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AB260066

Human LRG ELISA Kit (LRG1)

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(1 Publication)

Human LRG ELISA Kit (LRG1) is a single-wash 90-min Simplestep used to quantify Human LRG (LRG1) with a sensitivity of 11.08 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

LRG, LRG1, Leucine-rich alpha-2-glycoprotein

3 Images
ELISA - Human LRG ELISA Kit (LRG1) (AB260066)
  • ELISA

Supplier Data

ELISA - Human LRG ELISA Kit (LRG1) (AB260066)
Sandwich ELISA - Human LRG ELISA Kit (LRG1) (AB260066)
  • sELISA

Supplier Data

Sandwich ELISA - Human LRG ELISA Kit (LRG1) (AB260066)

Interpolated concentrations of native LRG in human serum, plasma (citrate), and U937 cell culture supernatant samples.

The concentrations of LRG were measured in duplicates, interpolated from the LRG standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 10,000 and plasma (citrate) 1 : 5,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LRG concentration was determined to be 17.50 μg/mL in serum and 10.52 μg/mL in plasma (citrate).

Sandwich ELISA - Human LRG ELISA Kit (LRG1) (AB260066)
  • sELISA

Supplier Data

Sandwich ELISA - Human LRG ELISA Kit (LRG1) (AB260066)

Example of human LRG standard curve in Sample Diluent NS.

The LRG standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 11.08 pg/mL

Range

46.88 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human LRG ELISA Kit (LRG1) ab260066 is a rapid single-wash 90-min Sandwich ELISA to measure Human LRG (LRG1)in cell culture supernatant, citrate plasma, serum. This SimpleStep sensitivity is 11.08 pg/mL.

How the assay works

Human LRG (LRG1)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human LRG ELISA Kit (LRG1) ab260066 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

LRG (LRG1) is a leucine-rich repeat glycoprotein present in plasma during granulocyte differentiation. LRG is involved in neovascularization by modulation of TGFbeta signaling in endothelial cells. Elevated levels of LRG are seen in inflammation and in rheumatic diseases like rheumatoid arthritis, adult-onset Still's disease, and Kawaski disease.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": null, "average": "= 86" }, { "sample": "Serum", "range": null, "average": "= 92" }, { "sample": "Citrate plasma", "range": null, "average": "= 95" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information LRG1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncogenesis 13:21 PubMed38871685

2024

Activated platelet-derived exosomal LRG1 promotes multiple myeloma cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Gao,Hang Dong,Siyi Jiang,Fangping Chen,Yunfeng Fu,Yanwei Luo
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com