JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB233612

Human LKB1 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human LKB1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human LKB1 with a sensitivity of 1 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

LKB1, PJS, STK11, Serine/threonine-protein kinase STK11, Liver kinase B1, Renal carcinoma antigen NY-REN-19, hLKB1

3 Images
Sandwich ELISA - Human LKB1 ELISA Kit (AB233612)
  • sELISA

Supplier Data

Sandwich ELISA - Human LKB1 ELISA Kit (AB233612)

Interpolated concentrations of native LKB1 in mouse RAW264.7 and rat PC-12 cell extract, extract based on a 750 μg/mL extract load.

The concentrations of LKB1 were measured in duplicate and interpolated from the LKB1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LKB1 concentration was determined to be 61 ng/mL in mouse RAW 264.7 cell extract and 11 ng/mL in rat PC12.

Sandwich ELISA - Human LKB1 ELISA Kit (AB233612)
  • sELISA

Supplier Data

Sandwich ELISA - Human LKB1 ELISA Kit (AB233612)

Interpolated concentrations of native LKB1 in human MCF-7 cell extract based on a 3,000 μg/mL extract load and HEK293T cell extract based on a 1,500 μg/mL extract load.

The concentrations of LKB1 were measured in duplicate and interpolated from the LKB1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LKB1 concentration was determined to be 27 ng/mL in MCF-7 cell extract and 46.3 ng/mL in HEK293T cell extract.

Sandwich ELISA - Human LKB1 ELISA Kit (AB233612)
  • sELISA

Supplier Data

Sandwich ELISA - Human LKB1 ELISA Kit (AB233612)

Example of human LKB1 standard curve in 1X Cell Extraction Buffer PTR.

The LKB1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Human, Mouse, Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 1 ng/mL

Range

3.13 - 200 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human LKB1 ELISA Kit (ab233612) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LKB1 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human LKB1 with 1 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "HEK-293T", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" }, { "reproducibilityType": "Intra", "sample": "HEK-293T", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]
style
left-column

Recovery

[ { "sample": "Cell culture extracts", "range": "94 - 108 %", "average": "= 100" }, { "sample": "Tissue", "range": "100 - 118 %", "average": "= 108" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab233612-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB233612-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB233612-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB233612-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB233612-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB233612-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LKB1 Capture Antibody", "number":"AB233612-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human LKB1 Lyophilized Recombinant Protein", "number":"AB233612-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB233612-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LKB1 Detector Antibody", "number":"AB233612-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB233612-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB233612-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LKB1 also known as STK11 is a serine/threonine kinase with a molecular weight of approximately 48 kDa. This protein is widely expressed in various tissues including the liver pancreas and skeletal muscle. LKB1 plays an essential role in cellular energy homeostasis by phosphorylating and activating a group of at least 14 different proteins within the AMP-activated protein kinase (AMPK) family. The LKB1 protein acts as a master upstream kinase ensuring proper cellular energy balance and metabolism under conditions of nutrient stress.
Biological function summary

LKB1 is involved in controlling cell growth polarity and metabolism. It forms a complex with STRAD and MO25 which are necessary for its activation and function. By influencing these biological processes LKB1 regulates cell cycle arrest in response to stress signals and contributes to maintaining cellular integrity. The regulation of these processes positions LKB1 as an important factor in managing how cells respond to metabolic challenges.

Pathways

Its function integrates into major signaling networks like the AMPK and mTOR pathways. LKB1 activates AMPK which in turn affects mTOR signaling a pathway critical for cell growth and autophagy regulation. LKB1's interaction with AMPK links cellular energy levels to the control of biosynthetic pathways and cell cycle progression. The LKB1-AMPK-mTOR axis for instance highlights the protein's role in managing how cells balance growth-related activities and energy conservation.

LKB1 mutations or malfunctions are linked to Peutz-Jeghers syndrome and various forms of cancer such as lung cancer. Altered LKB1 activity affects its ability to suppress tumors primarily through its pathway interactions with AMPK and mTOR. Dysfunction in this pathway leads to unregulated cell growth and subsequently tumorigenesis. Understanding LKB1's role in these conditions aids in developing targeted therapies and diagnostic tools to manage associated risks and interventions effectively.
style
left-column

Product protocols

style
left-column

Target data

Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity : phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including : inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response : interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1. Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53 : translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with NUAK1, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair (PubMed : 25329316).. Isoform 2. Has a role in spermiogenesis.
See full target information STK11
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Primary Antibodies

AB199970

Anti-LKB1 antibody [EPR19379]

KO Validated|RabMAb|Recombinant

Western blot - Anti-LKB1 antibody [EPR19379] (AB199970)

  • 5
  • 1
Assay Kits

AB224878

Mouse p53 ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse p53 ELISA Kit (AB224878)

  • 0
  • 0
Primary Antibodies

AB188865

Anti-TXNIP antibody [EPR14774]

RabMAb|Recombinant|KO Validated

Western blot - Anti-TXNIP antibody [EPR14774] (AB188865)

  • 4
  • 4
Primary Antibodies

AB179515

Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883]

RabMAb|Recombinant

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515)

  • 3
  • 3

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com