212165

Human Lipoprotein A ELISA Kit

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(1 Review)

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(12 Publications )

Human Lipoprotein A ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human lipoprotein A with a sensitivity of 2.5 ng/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA with a 450 nm readout; compatible with any standard plate reader
- Broad sample compatibility – suitable for serum, plasma, urine, and cerebrospinal fluid
- Cited in 10 publications
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Sandwich ELISA - Human Lipoprotein A ELISA Kit (AB212165)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lipoprotein A ELISA Kit (AB212165)

Interpolated concentrations of native Lipoprotein A in human urine and cerebrospinal fluid samples.

The concentrations of Lipoprotein A were measured in duplicates, interpolated from the Lipoprotein A standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipoprotein A concentration was determined to be 500 ng/mL in urine, and 130 ng/mL in cerebrospinal fluid.

Sandwich ELISA - Human Lipoprotein A ELISA Kit (AB212165)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lipoprotein A ELISA Kit (AB212165)

Example of human Lipoprotein A standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Lipoprotein A ELISA Kit (AB212165)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lipoprotein A ELISA Kit (AB212165)

Serum from eight individual human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipoprotein A concentration was determined to be 169 μg/mL with a range of 42 – 576 μg/mL.

Sandwich ELISA - Human Lipoprotein A ELISA Kit (AB212165)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lipoprotein A ELISA Kit (AB212165)

Interpolated concentrations of native Lipoprotein A in human serum and plasma samples.

The concentrations of Lipoprotein A were measured in duplicate, interpolated from the Lipoprotein A standard curve, and corrected for sample dilution. Undiluted samples are as follows : serum 0.25%, plasma (citrate) 0.25%, plasma (heparin) 0.25% and plasma (EDTA) 0.125%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipoprotein A concentration was determined to be 309 μg/mL in serum, 291 μg/mL in plasma (citrate), 520 μg/mL in plasma (EDTA), and 150 μg/mL in plasma (Heparin).

Sandwich ELISA - Human Lipoprotein A ELISA Kit (AB212165)
  • sELISA

Lab

Sandwich ELISA - Human Lipoprotein A ELISA Kit (AB212165)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Urine, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.5 ng/mL

Range

17.2 - 1100 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Lipoprotein A ELISA Kit ab212165 is a rapid single-wash 90-min ELISA kit to measure Human Lipoprotein A in cerebral spinal fluid, urine, serum and plasma. This SimpleStep ELISA® sensitivity is 2.5 ng/mL.

How the assay works

Human Lipoprotein A SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native and recombinant human Lipoprotein A protein in serum, plasmas, urine, and cerebrospinal fluid samples only. Cellular and tissue extract samples have not been tested with this kit.

Human Lipoprotein A ELISA Kit ab212165 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human Lipoprotein A ELISA Kit ab212165

Human Lipoprotein A ELISA Kit ab212165 has been used to study the effects of LDL binding in obesity after bariatric surgery(1)and Lipoprotein A as a predictor of coronary events(2).

References:

(1)Olga Gurskyi et al. 2023, PMID: 37777014,
(2)Elena Aikawa et al. 2022, PMID: 35724702

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human Lipoprotein A Antibody Pair - BSA and Azide free ab241862
- Anti-Lipoprotein a antibody [EPR19731-148] - BSA and Azide free (Capture) ab242565
- Anti-Lipoprotein a antibody [EPR19731-165] - BSA and Azide free (Detector) ab242813

Related and recommended products

Human Lipoprotein A ELISA Kit ab212165 is commonly used to study cardiovascular diseases and lipid metabolism

Other ELISA kits to measure lipid metabolism are:

- Human Apoliporotein B ELISA kit ab190806

Lipoprotein A is an atherogenic lipoprotein particle formed by an assembly of LDL particles and apo(a) bound to apoB-100 component of LDL. Apolipoprotein A, the main constituent of Lipoprotein A, has serine proteinase activity and is capable of autoproteolysis. Apolipoprotein A has 4,548 amino acids, variable sizes from 200 to 700 kDa, multiple isoforms, and structural homology with plasminogen. It competes with plasminogen for its binding site, inhibiting tissue-type plasminogen activator 1 and leading to reduced fibrinolysis. High levels of Lipoprotein A in the blood is a risk factor for myocardial infarction (MI), coronary heart disease (CHD), cerebrovascular disease (CVD), atherosclerosis, thrombosis, and stroke.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.8" } ]
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Recovery

[ { "sample": "Serum", "range": "97 - 103 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "101 - 108 %", "average": "= 104" }, { "sample": "Cerebral Spinal Fluid", "range": "108 - 110 %", "average": "= 109" }, { "sample": "Urine", "range": "107 - 108 %", "average": "= 107" }, { "sample": "Heparin Plasma", "range": "100 - 107 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "99 - 105 %", "average": "= 102" }, { "sample": "Cell culture supernatant", "range": "99 - 105 %", "average": "= 102" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lipoprotein a also known as Lp(a) or LPA is a lipoprotein particle in the bloodstream characterized by the binding of apolipoprotein(a) to apolipoprotein B-100 on a low-density lipoprotein (LDL) core. It has a molecular weight that varies depending on the size of apolipoprotein(a) typically ranging from 300 to 700 kDa. This protein is primarily synthesized in the liver and its levels are mostly determined by genetic factors. Lp(a) has structural similarity to LDL and plasminogen each playing distinct roles in the body.
Biological function summary

Lp(a) is involved in lipid metabolism and occasionally plays a role in the modulation of fibrinolysis due to its structural resemblance to plasminogen. This resemblance allows it to competitively inhibit plasminogen activation which may influence clot stabilization and breakdown. Lp(a) does not appear to form part of a larger functional complex but can affect processes where plasminogen is active. It plays a dual role in both lipid transport and coagulation processes.

Pathways

The role of Lp(a) is significant in the cardiovascular system particularly in the atherosclerosis pathway. It interacts mainly through its structural mimicry of LDL and plasminogen leading to competitive interactions with these molecules. Lp(a)'s impact on the pathway involves promoting the formation of atherosclerotic plaques by depositing within arterial walls and contributing to pro-inflammatory activities. The primary proteins related in this context include LDL components and plasminogen.

Lp(a) levels above 200 nanomoles per liter are associated with an increased risk of cardiovascular diseases especially atherosclerosis and myocardial infarction. These elevated levels can lead to enhanced plaque formation in the arteries due to Lp(a)'s affinity for binding to phospholipids and arterial wall components. Furthermore it shares pathway associations with apolipoprotein B-100 linking it to familial hypercholesterolemia where abnormalities in lipid metabolism are evident. Both genetic and environmental factors contribute to the variation in Lp(a) levels and its pathological implications in these conditions.
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Product protocols

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Target data

Apo(a) is the main constituent of lipoprotein(a) (Lp(a)). It has serine proteinase activity and is able of autoproteolysis. Inhibits tissue-type plasminogen activator 1. Lp(a) may be a ligand for megalin/Gp 330.
See full target information LPA

Alternative Names

Apolipoprotein(a), Apo(a), Lp(a), LPA
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Reviews in cardiovascular medicine 24:322 PubMed39076454

2024

Elevated Lipoprotein(a) Levels are Associated with Arterial Stiffness Measured by Cardio-Ankle Vascular Index in Patients Undergoing Peritoneal Dialysis.

Applications

Unspecified application

Species

Unspecified reactive species

Po-Yu Huang,Bang-Gee Hsu,Huei-Jhen Lin,Yu-Li Lin,Chih-Hsien Wang,Jen-Pi Tsai

Medical science monitor : international medical journal of experimental and clinical research 30:e944348 PubMed38835156

2024

Serum Lipoprotein(a) Levels as a Predictor of Aortic Stiffness in Patients on Long-Term Peritoneal Dialysis.

Applications

Unspecified application

Species

Unspecified reactive species

Po-Yu Huang,Bang-Gee Hsu,Yu-Li Lin,Chi-Chong Tang,Hung-Hsiang Liou,Jen-Pi Tsai

Journal of lipid research 64:100451 PubMed37777014

2023

LDL binding to cell receptors and extracellular matrix is proatherogenic in obesity but improves after bariatric surgery.

Applications

Unspecified application

Species

Unspecified reactive species

Shobini Jayaraman,Antonio Pérez,Inka Miñambres,Jose Luis Sánchez-Quesada,Olga Gursky

Research and practice in thrombosis and haemostasis 7:102164 PubMed37680312

2023

Plasma tissue-type plasminogen activator is associated with lipoprotein(a) and clinical outcomes in hospitalized patients with COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyu Zhang,Wen Dai,Wen Zhu,Maya Rodriguez,Hayley Lund,Yuhe Xia,Yiliang Chen,Mary Rau,Ellen Anje Schneider,Mary Beth Graham,Shawn Jobe,Demin Wang,Weiguo Cui,Renren Wen,Sidney W Whiteheart,Jeremy P Wood,Roy Silverstein,Jeffery S Berger,Lisa Baumann Kreuziger,Tessa J Barrett,Ze Zheng

Communications biology 6:452 PubMed37095219

2023

A human iPSC-derived hepatocyte screen identifies compounds that inhibit production of Apolipoprotein B.

Applications

Unspecified application

Species

Unspecified reactive species

Jui-Tung Liu,Caren Doueiry,Yu-Lin Jiang,Josef Blaszkiewicz,Mary Paige Lamprecht,James A Heslop,Yuri K Peterson,Juliana Debrito Carten,Paula Traktman,Yang Yuan,Salman R Khetani,Waleed O Twal,Stephen A Duncan

PloS one 17:e0275901 PubMed36260611

2022

A liver secretome gene signature-based approach for determining circulating biomarkers of NAFLD severity.

Applications

Unspecified application

Species

Unspecified reactive species

Christoffer A Hagemann,Christian Legart,Mathias B Møllerhøj,Martin R Madsen,Henrik H Hansen,Merete J Kønig,Frederik Helgstrand,Flemming P Hjørne,Anders Toxværd,Jill L Langhoff,Urd L Kielgast,Lise L Gluud,Helene Ægidius,Kristoffer T G Rigbolt,Tina Vilsbøll,Jacob Jelsing,Filip K Knop

Journal of lipid research 63:100242 PubMed35724702

2022

Elevated lipoprotein(a) as a predictor for coronary events in older men.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Bartoli-Leonard,Mandy E Turner,Jonas Zimmer,Roland Chapurlat,Tan Pham,Masanori Aikawa,Aruna D Pradhan,Pawel Szulc,Elena Aikawa

Frontiers in cardiovascular medicine 9:778919 PubMed35155626

2022

Lipoprotein(a) Induces Vesicular Cardiovascular Calcification Revealed With Single-Extracellular Vesicle Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Maximillian A Rogers,Samantha K Atkins,Kang H Zheng,Sasha A Singh,Sarvesh Chelvanambi,Tan H Pham,Shiori Kuraoka,Erik S G Stroes,Masanori Aikawa,Elena Aikawa

Oxidative medicine and cellular longevity 2022:9964689 PubMed35096275

2022

Apolipoprotein (a)/Lipoprotein(a)-Induced Oxidative-Inflammatory 7-nAChR/p38 MAPK/IL-6/RhoA-GTP Signaling Axis and M1 Macrophage Polarization Modulate Inflammation-Associated Development of Coronary Artery Spasm.

Applications

Unspecified application

Species

Unspecified reactive species

Yen-Kuang Lin,Chi-Tai Yeh,Kuang-Tai Kuo,Iat-Hang Fong,Vijesh Kumar Yadav,Nicholas G Kounis,Patrick Hu,Ming-Yow Hung

Annals of the New York Academy of Sciences 1510:167-179 PubMed34951699

2021

Identification of maternal serum biomarkers for prenatal diagnosis of nonsyndromic orofacial clefts.

Applications

Unspecified application

Species

Unspecified reactive species

Xinhuan Wang,Xiaohong Yang,Pei Huang,Xiujiao Meng,Zhuan Bian,Liuyan Meng
View all publications
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