Human Lumican ELISA Kit

Product details

The Human Lumican Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213809) is designed for the quantitative detection of Human Lumican in cell culture supernatants, serum and plasma (heparin).

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A polyclonal antibody from goat specific for Lumican has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: Q19-N338) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Lumican is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Lumican amount of sample captured in plate.

Lumican, also known as LUM, is a protein which in humans is encoded by the LUM gene. This gene is a member of the small interstitial proteoglycan gene (SIPG) family, and it is a keratan sulfate proteoglycan which presents large quantities in the corneal stroma and in interstitial collagenous matrices of the heart, aorta, skeletal muscle, skin and intervertebral discs. Lumican is mapped to 12q21.33. It interacts with collagen and limits growth of fibrils in diameter. In the cornea, Lumican not only interacts with collagen molecules to limit fibril growth, but also plays a critical role in the regular spacing of fibrils and acquisition of corneal transparency by virtue of its keratan sulfate-containing glycosaminoglycan side chains LDC.