AB235643

Human Lp-PLA2 ELISA Kit

Recombinant
SimpleStep
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(4 Publications)

Human Lp-PLA2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Lp-PLA2 with a sensitivity of 45 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

PAFAH, PLA2G7, Platelet-activating factor acetylhydrolase, PAF acetylhydrolase, 1-alkyl-2-acetylglycerophosphocholine esterase, 2-acetyl-1-alkylglycerophosphocholine esterase, Group-VIIA phospholipase A2, LDL-associated phospholipase A2, PAF 2-acylhydrolase, gVIIA-PLA2, LDL-PLA(2)

5 Images

Sandwich ELISA - Human Lp-PLA2 ELISA Kit (AB235643)

sELISA

Supplier Data

Sandwich ELISA - Human Lp-PLA2 ELISA Kit (AB235643)

Interpolated concentrations of native Lp-PLA2 in human serum, plasma and cell culture supernatant samples.

The concentrations of Lp-PLA2 were measured in duplicates, interpolated from the Lp-PLA2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 50, plasma (citrate) 1 : 50, plasma (EDTA) 1 : 50, and plasma (heparin) 1 : 50. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lp-PLA2 concentration was determined to be 175 ng/mL in serum, 102 ng/mL in plasma (citrate), 168 ng/mL in plasma (EDTA) and 118 ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human Lp-PLA2 ELISA Kit (AB235643)

Example of human Lp-PLA2 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Lp-PLA2 ELISA Kit (AB235643)

Interpolated concentrations of native Lp-PLA2 in human cell culture supernatant samples.

The concentrations of Lp-PLA2 were measured in duplicates, interpolated from the Lp-PLA2 standard curves and corrected for sample dilution. Undiluted samples are as follows : Unstimulated 10%, Stimulated 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lp-PLA2 concentration was determined to be 2.1 ng/mL in unstimulated PBMC cell culture supernatant and 9.3 ng/mL in PHA-M Stimulated cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human Lp-PLA2 ELISA Kit (AB235643)

Human Lp-PLA2 Standard Curve Comparison.

Standard Curve comparison between Human Lp-PLA2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human Lp-PLA2 ELISA Kit (AB235643)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lp-PLA2 concentration was determined to be 275 ng/mL with a range of 129 – 436 ng/mL.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 45 pg/mL

Range

0.16 - 10 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Lp-PLA2 ELISA Kit (ab235643) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Lp-PLA2 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Lp-PLA2 with 45 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]

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Recovery

[ { "sample": "Serum", "range": "93 - 97 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "72 - 88 %", "average": "= 81" }, { "sample": "Heparin Plasma", "range": "93 - 97 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "97 - 100 %", "average": "= 98" }, { "sample": "Cell culture media", "range": "99 - 102 %", "average": "= 101" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab235643-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB235643-CMP03" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB235643-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB235643-CMP04" }, { "size":"2 x 10 Vial", "name":"Human Lp-PLA2 Lyophilized Recombinant Protein", "number":"AB235643-CMP08" }, { "size":"1 x 6000 µL", "name":"10X Human Lp-PLA2 Capture Antibody", "number":"AB235643-CMP06" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB235643-CMP05" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB235643-CMP01" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB235643-CMP09" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB235643-CMP02" }, { "size":"1 x 6000 µL", "name":"10X Human Lp-PLA2 Detector Antibody", "number":"AB235643-CMP07" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab235643-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB235643-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB235643-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB235643-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB235643-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB235643-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Lp-PLA2 Lyophilized Recombinant Protein", "number":"AB235643-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Lp-PLA2 Capture Antibody", "number":"AB235643-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB235643-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Lp-PLA2 Detector Antibody", "number":"AB235643-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB235643-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress response (PubMed : 10066756, PubMed : 16371369, PubMed : 17090529, PubMed : 2040620, PubMed : 7700381, PubMed : 8624782). At the lipid-aqueous interface, hydrolyzes the ester bond of fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) (PubMed : 10504265, PubMed : 2040620). Specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position (PubMed : 2040620). Can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups (PubMed : 2040620, PubMed : 8624782). Hydrolyzes and inactivates platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent pro-inflammatory signaling lipid that acts through PTAFR on various innate immune cells (PubMed : 10066756, PubMed : 10504265, PubMed : 11590221, PubMed : 16371369, PubMed : 18434304, PubMed : 7592717, PubMed : 7700381, PubMed : 8624782, PubMed : 8675689). Hydrolyzes oxidatively truncated phospholipids carrying an aldehyde group at omega position, preventing their accumulation in low-density lipoprotein (LDL) particles and uncontrolled pro-inflammatory effects (PubMed : 2040620, PubMed : 7700381). As part of high-density lipoprotein (HDL) particles, can hydrolyze phospholipids having long-chain fatty acyl hydroperoxides at sn-2 position and protect against potential accumulation of these oxylipins in the vascular wall (PubMed : 17090529). Catalyzes the release from membrane phospholipids of F2-isoprostanes, lipid biomarkers of cellular oxidative damage (PubMed : 16371369).

See full target information PLA2G7

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 14:1405431 PubMed39507948

2024

Non-classical monocyte levels correlate negatively with HIV-associated cerebral small vessel disease and cognitive performance.

Applications

Unspecified application

Species

Unspecified reactive species

Meera V Singh,Md Nasir Uddin,Mae Covacevich Vidalle,Karli R Sutton,Zachary D Boodoo,Angelique N Peterson,Alicia Tyrell,Madalina E Tivarus,Henry Z Wang,Bogachan Sahin,Jianhui Zhong,Miriam T Weber,Lu Wang,Xing Qiu,Sanjay B Maggirwar,Giovanni Schifitto

World journal of psychiatry 14:380-387 PubMed38617987

2024

Evaluating serum CXCL12, sCD22, Lp-PLA2 levels and ratios as biomarkers for diagnosis of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Zeng-Ling Liu,Fei-Fei Hua,Lei Qu,Na Yan,Hui-Fang Zhang

Molecular cell 82:1992-2005.e9 PubMed35417664

2022

Lyso-PAF, a biologically inactive phospholipid, contributes to RAF1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Gao,Yijie Liu,Yuancheng Li,Hao Fan,Rong Wu,Rukang Zhang,Brandon Faubert,Yu-Ying He,Marc B Bissonnette,Siyuan Xia,Dong Chen,Hui Mao,Titus J Boggon,Jing Chen

Archives of Iranian medicine 23:688-696 PubMed33107310

2020

Functional Analysis of S2486G Mutation and its Contribution to Pathogenesis of Ankylosing Spondylitis.

Applications

Unspecified application

Species

Unspecified reactive species

Elika Esmaeilzadeh-Gharehdaghi,Ehsan Razmara,Amirreza Bitaraf,Ahmadreza Jamshidi,Mahdi Mahmoudi,Masoud Garshasbi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Lp-PLA2 ELISA Kit

Sandwich ELISA - Human Lp-PLA2 ELISA Kit (AB235643)

Sandwich ELISA - Human Lp-PLA2 ELISA Kit (AB235643)

Sandwich ELISA - Human Lp-PLA2 ELISA Kit (AB235643)

Sandwich ELISA - Human Lp-PLA2 ELISA Kit (AB235643)

Sandwich ELISA - Human Lp-PLA2 ELISA Kit (AB235643)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (4)

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Product promise