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Human LSECtin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human LSECtin in Human in Cell culture supernatant, Plasma, Serum samples.

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Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.078 - 20 ng/mL

Sensitivity

= 0.078 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Binds mannose, N-acetylglucosamine (GlcNAc) and fucose, but not galactose, in a Ca(2+)-dependent manner, in vitro.(Microbial infection) Acts as a receptor for Japanese encephalitis virus.(Microbial infection) Acts as a receptor for Ebolavirus.(Microbial infection) Acts as a receptor for SARS-CoV.(Microbial infection) Acts as a receptor for Lassa virus and Lymphocytic choriomeningitis virus glycoprotein (PubMed:22156524, PubMed:22673088).

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
5X Assay Diluent (Item E2)
1 x 15 mL
Biotinylated Human LSECtin/CLEC4G Detection Antibody
2 x 1 Vial
Human LSECtin/CLEC4G Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human LSECtin/CLEC4G Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

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Human LSECtin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human LSECtin in Human in Cell culture supernatant, Plasma, Serum samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.078 - 20 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 0.078 ng/mL

Recovery

Sample specific recovery

Sample type

Plasma

Average %

= 73.19

Range

70 - 75 %

Sample type

Serum

Average %

= 74.38

Range

71 - 80 %

Sample type

Cell culture media

Average %

= 117.1

Range

98 - 130 %

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Human LSECtin ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human LSECtin in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human LSECtin coated on a 96-well plate.

Supplementary info

Biological function summary

LSECtin functions as an important player in immune recognition and clearance of pathogens. It is involved in pathogen binding through interaction with glycans aiding in the modulation of immune responses. LSECtin along with other related receptors like DC-SIGN and CD23 forms part of a complex that influences pathogen recognition. It particularly interacts with viruses like HIV and Ebola impacting how these pathogens are detected and processed by the immune system.

Activity summary

LSECtin also known as CLEC4G is a C-type lectin-like receptor weighing around 40 kDa. It is expressed mainly on liver sinusoidal endothelial cells (LSECs) but also noted in lymph node and placenta tissues. As a receptor LSECtin plays an important role in recognizing and binding to specific glycans on pathogens facilitating their capture and subsequent immune response.

Pathways

The function of LSECtin integrates into immune system pathways where it influences pathogen clearance. One notable pathway is the pathogen recognition pathway. LSECtin interacts with the liver's immune regulatory network linking with proteins like DC-SIGN that play a role in modulating the immune response. Another pathway it affects is the cell adhesion pathway which involves inflammation regulation.

Associated diseases and disorders

LSECtin is linked to infections and immune-related liver conditions. In viral infections such as Ebola and HIV LSECtin mediates pathogen capture affecting the body's ability to handle these infections. The receptor's interaction with liver-specific proteins is noted in liver fibrosis where LSECtin and related proteins like CD23 may alter immune function and disease progression. This close relationship highlights the importance of LSECtin in both immune defense and disease pathology.

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Terms & Conditions.

1 product image

  • Sandwich ELISA - Human LSECtin ELISA Kit (ab309401), expandable thumbnail

    Sandwich ELISA - Human LSECtin ELISA Kit (ab309401)

    Example of standard curve using ab309401. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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