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AB267798

Human Lysozyme ELISA Kit

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(3 Publications)

Human Lysozyme ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Lysozyme with a sensitivity of 7.31 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

LZM, LYZ, Lysozyme C

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ELISA - Human Lysozyme ELISA Kit (AB267798)
  • ELISA

Supplier Data

ELISA - Human Lysozyme ELISA Kit (AB267798)
Sandwich ELISA - Human Lysozyme ELISA Kit (AB267798)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lysozyme ELISA Kit (AB267798)

Serum from ten individual healthy human female donors were measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lysozyme concentration was determined to be 3,932.39 ng/mL with a range of 1,520.16 –6,208.68 ng/mL.

Sandwich ELISA - Human Lysozyme ELISA Kit (AB267798)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lysozyme ELISA Kit (AB267798)

Interpolated concentrations of native Lysozyme in human serum, and plasma samples.

The concentrations of Lysozyme were measured in duplicates, interpolated from the Lysozyme standard curves and corrected for sample dilution. Undiluted samples are as follows : 1 : 2,000 serum, 1 : 2,000 plasma (citrate), 1 : 2,000 plasma (heparin) and 1 : 2,000 plasma (edta). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lysozyme concentration was determined to be 2608.00 ng/mL in serum, 2993.07 ng/mL in plasma (citrate) and 2814.88 ng/mL in plasma (heparin) and 2811.86 ng/mL in plasma (edta).

Sandwich ELISA - Human Lysozyme ELISA Kit (AB267798)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lysozyme ELISA Kit (AB267798)

Interpolated concentrations of native Lysozyme in human milk, and saliva samples.

The concentrations of Lysozyme were measured in duplicates, interpolated from the Lysozyme standard curves and corrected for sample dilution. Undiluted samples are as follows : 1 : 100,000 milk, and 1 : 2,000,000 saliva. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lysozyme concentration was determined to be 150.03 μg/mL in milk, 2,696.20 μg/mL in saliva.

Sandwich ELISA - Human Lysozyme ELISA Kit (AB267798)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lysozyme ELISA Kit (AB267798)

Interpolated concentrations of native Lysozyme in human urine, and CSF samples.

The concentrations of Lysozyme were measured in duplicates, interpolated from the Lysozyme standard curves and corrected for sample dilution. Undiluted samples are as follows : 1 : 20 urine, 1;100 CSF. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lysozyme concentration was determined to be 23.33 ng/mL in urine, 78.43 ng/mL in CSF.

Sandwich ELISA - Human Lysozyme ELISA Kit (AB267798)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lysozyme ELISA Kit (AB267798)

Example of human Lysozyme standard curve in Sample Diluent.

The Lysozyme standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Heparin Plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 7.31 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Lysozyme ELISA Kit ab267798 is a rapid single-wash 90-min Sandwich ELISA to measure Human Lysozyme in cell culture supernatant, EDTA plasma, heparin plasma, milk, saliva, serum, urine. This SimpleStep sensitivity is 7.31 pg/mL.

How the assay works

Human Lysozyme SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Lysozyme ELISA Kit ab267798 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Lysozyme antibody [EPR22270-53] - BSA and Azide free (Capture) ab269381
- Anti-Lysozyme antibody [EPR22270-47] - BSA and Azide free (Detector) ab269381

Lysozyme is an integral part of the innate immune system where it serves to hydrolyze the1,4-beta linkages of N-acetylmuramic acid and N-acetyl-D-glucosamine. This hydrolysis helps weaken the cell membrane of gram-positive bacteria. It is found in high abundance in secretory fluids, i.e tears, saliva, milk.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.7" } ]
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Recovery

[ { "sample": "Serum", "range": "99 - 107 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "107 - 112 %", "average": "= 105" }, { "sample": "Urine", "range": "107 - 116 %", "average": "= 111" }, { "sample": "Heparin Plasma", "range": "107 - 115 %", "average": "= 110" }, { "sample": "Citrate plasma", "range": "113 - 117 %", "average": "= 115" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lysozyme also known as muramidase is a protein with enzymatic activity that breaks down bacterial cell walls by hydrolyzing the glycosidic bonds of peptidoglycan. The molecular weight of lysozyme is approximately 14 kDa. This protein is expressed in various tissues and body fluids including egg whites human saliva tears and milk. Lysozyme acts as an antimicrobial agent and plays a significant role in the innate immune system.
Biological function summary

Lysozyme contributes to host defense by targeting bacteria. It is not part of a complex but functions as a single enzyme. By compromising the integrity of bacterial cell walls it clears pathogens swiftly from the body. Lysozyme activity assays measure its ability to hydrolyze bacterial peptidoglycan reflecting its role in immune defense. Many cells such as neutrophils and macrophages express lysozyme during the immune response aiding in pathogen clearance.

Pathways

Lysozyme is chiefly involved in the antibacterial humoral response pathway. Its action aligns with other antimicrobial proteins such as lactoferrin. Both lysozyme and lactoferrin are integral to the body’s first line of defense against bacterial invasion. In addition lysozyme is relevant in the pathway managing mucosal immunity working in conjunction with proteins that enhance pathogen clearance like defensins.

Lysozyme has links to conditions such as sarcoidosis and hereditary renal amyloidosis. In sarcoidosis altered expression patterns of lysozyme may serve as a marker for disease activity. In hereditary renal amyloidosis mutations in the lysozyme gene result in amyloid fibril formation leading to renal dysfunction. Understanding these connections helps in the diagnosis and treatment of related conditions.
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Product protocols

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Target data

Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
See full target information LYZ
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Pediatric gastroenterology, hepatology & nutrition 27:322-331 PubMed39319280

2024

Relationship of MicroRNA according to Immune Components of Breast Milk in Korean Lactating Mothers.

Applications

Unspecified application

Species

Unspecified reactive species

You Jin Choi,Da Hye Lee,Jeonglyn Song,Ki-Uk Kim,Hyeyoung Min,Sung-Hoon Chung,Tae Hyeong Kim,Chae-Young Kim,Insoo Kang,Na Mi Lee,Dae Yong Yi

Scientific reports 14:12879 PubMed38839896

2024

Azathioprine promotes intestinal epithelial cell differentiation into Paneth cells and alleviates ileal Crohn's disease severity.

Applications

Unspecified application

Species

Unspecified reactive species

Mohab Ragab,Heidi Schlichting,Maren Hicken,Patricia Mester,Misa Hirose,Larissa N Almeida,Lea Christiansen,Saleh Ibrahim,Hauke Christian Tews,Senad Divanovic,Christian Sina,Stefanie Derer

Microbiology spectrum 11:e0078023 PubMed37436157

2023

Microbiome Engineering Using Probiotic Yeast: and the Secreted Human Lysozyme Lead to Changes in the Gut Microbiome and Metabolome of Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jungyeon Kim,Christine Atkinson,Michael J Miller,Kyoung Heon Kim,Yong-Su Jin
View all publications
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websiteProtocolBooklet
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