AB212161

Human LOX1 ELISA Kit

Recombinant
SimpleStep
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(7 Publications)

Human LOX1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human LOX1 with a sensitivity of 6.85 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

CLEC8A, LOX1, OLR1, Oxidized low-density lipoprotein receptor 1, Ox-LDL receptor 1, C-type lectin domain family 8 member A, Lectin-like oxidized LDL receptor 1, Lectin-type oxidized LDL receptor 1, LOX-1, Lectin-like oxLDL receptor 1, hLOX-1

6 Images

Sandwich ELISA - Human LOX1 ELISA Kit (AB212161)

sELISA

Supplier Data

Sandwich ELISA - Human LOX1 ELISA Kit (AB212161)

Interpolated concentrations of native LOX1 in human Hela and placenta extract based on an 80 μg/mL and 500 μg/mL extract load.

The concentrations of LOX1 were measured in duplicate and interpolated from the LOX1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LOX1 concentration was determined to be 927 pg/mL in Hela extract and 390 pg/mL in placenta extract.

sELISA

Supplier Data

Sandwich ELISA - Human LOX1 ELISA Kit (AB212161)

Serum from three individual high LDL human donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LOX1 concentration was determined to be 198 pg/mL with a range of 0 – 413 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human LOX1 ELISA Kit (AB212161)

Example of human LOX1 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human LOX1 ELISA Kit (AB212161)

Interpolated concentrations of native LOX1 in human serum and plasma samples.

The concentrations of LOX1 were measured in duplicates, interpolated from the LOX1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (heparin) 100% and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LOX1 concentration was determined to be 322 pg/mL in serum, 101 pg/mL in plasma (citrate), 106 pg/mL in plasma (heparin) and 165 pg/mL in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Human LOX1 ELISA Kit (AB212161)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LOX1 concentration was determined to be 211 pg/mL with a range of 83.5 – 745 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human LOX1 ELISA Kit (AB212161)

Example of human LOX1 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 6.85 pg/mL

Range

15.625 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human LOX1 ELISA Kit (ab212161) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LOX1 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human LOX1 with NULL NULL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

LOX1 is a receptor expressed on several cell types which mediate a variety of cellular processes. On vascular endothelial cell, LOX1 is involved in the internalization and degradation of oxidatively modified low density lipoprotein (oxLDL). In addition to this role, it is a receptor for HSP70 protein and is a part of antigen cross presentation in dendritic cells to native T-cells. It is also a receptor f advanced glycation end products, activated platelets, monocytes, apoptotic cells and Gram-negative and Gram-positive bacteria.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.6" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "112 - 120 %", "average": "= 116" }, { "sample": "Serum", "range": "98 - 114 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "108 - 111 %", "average": "= 109" }, { "sample": "Cell culture extracts", "range": "97 - 99 %", "average": "= 98" }, { "sample": "Tissue Extracts", "range": "99 - 106 %", "average": "= 103" }, { "sample": "Heparin Plasma", "range": "104 - 106 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "99 - 113 %", "average": "= 105" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab212161-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB212161-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB212161-CMP13", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human LOX1 Lyophilized Recombinant Protein", "number":"AB212161-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB212161-CMP12", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB212161-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LOX1 Capture Antibody", "number":"AB212161-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB212161-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LOX1 Detector Antibody", "number":"AB212161-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB212161-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB212161-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB212161-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB212161-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.. (Microbial infection) May serve as a receptor for adhesin A variant 3 (nadA) of N.meningitidis.

See full target information OLR1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

BMC nephrology 25:427 PubMed39604892

2024

The relationship between serum levels of LOX-1, hs-cTnT, NGAL, and renal function, and their diagnostic value in patients with chronic kidney disease: a retrospective study.

Applications

Unspecified application

Species

Unspecified reactive species

Liyin Chai,Jun Zeng,Li Gong,Zhuhong Li,Fang Wang,Zhengyang Liu,Wang Fan,Bingbing Shen

Frontiers in neuroscience 18:1442110 PubMed39234183

2024

Lectin-type oxidized LDL receptor-1 as a potential therapeutic target for cerebral cavernous malformations treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Karthik Ashok,Tyra Martinez,Julie Sesen,Sana Nasim,Shih-Shan Lang,Gregory Heuer,Alexander Tucker,Miguel Alejandro Lopez-Ramirez,Edward R Smith,Aram Ghalali

Biomolecules 13: PubMed37627252

2023

Serum Soluble Lectin-like Oxidized Low-Density Lipoprotein Receptor-1 (sLOX-1) Is Associated with Atherosclerosis Severity in Coronary Artery Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Katharine A Kott,Elijah Genetzakis,Michael P Gray,Peter Hansen,Helen M McGuire,Jean Y Yang,Stuart M Grieve,Stephen T Vernon,Gemma A Figtree

Canadian respiratory journal 2022:6619331 PubMed35449557

2022

Expression of Serum sLOX-1 in Patients with Non-Small-Cell Lung Cancer and Its Correlation with Lipid Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Fangfang Hao,Jinliang Chen,Jinnan Wu,Xin Ge,Xuedong Lv,Dongmei Zhang,Jianrong Chen

Journal of infection in developing countries 15:289-296 PubMed33690213

2021

Cardiovascular risks in Asian HIV-infected patients receiving boosted-protease inhibitor-based antiretroviral treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Sakaewan Ounjaijean,Kanokwan Kulprachakarn,Linda Aurpibul,Quanhathai Kaewpoowat,Kongsak Boonyapranai,Romanee Chaiwarith,Supapong Arwon,Khuanchai Supparatpinyo,Kittipan Rerkasem

Yonsei medical journal 61:720-725 PubMed32734736

2020

Soluble Lectin-Like Oxidized Low-Density Lipoprotein Receptor 1 Is Inversely Correlated with the Activity of ANCA-Associated Vasculitis.

Applications

Unspecified application

Species

Unspecified reactive species

Taejun Yoon,Sung Soo Ahn,Jason Jungsik Song,Yong Beom Park,Sang Won Lee

Diagnostics (Basel, Switzerland) 10: PubMed32340234

2020

Increased Tissue Expression of Lectin-Like Oxidized LDL Receptor-1 (LOX-1) Is Associated with Disease Severity in Chronic Rhinosinusitis with Nasal Polyps.

Applications

Unspecified application

Species

Unspecified reactive species

Manabu Nishida,Sachio Takeno,Kohta Takemoto,Daisuke Takahara,Takao Hamamoto,Takashi Ishino,Tomohiro Kawasumi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human LOX1 ELISA Kit

Sandwich ELISA - Human LOX1 ELISA Kit (AB212161)

Sandwich ELISA - Human LOX1 ELISA Kit (AB212161)

Sandwich ELISA - Human LOX1 ELISA Kit (AB212161)

Sandwich ELISA - Human LOX1 ELISA Kit (AB212161)

Sandwich ELISA - Human LOX1 ELISA Kit (AB212161)

Sandwich ELISA - Human LOX1 ELISA Kit (AB212161)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (7)

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Secondary Antibodies

Primary Antibodies

Product promise