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AB267624

Human LRIG3 ELISA Kit

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Human LRIG3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human LRIG3 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

LIG3, UNQ287/PRO326/PRO335, LRIG3, Leucine-rich repeats and immunoglobulin-like domains protein 3, LIG-3

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Sandwich ELISA - Human LRIG3 ELISA Kit (AB267624)
  • sELISA

Supplier Data

Sandwich ELISA - Human LRIG3 ELISA Kit (AB267624)

Human LRIG3 ELISA kit standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.4 ng/mL

Range

2.4 - 800 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human LRIG3 ELISA Kit is designed for the quantitative determination of LRIG3 in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human LRIG3 coated on a 96-well plate. Standards and samples are pipetted into the wells and LRIG3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human LRIG3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LRIG3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA antibody pair detects Human LRIG3. Other species not determined.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "75 - 132 %", "average": "= 106.5" }, { "sample": "Plasma", "range": "72 - 89 %", "average": "= 83.8" }, { "sample": "Cell culture media", "range": "136 - 146 %", "average": "= 142.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267624-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267624-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human LRIG3 coated Microplate (12 x 8 wells)", "number":"AB267624-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267624-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267624-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human LRIG3 Detection Antibody", "number":"AB267624-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB267624-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"900X HRP-Streptavidin Concentrate", "number":"AB267624-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB267624-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human LRIG3 Standard (Lyophilized)", "number":"AB267624-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

May play a role in craniofacial and inner ear morphogenesis during embryonic development. May act within the otic vesicle epithelium to control formation of the lateral semicircular canal in the inner ear, possibly by restricting the expression of NTN1 (By similarity).
See full target information LRIG3
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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