AB309391
Human Liver Carboxylesterase 1/CES1 ELISA Kit
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Human Liver Carboxylesterase 1/CES1 ELISA Kit is a Sandwich ELISA for the measurement of Human Liver Carboxylesterase 1/CES1 in Human in Cell Culture Media, Biofluids samples.
View Alternative Names
CES2, SES1, CES1, Liver carboxylesterase 1, Acyl-coenzyme A:cholesterol acyltransferase, Brain carboxylesterase hBr1, Carboxylesterase 1, Cholesteryl ester hydrolase, Cocaine carboxylesterase, Egasyn, HMSE, Methylumbelliferyl-acetate deacetylase 1, Monocyte/macrophage serine esterase, Retinyl ester hydrolase, Serine esterase 1, Triacylglycerol hydrolase, ACAT, CE-1, hCE-1, CEH, REH, TGH
1 Images
- sELISA
Supplier Data
Sandwich ELISA - Human Liver Carboxylesterase 1/CES1 ELISA Kit (AB309391)
Example of standard curve using ab309391. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
Complementary Products
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
Product details
Human Liver Carboxylesterase 1/CES1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human liver Carboxylesterase 1/CES1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human liver Carboxylesterase 1/CES1 coated on a 96-well plate.
Recovery
[ { "sample": "Plasma", "range": "78 - 99 %", "average": "= 86.95" }, { "sample": "Serum", "range": "81 - 100 %", "average": "= 89.54" }, { "sample": "Cell culture media", "range": "77 - 103 %", "average": "= 90.33" } ]
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
Product protocols
- Visit the General protocols
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Target data
Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs (PubMed : 18762277, PubMed : 7980644, PubMed : 9169443, PubMed : 9490062). Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester (PubMed : 18762277, PubMed : 7980644, PubMed : 9169443, PubMed : 9490062). Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine (PubMed : 7980644). Catalyzes the transesterification of cocaine to form cocaethylene (PubMed : 7980644). Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate (PubMed : 7980644). Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes of 2-arachidonoylglycerol and prostaglandins (PubMed : 21049984). Hydrolyzes cellular cholesteryl esters to free cholesterols and promotes reverse cholesterol transport (RCT) by facilitating both the initial and final steps in the process (PubMed : 11015575, PubMed : 16024911, PubMed : 16971496, PubMed : 18762277). First of all, allows free cholesterol efflux from macrophages to extracellular cholesterol acceptors and secondly, releases free cholesterol from lipoprotein-delivered cholesteryl esters in the liver for bile acid synthesis or direct secretion into the bile (PubMed : 16971496, PubMed : 18599737, PubMed : 18762277).
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