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AB325456

Human LRG (LRG1) ELISA Kit, Chemiluminescent

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Human LRG (LRG1) ELISA Kit, Chemiluminescent is a 55-min single-wash Simplestep IgniteTM ELISA for the rapid quantification of Human LRG (LRG1) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range :9.1 - 20000 pg/mL with a sensitivity of 3.2 pg/mL
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

LRG, LRG1, Leucine-rich alpha-2-glycoprotein

7 Images
Sandwich ELISA - Human LRG (LRG1) ELISA Kit, Chemiluminescent (AB325456)
  • sELISA

Lab

Sandwich ELISA - Human LRG (LRG1) ELISA Kit, Chemiluminescent (AB325456)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human LRG (LRG1) ELISA Kit, Chemiluminescent (AB325456)
  • sELISA

Lab

Sandwich ELISA - Human LRG (LRG1) ELISA Kit, Chemiluminescent (AB325456)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LRG concentration was determined to be 18.2 µg/mL with a range of 10.8 – 41.5 µg/mL.

Sandwich ELISA - Human LRG (LRG1) ELISA Kit, Chemiluminescent (AB325456)
  • sELISA

Lab

Sandwich ELISA - Human LRG (LRG1) ELISA Kit, Chemiluminescent (AB325456)

Interpolated concentration of native LRG was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 1 : 10,000 serum and 1 : 5,000 plasma (citrate). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human LRG (LRG1) ELISA Kit, Chemiluminescent (AB325456)
  • sELISA

Lab

Sandwich ELISA - Human LRG (LRG1) ELISA Kit, Chemiluminescent (AB325456)

Dynamic range comparison between the Chemiluminescent Human LRG SimpleStep ELISA, colorimetric Human LRG SimpleStep ELISA (ab260066), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and dynamic range.

Sandwich ELISA - Human LRG (LRG1) ELISA Kit, Chemiluminescent (AB325456)
  • sELISA

Lab

Sandwich ELISA - Human LRG (LRG1) ELISA Kit, Chemiluminescent (AB325456)

Interpolated concentration of native LRG was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 25% U937 cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human LRG (LRG1) ELISA Kit, Chemiluminescent (AB325456)
  • sELISA

Lab

Sandwich ELISA - Human LRG (LRG1) ELISA Kit, Chemiluminescent (AB325456)

Standard curve comparison between the Chemiluminescent Human LRG SimpleStep ELISA and colorimetric Human LRG SimpleStep ELISA (ab260066) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human LRG (LRG1) ELISA Kit, Chemiluminescent (AB325456)
  • sELISA

Lab

Sandwich ELISA - Human LRG (LRG1) ELISA Kit, Chemiluminescent (AB325456)

Serum of five individual Rheumatoid Arthritis donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LRG concentration was determined to be 21.4 µg/mL with a range of 18.6 – 24.6 µg/mL.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 3.2 pg/mL

Range

9.1 - 20000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human LRG (LRG1) ELISA Kit, Chemiluminescent (ab325456) is a rapid single-wash sandwich 55-min ELISA to measure Human LRG (LRG1) in Serum, Citrate Plasma, Cell Culture Supernatant with a sensitivity of 3.2 pg/ml.

How the assay works

Human LRG (LRG1) ELISA Kit, Chemiluminescent (ab325456) SimpleStep ELISA®  employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time.  By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

Please refer to our protocol booklet for more details.

Human LRG (LRG1) (ab325456) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature

2. Wash

3. Add prepared ChemiHRP development solution - incubate for 2 min

4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-LRG1 antibody [EPR22432-122] - BSA and Azide free (Capture) ab259551

- Anti-LRG1 antibody [EPR22432-40] - BSA and Azide free (Detector) ab259741

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.4" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "= 92" }, { "sample": "Citrate plasma", "range": null, "average": "= 95" }, { "sample": "Cell culture supernatant", "range": null, "average": "= 86" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information LRG1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com