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AB313989

Human LXN/TCI ELISA Kit

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Human LXN/TCI ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human LXN/TCI in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Latexin, Endogenous carboxypeptidase inhibitor, Protein MUM, Tissue carboxypeptidase inhibitor, ECI, TCI, LXN

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Sandwich ELISA - Human LXN/TCI ELISA Kit (AB313989)
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Supplier Data

Sandwich ELISA - Human LXN/TCI ELISA Kit (AB313989)

Example of standard curve using ab313989. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.07 ng/mL

Range

0.07 - 15 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human LXN/TCI ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human LXN/TCI in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human LXN/TCI coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "69 - 132 %", "average": "= 85.41" }, { "sample": "Plasma", "range": "71 - 78 %", "average": "= 74.59" }, { "sample": "Cell culture media", "range": "78 - 126 %", "average": "= 102.7" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LXN also known as Latexin or TCI (Tryptase Inhibitor Complex) is a protein with a molecular mass of approximately 24 kDa. LXN acts as an inhibitor of carboxypeptidase A and is primarily involved in regulating protease activity. It is expressed mainly in the liver lung and kidney playing a significant role in protein degradation processes. LXN's inhibitory functions contribute significantly to cellular processes by modulating enzyme activity.
Biological function summary

LXN influences cell proliferation and differentiation. It participates in cellular protection mechanisms against enzymatic damage and balances protease and anti-protease activities in tissues. Although not part of a large complex LXN impacts various cellular events through its protease inhibitor function making it important in tissue homeostasis. Studies suggest its importance in maintaining cell viability and regulating cellular stress responses.

Pathways

LXN associates with pathways linked to inflammation and tissue remodeling with particular emphasis on the protease-antiprotease balance. LXN acts within the kallikrein-kinin system and relates to proteins such as carboxypeptidase A1 which it directly inhibits. Its modulation of these pathways is essential for normal physiological responses and protective mechanisms against tissue damage.

LXN has been linked to cancer and inflammation-related conditions. In cancer its regulation of protease activity can influence tumor growth and metastasis often interacting with proteins like tryptase. LXN's role in inflammation relates to conditions such as rheumatoid arthritis where its influence on the balance of proteases and inhibitors impacts tissue damage and repair processes. Further research into LXN's roles may help elucidate mechanisms driving these diseases offering potential therapeutic targets.
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Product protocols

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Target data

Hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4. May play a role in inflammation.
See full target information LXN
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB154744

Anti-LXN/TCI antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXN/TCI antibody (AB154744)

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Cell Lines & Lysates

AB265921

Human LXN (TCI) knockout HeLa cell line

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Proteins & Peptides

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Recombinant Human LXN/TCI protein

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Cell Lines & Lysates

AB258948

Human LXN (TCI) knockout HeLa cell lysate

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Product promise

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