AB315054

Human Lumican ELISA Kit

SimpleStep
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Human Lumican ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Lumican with a sensitivity of 20.177 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

LDC, SLRR2D, LUM, Lumican, Keratan sulfate proteoglycan lumican, KSPG lumican

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Sandwich ELISA - Human Lumican ELISA Kit (AB315054)

sELISA

Supplier Data

Sandwich ELISA - Human Lumican ELISA Kit (AB315054)

Interpolated concentration of native Lumican was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Saos-2 cell culture supernatant 1 : 50 and synovial fluid 1 : 333. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Lumican ELISA Kit (AB315054)

Interpolated concentration of native Lumican was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 2,000 serum, plasma(citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Lumican ELISA Kit (AB315054)

Example of human Lumican standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Lumican ELISA Kit (AB315054)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lumican concentration was determined to be 4,360 ng/mL with a range of 2,394 – 7,133 ng/mL.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Cell culture supernatant, Citrate plasma, Heparin Plasma, Other biological fluids

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 20.177 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Lumican SimpleStep ELISA^® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Lumican protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human Lumican with 20.177 pg/ml sensitivity.

SimpleStep ELISA^® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details. Our SimpleStep ELISA^® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA^® microplate (ab315054) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA^® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.2" } ]

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Recovery

[ { "sample": "Serum", "range": "72 - 107 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "109 - 123 %", "average": "= 117" }, { "sample": "EDTA Plasma", "range": "78 - 100 %", "average": "= 90" }, { "sample": "Heparin Plasma", "range": "88 - 119 %", "average": "= 106" }, { "sample": "Cell culture supernatant", "range": "80 - 110 %", "average": "= 97" }, { "sample": "Other biological fluids", "range": "92 - 116 %", "average": "= 108" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

See full target information LUM

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Lumican ELISA Kit

Sandwich ELISA - Human Lumican ELISA Kit (AB315054)

Sandwich ELISA - Human Lumican ELISA Kit (AB315054)

Sandwich ELISA - Human Lumican ELISA Kit (AB315054)

Sandwich ELISA - Human Lumican ELISA Kit (AB315054)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Complementary Products

Proteins & Peptides

Primary Antibodies

Assay Kits

Assay Kits

Product promise