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AB327294

Human M-CSF ELISA Kit, Chemiluminescent

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Human M-CSF ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human M-CSF with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 1.83 - 4000 pg/ml with a sensitivity of 1.09 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
6 Images
Sandwich ELISA - Human M-CSF ELISA Kit, Chemiluminescent (AB327294)
  • sELISA

Supplier Data

Sandwich ELISA - Human M-CSF ELISA Kit, Chemiluminescent (AB327294)

Interpolated concentrations of native M-CSF in human saliva urine and A549 cell supernatant samples. The concentrations of M-CSF were measured in duplicates interpolated from the M-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows : saliva 25% urine 1 : 200 and A549 cell supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean M-CSF concentration was determined to be 0.9 ng/mL in saliva 28.1 ng/mL in urine and 0.89 ng/mL in A549 cell supernatant.

Sandwich ELISA - Human M-CSF ELISA Kit, Chemiluminescent (AB327294)
  • sELISA

Supplier Data

Sandwich ELISA - Human M-CSF ELISA Kit, Chemiluminescent (AB327294)

Serum from eight individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean M-CSF concentration was determined to be 27.8 ng/mL with a range of 17.7 - 47.2 ng/mL.

Sandwich ELISA - Human M-CSF ELISA Kit, Chemiluminescent (AB327294)
  • sELISA

Supplier Data

Sandwich ELISA - Human M-CSF ELISA Kit, Chemiluminescent (AB327294)

Interpolated concentrations of native M-CSF in human serum plasma (heparin) and PHA-M stimulated PBMC cell culture supernatant samples. The concentrations of M-CSF were measured in duplicates interpolated from the M-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2% plasma (heparin) 2.5% and PBMC supernatant 7.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean M-CSF concentration was determined to be 11.5 ng/mL in serum 6.7 ng/mL in plasma (heparin) and 2.4 ng/mL in stimulated PBMC supernatant.

Sandwich ELISA - Human M-CSF ELISA Kit, Chemiluminescent (AB327294)
  • sELISA

Lab

Sandwich ELISA - Human M-CSF ELISA Kit, Chemiluminescent (AB327294)

Standard curve comparison between the Chemiluminescent Human M-CSF SimpleStep ELISA and colorimetric Human M-CSF SimpleStep ELISA (ab245714) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human M-CSF ELISA Kit, Chemiluminescent (AB327294)
  • sELISA

Lab

Sandwich ELISA - Human M-CSF ELISA Kit, Chemiluminescent (AB327294)

Dynamic range comparison between the Chemiluminescent Human M-CSF SimpleStep ELISA, colorimetric Human M-CSF SimpleStep ELISA (ab245714), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human M-CSF ELISA Kit, Chemiluminescent (AB327294)
  • sELISA

Lab

Sandwich ELISA - Human M-CSF ELISA Kit, Chemiluminescent (AB327294)

Interpolated concentration of native M-CSF was measured in duplicate at different sample concentrations. Undiluted samples are 2.5% human plasma citrate and plasma EDTA. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Serum, Urine, Saliva, Heparin Plasma, Citrate plasma, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.09 pg/mL

Range

1.83 - 4000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human M-CSF ELISA Kit, Chemiluminescent (ab327294) is a rapid single-wash sandwich 55-min ELISA to measure Human M-CSF in Cell culture supernatant, Serum, Urine, Saliva, Heparin Plasma, Citrate plasma, EDTA Plasma with a sensitivity of 1.09 pg/mL.

How the assay works

Human M-CSF ELISA Kit, Chemiluminescent (ab327294) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human M-CSF (ab327294) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-human CSF1 antibody [EPR21971-6] - BSA and Aside free (Capture) ab259557
- Anti-human CSF1 antibody [EPR21971-257] - BSA and Aside free (Detector) ab267491
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Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.9" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "87 - 106 %", "average": "= 95" }, { "sample": "Serum", "range": "114 - 122 %", "average": "= 117" }, { "sample": "Urine", "range": "85 - 97 %", "average": "= 92" }, { "sample": "Saliva", "range": "98 - 101 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "107 - 113 %", "average": "= 110" }, { "sample": "Citrate plasma", "range": "76 - 94 %", "average": "= 83" }, { "sample": "EDTA Plasma", "range": "90 - 114 %", "average": "= 98" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
See full target information CSF1

Alternative Names

Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF, CSF1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com