Human Liver-FABP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Liver-FABP with a sensitivity of 9.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Free radical biology & medicine 207:260-2712023Derlin-1 ameliorates nonalcoholic hepatic steatosis by promoting ubiquitylation and degradation of FABP1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui You,Xin Wen,Xingchun Wang,Cuiling Zhu,Haibing Chen,Le Bu,Jun Zhang,Shen QuPubMed 37499886
- Frontiers in endocrinology 13:9931372022Association between liver-type fatty acid-binding protein and hyperuricemia before and after laparoscopic sleeve gastrectomy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui You,Huihui Ma,Xingchun Wang,Xin Wen,Cuiling Zhu,Wangjia Mao,Le Bu,Manna Zhang,Jiajing Yin,Lei Du,Xiaoyun Cheng,Haibing Chen,Jun Zhang,Shen QuPubMed 36277716
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 14.8 - 950 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 9.4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PubMed:25732850). Binds cholesterol (PubMed:25732850). Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport (By similarity).
Alternative names
FABPL, FABP1, Fatty acid-binding protein 1, Liver-type fatty acid-binding protein, L-FABP
What's included?
Recommended products
Human Liver-FABP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Liver-FABP with a sensitivity of 9.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 14.8 - 950 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 9.4 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 5 | mean - | SD - | C.V. 6.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 4.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 103 | Range 97 - 113 % |
Sample type Serum | Average % = 97 | Range 93 - 101 % |
Sample type EDTA Plasma | Average % = 93 | Range 87 - 98 % |
Sample type Urine | Average % = 104 | Range 101 - 105 % |
Sample type Heparin Plasma | Average % = 85 | Range 81 - 92 % |
Sample type Citrate plasma | Average % = 88 | Range 82 - 94 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Liver-FABP ELISA Kit (ab218261) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Liver-FABP protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Liver-FABP with 9.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Liver-FABP is a 14 kDa member of the FABP family. Liver-FABP binds and transports cholesterol, free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules. A variety of cells express Liver-FABP, including hepatocytes, simple columnar epithelium of the small intestine, and proximal tubular epithelium of the kidney.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Liver fatty acid binding protein (liver FABP) also known as L-FABP or FABP1 is a small protein with a mass of approximately 14 kilodaltons. It functions mainly in the liver where it binds free fatty acids and other lipophilic substances facilitating their transport within cells. This protein is highly expressed in hepatocytes and also found in the small intestine and kidneys. Its role in binding fatty acids positions it as an important mediator in lipid metabolism making it of interest in a variety of metabolic studies.
- Biological function summary
Liver FABP is essential for maintaining lipid homeostasis within cells. It is not part of a larger complex but acts by itself to regulate the intracellular concentration of lipids and protect cells from lipotoxicity. By sequestering fatty acids it prevents these molecules from disrupting cellular membranes or signaling pathways. Liver FABP also participates in the uptake transport and metabolic conversion of fatty acids and their metabolites influencing energy homeostasis and other vital processes.
- Pathways
Liver FABP plays a significant role in the fatty acid metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathways. It acts by modulating the availability of lipid ligands necessary for PPAR activation linking it functionally to these nuclear receptors that control gene expression involved in lipid and glucose metabolism. Liver FABP's association with proteins like FABP2 and FABP4 within these pathways provides insight into its broader metabolic network highlighting its interactions in fatty acid transport and metabolism.
- Associated diseases and disorders
Liver FABP shows a strong connection to metabolic conditions particularly non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes. Its dysregulation is often observed in these disorders which include altered lipid profiles and insulin resistance. Liver FABP is also linked to certain forms of cancer with aberrant expression levels found in some tumor types. The interactions of liver FABP with proteins such as FABP5 in these diseases suggest a potential role in the pathogenesis of metabolic disorders and tumor development making it a candidate for further research and therapeutic targeting.
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3 product images
Sandwich ELISA - Human Liver-FABP ELISA Kit (ab218261)
Example of Human Liver-FABP standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Liver-FABP ELISA Kit (ab218261)
Interpolated concentrations of native Liver-FABP in human serum, plasma, urine, and cell culture supernatant samples.
The concentrations of Liver-FABP were measured in duplicates, interpolated from the Liver-FABP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 7.5%, plasma (citrate) 7.5%, plasma (heparin) 7.5%, plasma EDTA) 7.5%, urine 50 %, and spleen HepG2 supernatant 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Liver-FABP concentration was determined to be 11.2 ng/mL in serum, 12.2 ng/mL in plasma (citrate) and 13.6 ng/mL in plasma (heparin), 11.0 ng/mL in plasma (EDTA), 0.682 ng/mL in urine, and 11.4 ng/mL in HepG2 supernatant.
Sandwich ELISA - Human Liver-FABP ELISA Kit (ab218261)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Liver-FABP concentration was determined to be 10.3 ng/mL with a range of 6.36 – 19.0 ng/mL.
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