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AB309401

Human LSECtin ELISA Kit

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Human LSECtin ELISA Kit is a Sandwich ELISA for the measurement of Human LSECtin in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

UNQ431/PRO792, CLEC4G, C-type lectin domain family 4 member G, Liver and lymph node sinusoidal endothelial cell C-type lectin, LSECtin

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Sandwich ELISA - Human LSECtin ELISA Kit (AB309401)
  • sELISA

Supplier Data

Sandwich ELISA - Human LSECtin ELISA Kit (AB309401)

Example of standard curve using ab309401. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.078 ng/mL

Range

0.078 - 20 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB181196

Anti-LSECtin antibody [EPR13724]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] (AB181196)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human LSECtin ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human LSECtin in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human LSECtin coated on a 96-well plate.
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Recovery

[ { "sample": "Plasma", "range": "70 - 75 %", "average": "= 73.19" }, { "sample": "Serum", "range": "71 - 80 %", "average": "= 74.38" }, { "sample": "Cell culture media", "range": "98 - 130 %", "average": "= 117.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309401-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Lyophilized Human LSECtin/CLEC4G Protein Standard", "number":"AB309401-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309401-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309401-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB309401-CMP12", "productcode":"AB309401" }, { "size":"1 x 1 Unit", "name":"Human LSECtin/CLEC4G Antibody-coated ELISA Plate", "number":"AB309401-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human LSECtin/CLEC4G Detection Antibody", "number":"AB309401-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309401-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309401-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LSECtin also known as CLEC4G is a C-type lectin-like receptor weighing around 40 kDa. It is expressed mainly on liver sinusoidal endothelial cells (LSECs) but also noted in lymph node and placenta tissues. As a receptor LSECtin plays an important role in recognizing and binding to specific glycans on pathogens facilitating their capture and subsequent immune response.
Biological function summary

LSECtin functions as an important player in immune recognition and clearance of pathogens. It is involved in pathogen binding through interaction with glycans aiding in the modulation of immune responses. LSECtin along with other related receptors like DC-SIGN and CD23 forms part of a complex that influences pathogen recognition. It particularly interacts with viruses like HIV and Ebola impacting how these pathogens are detected and processed by the immune system.

Pathways

The function of LSECtin integrates into immune system pathways where it influences pathogen clearance. One notable pathway is the pathogen recognition pathway. LSECtin interacts with the liver's immune regulatory network linking with proteins like DC-SIGN that play a role in modulating the immune response. Another pathway it affects is the cell adhesion pathway which involves inflammation regulation.

LSECtin is linked to infections and immune-related liver conditions. In viral infections such as Ebola and HIV LSECtin mediates pathogen capture affecting the body's ability to handle these infections. The receptor's interaction with liver-specific proteins is noted in liver fibrosis where LSECtin and related proteins like CD23 may alter immune function and disease progression. This close relationship highlights the importance of LSECtin in both immune defense and disease pathology.
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Product protocols

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Target data

Binds mannose, N-acetylglucosamine (GlcNAc) and fucose, but not galactose, in a Ca(2+)-dependent manner, in vitro.. (Microbial infection) Acts as a receptor for Japanese encephalitis virus.. (Microbial infection) Acts as a receptor for Ebolavirus.. (Microbial infection) Acts as a receptor for SARS-CoV.. (Microbial infection) Acts as a receptor for Lassa virus and Lymphocytic choriomeningitis virus glycoprotein (PubMed : 22156524, PubMed : 22673088).
See full target information CLEC4G
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websiteProtocolBooklet
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Product promise

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