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AB278128

Human LRP1 ELISA Kit

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Human LRP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human LRP1 with a sensitivity of 30.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD91, A2MR, APR, LRP1, Prolow-density lipoprotein receptor-related protein 1, LRP-1, Alpha-2-macroglobulin receptor, Apolipoprotein E receptor, APOER

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ELISA - Human LRP1 ELISA Kit (AB278128)
  • ELISA

Supplier Data

ELISA - Human LRP1 ELISA Kit (AB278128)
Sandwich ELISA - Human LRP1 ELISA Kit (AB278128)
  • sELISA

Lab

Sandwich ELISA - Human LRP1 ELISA Kit (AB278128)

Example of human LRP1 standard curve in Sample Diluent NS.

The LRP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human LRP1 ELISA Kit (AB278128)
  • sELISA

Supplier Data

Sandwich ELISA - Human LRP1 ELISA Kit (AB278128)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LRP1 concentration was determined to be 112.6 ng/mL with a range of 66.7 – 236.6 ng/mL.

Sandwich ELISA - Human LRP1 ELISA Kit (AB278128)
  • sELISA

Supplier Data

Sandwich ELISA - Human LRP1 ELISA Kit (AB278128)

Interpolated concentrations of native LRP1 in mouse serum and rat serum samples.

The concentrations of LRP1 were measured in duplicates, interpolated from the LRP1 standard curves and corrected for sample dilution. Undiluted samples are as follows : mouse serum 2.5% and rat serum 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LRP1 concentration was determined to be 199.4 ng/mL in mouse serum and 104.9 ng/mL in rat serum.

Sandwich ELISA - Human LRP1 ELISA Kit (AB278128)
  • sELISA

Supplier Data

Sandwich ELISA - Human LRP1 ELISA Kit (AB278128)

Interpolated concentrations of native LRP1 in human serum, human plasma, and mouse cell culture supernatant samples.

The concentrations of LRP1 were measured in duplicates, interpolated from the LRP1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5%, plasma (citrate) 5%, and RAW264.7 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LRP1 concentration was determined to be 90.0 ng/mL in serum, 72.0 ng/mL in plasma (citrate) and 29.7 ng/mL in RAW264.7 cell culture supernatant.

Sandwich ELISA - Human LRP1 ELISA Kit (AB278128)
  • sELISA

Supplier Data

Sandwich ELISA - Human LRP1 ELISA Kit (AB278128)

Interpolated concentrations of native LRP1 in human HepG2 and Kidney extract samples and samples based on a 200 µg/mL and 400 μg/mL extract load (respectively).

The concentrations of LRP1 were measured in duplicate and interpolated from the LRP1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LRP1 concentration was determined to be 3,095 pg/mL in HepG2 extract and 2,662 pg/mL in Kidney extract.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum, Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 30.6 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human LRP1 ELISA Kit ab278128 is a rapid single-wash 90-min Sandwich ELISA to measure Human LRP1 in cell culture supernatant, cell lysate, serum, tissue extracts. This SimpleStep sensitivity is 30.6 pg/mL.

How the assay works

Human LRP1 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human LRP1 ELISA Kit ab278128 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-LRP1 antibody [EPR22888-223] - BSA and Azide free (Capture) ab281547
- Anti-LRP1 antibody [EPR22888-303] - BSA and Azide free (Detector) ab281547

Prolow-density lipoprotein receptor-related protein 1 (LRP1) is an endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. LRP1 is widely expressed on many cell types including neurons, adipocytes, hepatocytes, vascular smooth muscle cells, fibroblasts, keratinocytes, macrophages, and megakaryocytes. Human LRP1 is an N-glycosylated and sialylated molecule that is cleaved in the Golgi apparatus to produce an 85 kDa transmembrane beta chain and a 515 kDa alpha chain that associates noncovalently with the beta chain but does not itself cross the membrane. The provided protein standard for ab278128 is a fragment of the extracellular domain.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab278128-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human LRP1 Capture Antibody", "number":"AB278128-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CP2", "number":"AB278128-CMP14", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB278128-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human LRP1 Lyophilized Recombinant Protein", "number":"AB278128-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB278128-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB278128-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB278128-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB278128-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB278128-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB278128-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LRP1 Detector Antibody", "number":"AB278128-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB278128-CMP13", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells (PubMed : 11907044, PubMed : 12713657). Required for early embryonic development (By similarity). Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. Acts as an LRPAP1 alpha-2-macroglobulin receptor (PubMed : 1702392, PubMed : 26142438). Acts as TAU/MAPT receptor and controls the endocytosis of TAU/MAPT as well as its subsequent spread (PubMed : 32296178). May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission (PubMed : 12888553). Acts also as a receptor for IGFBP3 to mediate cell growth inhibition (PubMed : 9252371).. (Microbial infection) Functions as a receptor for Pseudomonas aeruginosa exotoxin A.
See full target information LRP1
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websiteProtocolBooklet
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