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AB309396

Human LTBR ELISA Kit

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Human LTBR ELISA Kit is a Sandwich ELISA for the measurement of Human LTBR in Human in Cell Culture Media, Biofluids samples.
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Sandwich ELISA - Human LTBR ELISA Kit (AB309396)
  • sELISA

Supplier Data

Sandwich ELISA - Human LTBR ELISA Kit (AB309396)

Example of standard curve using ab309396. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4.5 pg/mL

Range

4.5 - 1000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB314571

Anti-LTBR antibody [EPR26646-39] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-LTBR antibody [EPR26646-39] - BSA and Azide free (Detector) (AB314571)

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Assay Kits

AB314569

Human LTBR Antibody Pair - BSA and Azide free

RabMAb

Sandwich ELISA - Human LTBR Antibody Pair - BSA and Azide free (AB314569)

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Proteins & Peptides

AB198752

Recombinant Mouse LTBR protein (Fc tag C-Terminus)

SDS-PAGE - Recombinant Mouse LTBR protein (Fc tag C-Terminus) (AB198752)

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Biochemicals

AB120163

SB431542, ALK inhibitor

Chemical Structure - SB431542, ALK inhibitor (AB120163)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human LTBR ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human LTBR in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human LTBR coated on a 96-well plate.
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Recovery

[ { "sample": "Cell culture media", "range": "96 - 104 %", "average": "= 98.86" }, { "sample": "Plasma", "range": "102 - 124 %", "average": "= 113.2" }, { "sample": "Serum", "range": "110 - 115 %", "average": "= 111.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309396-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309396-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Lymphotoxin beta R/TNF RIII/TNFRSF3 Protein Standard", "number":"AB309396-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB309396-CMP11", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309396-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309396-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Lymphotoxin beta R/TNF RIII/TNFRSF3 Antibody-coated ELISA Plate", "number":"AB309396-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Lymphotoxin beta R/TNF RIII/TNFRSF3 Detection Antibody", "number":"AB309396-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309396-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LTBR (lymphotoxin beta receptor) also known as the TNFRSF3 is a significant protein that functions as a receptor for lymphotoxin and other related cytokines. The protein has a mass of around 62 kDa and is expressed in various tissues including lymphoid tissues and the epithelial cells of several organs. LTBR is present as a membrane-bound protein and helps mediate signaling that is important for cellular communication and function. Key interactions with ligands such as LTA and LIGHT activate signaling pathways which play roles in immune response.
Biological function summary

LTBR coordinates multiple cellular processes especially in immune regulation and homeostasis. It forms a complex with cytokines that binds to its extracellular domain triggering downstream signaling cascades. These cascades lead to activation of nuclear factor kappa B (NF-kB) which controls gene transcription and promotes survival and differentiation of immune cells. The capability of LTBR to interact with other TNF receptors expands its influence in lymphoid organogenesis and maintenance especially in maintaining the integrity of the lymphoid system.

Pathways

LTBR significantly participates in both the NF-kB and the non-canonical NF-kB pathways. These pathways are essential for regulating immune response and inflammation. Within these pathways LTBR interacts with other proteins such as TRAF3 and TRAF2 to mediate signaling processes. This arrangement allows for modulation of immune responses and is critical for recognizing and eliminating pathogens. Additionally the involvement of LTBR in immune system pathways aligns it with the functions of closely related proteins like TNF receptor superfamily members.

LTBR has been implicated in autoimmune diseases and cancer. In autoimmunity aberrant signaling through LTBR may lead to inappropriate immune responses and inflammation as seen in disorders like systemic lupus erythematosus. Moreover in oncology abnormalities in LTBR signaling can influence tumor microenvironments and support tumor cell survival. Throughout these disease contexts LTBR often interacts with other risk-associated proteins such as NFKB1 highlighting its integration in complex pathological processes.
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Product protocols

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Target data

Receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT (PubMed : 24248355). Activates NF-kappa-B signaling pathway upon stimulation with lymphotoxin (LTA(1)-LTB(2)) (PubMed : 24248355). Promotes apoptosis via TRAF3 and TRAF5. May play a role in the development of lymphoid organs.
See full target information LTBR

Alternative Names

D12S370, TNFCR, TNFR3, TNFRSF3, LTBR, Tumor necrosis factor receptor superfamily member 3, Lymphotoxin-beta receptor, Tumor necrosis factor C receptor, Tumor necrosis factor receptor 2-related protein, Tumor necrosis factor receptor type III, TNF-RIII, TNFR-III
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websiteProtocolBooklet
en
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Product promise

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