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AB313985

Human Livin ELISA Kit

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Human Livin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Livin in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

KIAP, LIVIN, MLIAP, RNF50, UNQ5800/PRO19607/PRO21344, BIRC7, Baculoviral IAP repeat-containing protein 7, Kidney inhibitor of apoptosis protein, Livin, Melanoma inhibitor of apoptosis protein, RING finger protein 50, RING-type E3 ubiquitin transferase BIRC7, ML-IAP

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Sandwich ELISA - Human Livin ELISA Kit (AB313985)
  • sELISA

Supplier Data

Sandwich ELISA - Human Livin ELISA Kit (AB313985)

Example of standard curve using ab313985. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.1 ng/mL

Range

0.1 - 25 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Livin ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Livin in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Livin coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Bad, Bax, Bcl-2, bcl-w, BID, BIM, Caspase-3, Caspase-8, CD40, CD40 Ligand, cIAP-2, Cytochrome C, DR6, Fas, Fas Ligand, HSP-27, HSP-60, HSP-70, HTRA2, IGF-1, IGF-2, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGF1 R, p21, p27, p53, SMAC, Survivin, TNF RI, TNF RII, TNF-alpha, TNF-beta, TRAIL R1, TRAIL R2, TRAIL R3, TRAIL R4, XIAP.

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Recovery

[ { "sample": "Serum", "range": "70 - 84 %", "average": "= 74.6" }, { "sample": "Plasma", "range": "68 - 79 %", "average": "= 71.29" }, { "sample": "Cell culture media", "range": "75 - 94 %", "average": "= 83.09" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313985-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB313985-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Livin Detection Antibody", "number":"AB313985-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Livin Protein Standard", "number":"AB313985-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Livin Antibody-coated ELISA Plate", "number":"AB313985-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313985-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB313985-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313985-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313985-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313985-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis, cell proliferation, and cell cycle control (PubMed : 11024045, PubMed : 11084335, PubMed : 11162435, PubMed : 16729033, PubMed : 17294084). Its anti-apoptotic activity is mediated through the inhibition of CASP3, CASP7 and CASP9, as well as by its E3 ubiquitin-protein ligase activity (PubMed : 11024045, PubMed : 16729033). As it is a weak caspase inhibitor, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival (PubMed : 16729033). May contribute to caspase inhibition, by blocking the ability of DIABLO/SMAC to disrupt XIAP/BIRC4-caspase interactions (PubMed : 16729033). Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine (PubMed : 11084335, PubMed : 11162435, PubMed : 11865055). Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2 (PubMed : 11865055). This activation depends on TAB1 and MAP3K7/TAK1 (PubMed : 11865055). In vitro, inhibits CASP3 and proteolytic activation of pro-CASP9 (PubMed : 11024045).. Isoform 1. Blocks staurosporine-induced apoptosis (PubMed : 11322947). Promotes natural killer (NK) cell-mediated killing (PubMed : 18034418).. Isoform 2. Blocks etoposide-induced apoptosis (PubMed : 11162435, PubMed : 11322947). Protects against natural killer (NK) cell-mediated killing (PubMed : 18034418).
See full target information BIRC7
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Complementary Products

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AB97350

Anti-Livin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Livin antibody (AB97350)

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Proteins & Peptides

AB87179

Recombinant Human Livin protein

SDS-PAGE - Recombinant Human Livin protein (AB87179)

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Product promise

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