AB178658
Human Luteinizing hormone ELISA Kit (LH)
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(2 Publications)
Human Luteinizing hormone ELISA Kit (LH) is a sandwich ELISA designed to quantify Human Luteinizing hormone (LH) with a sensitivity of 0.22 mU/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 5 - 200 mU/mL
View Alternative Names
Lutropin subunit beta, Lutropin beta chain, Luteinizing hormone subunit beta, LH-B, LSH-B, LSH-beta, LHB
1 Images
- sELISA
Supplier Data
Sandwich ELISA - Human Luteinizing hormone ELISA Kit (LH) (AB178658)
Representative standard curve using ab178658
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
Product details
Human Luteinizing hormone ELISA Kit (LH) ab178658 is a sandwich ELISA to measure Human Luteinizing hormone (LH) in serum, citrate plasma, heparin plasma with a sensitivity of 0.22 0.
How the assay works
A 96-well plate has been precoated with Streptavidin. Samples, standards and the LH-HRP and Bitoin conjugate are added to the wells. Biotinylated monoclonal and enzyme labeled antibodies are added and the reactants mixed: these antibodies have high affinity and specificity and are directed against distinct and different epitopes of LH. Reaction between the various LH antibodies and native LH occurs in the microwells without competition or steric hindrance forming a soluble sandwich complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of LH in the sample and the intensity is measured at 450 nm.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human Luteinizing hormone ELISA Kit (LH) ab178658 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Add prepared Conjugation Solution to appropriate wells. Incubate at room temperature.
3. After washing, add TMB Substrate Solution to each well. Incubate at room temperature.
4. Add Stop Solution. Read at 450 nm.
How the assay works
A 96-well plate has been precoated with Streptavidin. Samples, standards and the LH-HRP and Bitoin conjugate are added to the wells. Biotinylated monoclonal and enzyme labeled antibodies are added and the reactants mixed: these antibodies have high affinity and specificity and are directed against distinct and different epitopes of LH. Reaction between the various LH antibodies and native LH occurs in the microwells without competition or steric hindrance forming a soluble sandwich complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of LH in the sample and the intensity is measured at 450 nm.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human Luteinizing hormone ELISA Kit (LH) ab178658 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Add prepared Conjugation Solution to appropriate wells. Incubate at room temperature.
3. After washing, add TMB Substrate Solution to each well. Incubate at room temperature.
4. Add Stop Solution. Read at 450 nm.
Precision
[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "<= 9.21" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 15, "mean": null, "standardDeviation": null, "coefficientOfVariability": "<= 7.91" } ]
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
Publications (2)
Recent publications for all applications. Explore the full list and refine your search
American journal of translational research 13:5350-5355 PubMed34150129
2021
Evaluation of diagnosis and prognosis with anti-mullerian hormone level in early missed abortion.
Applications
Unspecified application
Species
Unspecified reactive species
Juan Xie,Xuan Zhou,Lin Fang,Jun Xiong,Xiaoling Tao
International journal of fertility & sterility 12:37-42 PubMed29334205
2018
Overexpression of Endometrial Estrogen Receptor-Alpha in The Window of Implantation in Women with Unexplained Infertility.
Applications
Unspecified application
Species
Unspecified reactive species
Mehran Dorostghoal,Hamid-O-Allah Ghaffari,Farideh Marmazi,Narjes Keikhah
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