AB275905

Human LYST ELISA Kit

Recombinant
SimpleStep
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Human LYST ELISA Kit is a single-wash 90-min Simplestep used to quantify Human LYST with a sensitivity of 20 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CHS, CHS1, LYST, Lysosomal-trafficking regulator, Beige homolog

2 Images

Sandwich ELISA - Human LYST ELISA Kit (AB275905)

sELISA

Supplier Data

Sandwich ELISA - Human LYST ELISA Kit (AB275905)

Example of human LYST standard curve in 1X Cell Extraction Buffer PTR.

The LYST standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human LYST ELISA Kit (AB275905)

Interpolated concentrations of native LYST in human THP-1 cell extract based on a 300 μg/mL extract load.

The concentrations of LYST were measured in duplicate and interpolated from the LYST standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LYST concentration was determined to be 3,115.82 pg/mL.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 20 pg/mL

Range

156.25 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human LYST ELISA kit (ab275905) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human LYST protein in human cell extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human LYST with 20 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

LYST, lysosomal trafficking regulator, is a vesicular transport protein. Defects in this protein lead to Chédiak–Higashi syndrome, a lysosomal storage disease that manifests as hypopigmentation, neutropenia, defective white blood cell function, and coagulation defects. The immunogen from which this kit was made represents the BEACH domain of the protein. Although this domain is conserved across many mammalian species, no cross-reactivity was seen.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12.3" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "92 - 110 %", "average": "= 102" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab275905-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB275905-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB275905-CMP15", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB275905-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LYST Capture Antibody", "number":"AB275905-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB275905-CMP14", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human LYST Lyophilized Recombinant Protein", "number":"AB275905-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB275905-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB275905-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB275905-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB275905-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB275905-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LYST Detector Antibody", "number":"AB275905-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LYST protein also known as Lysosomal Trafficking Regulator or Beige is involved in intracellular transport processes. With an approximate molecular mass of 429 kDa it plays a role in the trafficking of lysosomal contents. Expressed in various tissues it particularly affects those with a high demand for lysosomal activity such as immune system cells. As part of its mechanical function LYST regulates the morphology of lysosomes and endosomes by facilitating fusion and movement of these vesicles.

Biological function summary

LYST directly influences the cellular degradation and recycling process. The protein does not act alone but functions as part of a larger cellular machinery that maintains lysosomal homeostasis. Its role is particularly important in immune cell function where it ensures proper lysosomal delivery and secretion especially in cytotoxic T cells and natural killer cells. These activities are important for granulocyte and lymphocyte regulation ensuring a proper response to infections and cellular stress.

Pathways

The protein LYST is integral in pathways related to vesicular transport such as the endocytic and autophagic pathways. It has a well-documented interaction with proteins such as Rab7 which is important for endosome-to-lysosome trafficking. Additionally LYST interacts with components of the cytoskeleton which further influences lysosome positioning within the cell therefore supporting its central role in cellular logistic pathways.

Mutations or functional disruptions of the LYST protein are linked to Chediak-Higashi Syndrome a rare genetic disorder characterized by immunodeficiency bleeding issues and neurological problems. This condition demonstrates a connection between LYST and the immune response as well as interactions with proteins like Perforin which are also involved in immune functions. Moreover LYST alterations might contribute to pigmentary anomalies in certain skin disorders due to its involvement in melanosome biogenesis.

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Product protocols

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Target data

Adapter protein that regulates and/or fission of intracellular vesicles such as lysosomes (PubMed : 11984006, PubMed : 25216107). Might regulate trafficking of effectors involved in exocytosis (PubMed : 25425525). In cytotoxic T-cells and natural killer (NK) cells, has role in the regulation of size, number and exocytosis of lytic granules (PubMed : 26478006). In macrophages and dendritic cells, regulates phagosome maturation by controlling the conversion of early phagosomal compartments into late phagosomes (By similarity). In macrophages and dendritic cells, specifically involved in TLR3- and TLR4-induced production of pro-inflammatory cytokines by regulating the endosomal TLR3- TICAM1/TRIF and TLR4- TICAM1/TRIF signaling pathways (PubMed : 27881733).

See full target information LYST

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human LYST ELISA Kit

Sandwich ELISA - Human LYST ELISA Kit (AB275905)

Sandwich ELISA - Human LYST ELISA Kit (AB275905)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise