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AB313988

Human LTBP2 ELISA Kit

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Human LTBP2 ELISA Kit is a sandwich ELISA designed to quantify Human LTBP2 with a sensitivity of 17.5 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 17.5 - 5000 pg/mL

View Alternative Names

C14orf141, LTBP3, LTBP2, Latent-transforming growth factor beta-binding protein 2, LTBP-2

1 Images
Sandwich ELISA - Human LTBP2 ELISA Kit (AB313988)
  • sELISA

Supplier Data

Sandwich ELISA - Human LTBP2 ELISA Kit (AB313988)

Example of standard curve using ab313988. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 17.5 pg/mL

Range

17.5 - 5000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human LTBP2 ELISA Kit ab313988 is a sandwich ELISA to measure Human LTBP2 in serum, plasma, cell culture supernatant with a sensitivity of 17.5 pg/ml.

How the assay works

This assay employs an antibody specific for human LTBP2 coated on a 96-well plate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human LTBP2 ELISA Kit ab313988 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.
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Recovery

[ { "sample": "Serum", "range": "101 - 123 %", "average": "= 112.5" }, { "sample": "Plasma", "range": "98 - 120 %", "average": "= 107.3" }, { "sample": "Cell culture media", "range": "93 - 105 %", "average": "= 100.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313988-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313988-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human LTBP2 Protein Standard", "number":"AB313988-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human LTBP2 Antibody-coated ELISA Plate", "number":"AB313988-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human LTBP2 Detection Antibody", "number":"AB313988-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313988-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313988-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313988-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB313988-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LTBP2 also known as latent transforming growth factor-beta binding protein 2 is a glycoprotein involved in the regulation of transforming growth factor-beta (TGF-β) signaling. This protein has a mass of approximately 195 kDa. LTBP2 is expressed in a variety of tissues including the lungs heart and eyes. It plays an important role in the extracellular matrix where it is fundamental to the sequestration and subsequent activation of TGF-β.
Biological function summary

LTBP2 interacts with the extracellular matrix components and acts as a structural protein. LTBP2 is an important part of TGF-β complexes. By anchoring TGF-β in a latent form it controls the availability and activation of TGF-β which is necessary for processes like cell growth differentiation and tissue repair. Without LTBP2 the controlled release of TGF-β can be disrupted impacting various cell functions.

Pathways

LTBP2 regulates the TGF-β signaling pathway and maintains its equilibrium. This regulatory function links LTBP2 to the pathways involving cell proliferation and differentiation. LTBP2 closely interacts with other members of the LTBP family as well as proteins like fibrillin which are involved in the structural integrity of the extracellular matrix. These interactions are significant for maintaining proper signal transduction and cellular responses.

LTBP2 mutations have associations with conditions such as glaucoma and Weill-Marchesani syndrome. In glaucoma abnormalities in LTBP2 might affect the integrity of the extracellular matrix influencing intraocular pressure regulation. It also connects to fibrillin-related disorders due to its interaction with fibrillin proteins. These associations highlight the importance of LTBP2 in both structural and signaling roles within the body.
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Product protocols

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Target data

May play an integral structural role in elastic-fiber architectural organization and/or assembly.
See full target information LTBP2
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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