JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB108880

Human Lysozyme ELISA Kit (LZM)

Be the first to review this product! Submit a review

|

(10 Publications)

Human Lysozyme ELISA Kit (LZM) is a Sandwich (quantitative) ELISA for the measurement of Human Lysozyme (LZM) in Human in Biofluids samples.

View Alternative Names

LZM, LYZ, Lysozyme C

2 Images
Sandwich ELISA - Human Lysozyme ELISA Kit (LZM) (AB108880)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lysozyme ELISA Kit (LZM) (AB108880)

Representative Standard Curve using ab108880.

ELISA - Human Lysozyme ELISA Kit (LZM) (AB108880)
  • ELISA

Lab

ELISA - Human Lysozyme ELISA Kit (LZM) (AB108880)

ELISA data.

Human lysozyme measured in biological fluids showing quantity (ng) per mL of tested sample. Human serum and plasma diluted 1000-9000 fold. Human saliva diluted 8000-24000 fold. Human urine diluted 10 fold.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Saliva, Urine, Plasma, Milk, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

>= 46 pg/mL

Range

0.078 - 5 ng/mL

Assay time

4h

Assay Platform

Microplate

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Lysozyme (LZM) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of human Lysozyme in plasma, serum, urine, saliva, CSF and cell culture supernatants.

A Lysozyme specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Lysozyme specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Lysozyme captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.9" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.7" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108880-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108880-CMP06", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108880-CMP09", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Lysozyme Antibody", "number":"AB108880-CMP01", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108880-CMP05", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108880-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Lysozyme Microplate (12 x 8 well strips)", "number":"AB108880-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lysozyme Standard", "number":"AB108880-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108880-CMP02", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108880-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lysozyme also known as muramidase is a protein with enzymatic activity that breaks down bacterial cell walls by hydrolyzing the glycosidic bonds of peptidoglycan. The molecular weight of lysozyme is approximately 14 kDa. This protein is expressed in various tissues and body fluids including egg whites human saliva tears and milk. Lysozyme acts as an antimicrobial agent and plays a significant role in the innate immune system.
Biological function summary

Lysozyme contributes to host defense by targeting bacteria. It is not part of a complex but functions as a single enzyme. By compromising the integrity of bacterial cell walls it clears pathogens swiftly from the body. Lysozyme activity assays measure its ability to hydrolyze bacterial peptidoglycan reflecting its role in immune defense. Many cells such as neutrophils and macrophages express lysozyme during the immune response aiding in pathogen clearance.

Pathways

Lysozyme is chiefly involved in the antibacterial humoral response pathway. Its action aligns with other antimicrobial proteins such as lactoferrin. Both lysozyme and lactoferrin are integral to the body’s first line of defense against bacterial invasion. In addition lysozyme is relevant in the pathway managing mucosal immunity working in conjunction with proteins that enhance pathogen clearance like defensins.

Lysozyme has links to conditions such as sarcoidosis and hereditary renal amyloidosis. In sarcoidosis altered expression patterns of lysozyme may serve as a marker for disease activity. In hereditary renal amyloidosis mutations in the lysozyme gene result in amyloid fibril formation leading to renal dysfunction. Understanding these connections helps in the diagnosis and treatment of related conditions.
style
left-column

Product protocols

style
left-column

Target data

Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
See full target information LYZ
style
left-column

Publications (10)

Recent publications for all applications. Explore the full list and refine your search

PLoS neglected tropical diseases 17:e0011689 PubMed37862368

2023

Longitudinal changes in tear cytokines and antimicrobial proteins in trachomatous disease.

Applications

Unspecified application

Species

Unspecified reactive species

Amber Barton,Nkoyo Faal,Athumani Ramadhani,Tamsyn Derrick,Elias Mafuru,Tara Mtuy,Patrick Massae,Aiweda Malissa,Hassan Joof,Pateh Makalo,Ansumana Sillah,Anna Harte,Harry Pickering,Robin Bailey,David Cw Mabey,Matthew J Burton,Martin J Holland

Scientific reports 13:14327 PubMed37653055

2023

Beyond the effects of HIV infection and integrase inhibitors-based therapies on oral bacteriome.

Applications

Unspecified application

Species

Unspecified reactive species

Pablo Villoslada-Blanco,Patricia Pérez-Matute,Emma Recio-Fernández,María Íñiguez,Pilar Blanco-Navarrete,Luis Metola,Valvanera Ibarra,Jorge Alba,María de Toro,José A Oteo

International journal of molecular sciences 23: PubMed35955814

2022

Possible Implications of Bacteriospermia on the Sperm Quality, Oxidative Characteristics, and Seminal Cytokine Network in Normozoospermic Men.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Tvrdá,Daniel Lovíšek,Eliška Gálová,Marianna Schwarzová,Eva Kováčiková,Simona Kunová,Jana Žiarovská,Miroslava Kačániová

Frontiers in psychology 12:564490 PubMed33841224

2021

Combined Chair-Based Exercises Improve Functional Fitness, Mental Well-Being, Salivary Steroid Balance, and Anti-microbial Activity in Pre-frail Older Women.

Applications

Unspecified application

Species

Unspecified reactive species

Guilherme Eustáquio Furtado,Rubens Vinícius Letieri,Adriana Silva-Caldo,Joice C S Trombeta,Clara Monteiro,Rafael Nogueira Rodrigues,Ana Vieira-Pedrosa,Marcelo Paes Barros,Cláudia Regina Cavaglieri,Eef Hogervorst,Ana Maria Teixeira,José Pedro Ferreira

PloS one 16:e0246393 PubMed33690604

2021

Linking bacterial enterotoxins and alpha defensin 5 expansion in the Crohn's colitis: A new insight into the etiopathogenetic and differentiation triggers driving colonic inflammatory bowel disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tanu Rana,Olga Y Korolkova,Girish Rachakonda,Amanda D Williams,Alexander T Hawkins,Samuel D James,Amos M Sakwe,Nian Hui,Li Wang,Chang Yu,Jeffrey S Goodwin,Michael G Izban,Regina S Offodile,Mary K Washington,Billy R Ballard,Duane T Smoot,Xuan-Zheng Shi,Digna S Forbes,Anil Shanker,Amosy E M'Koma

Journal of dairy science 104:167-178 PubMed33162091

2020

Investigation of donkey milk bacterial diversity by 16S rDNA high-throughput sequencing on a Cyprus donkey farm.

Applications

Unspecified application

Species

Unspecified reactive species

P Papademas,E Kamilari,M Aspri,D A Anagnostopoulos,P Mousikos,A Kamilaris,D Tsaltas

Scientific reports 10:4815 PubMed32179759

2020

Multiplexed MRM-based protein quantification of putative prognostic biomarkers for chronic kidney disease progression in plasma.

Applications

Unspecified application

Species

Unspecified reactive species

Manousos Makridakis,Georgia Kontostathi,Eleni Petra,Rafael Stroggilos,Vasiliki Lygirou,Szymon Filip,Flore Duranton,Harald Mischak,Angel Argiles,Jerome Zoidakis,Antonia Vlahou

Journal of translational medicine 17:68 PubMed30823930

2019

Processed human amniotic fluid retains its antibacterial activity.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Mao,Jan Pierce,Anya Singh-Varma,Michael Boyer,Joachim Kohn,Jo-Anna Reems

Journal of lipid research 59:1424-1432 PubMed29739865

2018

2-Chlorofatty acids: lipid mediators of neutrophil extracellular trap formation.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa N D Palladino,Lalage A Katunga,Grant R Kolar,David A Ford

Nephrology, dialysis, transplantation : official p 30:1842-52 PubMed26160894

2015

New insights in molecular mechanisms involved in chronic kidney disease using high-resolution plasma proteome analysis.

Applications

sELISA

Species

Human

Griet Glorieux,William Mullen,Flore Duranton,Szymon Filip,Nathalie Gayrard,Holger Husi,Eva Schepers,Nathalie Neirynck,Joost P Schanstra,Joachim Jankowski,Harald Mischak,Àngel Argilés,Raymond Vanholder,Antonia Vlahou,Julie Klein
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB200015

Human Lactoferrin ELISA Kit

Recombinant|SimpleStep

ELISA - Human Lactoferrin ELISA Kit (AB200015)

  • 5
  • 1
Assay Kits

AB196263

Human IgA ELISA Kit

Recombinant|SimpleStep

ELISA - Human IgA ELISA Kit (AB196263)

  • 4
  • 1
Assay Kits

AB211113

Lysozyme Activity Assay Kit (Fluorometric)

Functional Studies - Lysozyme Activity Assay Kit (Fluorometric) (AB211113)

  • 0
  • 0
Assay Kits

AB260064

Human C-Peptide ELISA Kit

Recombinant|SimpleStep

ELISA - Human C-Peptide ELISA Kit (AB260064)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com