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AB315431

Human MAdCAM1 ELISA Kit- extracellular

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Human MAdCAM1 ELISA Kit- extracellular is a single-wash 90-min Simplestep used to quantify Human MAdCAM1 with a sensitivity of 13 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Mucosal addressin cell adhesion molecule 1, MAdCAM-1, hMAdCAM-1, MADCAM1

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Sandwich ELISA - Human MAdCAM1 ELISA Kit- extracellular (AB315431)
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Supplier Data

Sandwich ELISA - Human MAdCAM1 ELISA Kit- extracellular (AB315431)

Interpolated concentrations of MAdCAM1 in normal human serum donors

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MAdCAM1 concentration was determined to be 52.35 ng/mL with a range of 37.58 – 125.91 ng/mL.

Sandwich ELISA - Human MAdCAM1 ELISA Kit- extracellular (AB315431)
  • sELISA

Supplier Data

Sandwich ELISA - Human MAdCAM1 ELISA Kit- extracellular (AB315431)

Interpolated concentrations of spiked human MAdCAM1 in cell culture media

Recombinant MAdCAM1 was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 50% cell culture media (RPMI 1640 containing 10% fetal bovine serum). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human MAdCAM1 ELISA Kit- extracellular (AB315431)
  • sELISA

Supplier Data

Sandwich ELISA - Human MAdCAM1 ELISA Kit- extracellular (AB315431)

Interpolated concentrations of human MAdCAM1 in serum, plasma (citrate), plasma (EDTA), and plasma (heparin)

Interpolated concentration of native MAdCAM1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 2.5%, plasma (citrate) 2.5%, plasma (EDTA) 2.5%, and plasma (heparin) 2.5%.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human MAdCAM1 ELISA Kit- extracellular (AB315431)
  • sELISA

Supplier Data

Sandwich ELISA - Human MAdCAM1 ELISA Kit- extracellular (AB315431)

Example of human MAdCAM1 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 13 pg/mL

Range

39.063 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB307734

Anti-MAdCAM1 antibody [EPR27555-87]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAdCAM1 antibody [EPR27555-87] (AB307734)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human MAdCAM1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MAdCAM1 protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Cell culture media. Quantitate Human MAdCAM1 with 13 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "-1.7" } ]
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Recovery

[ { "sample": "Serum", "range": "92 - 100 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "99 - 119 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "87 - 91 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "89 - 115 %", "average": "= 100" }, { "sample": "Cell culture media", "range": "80 - 109 %", "average": "= 101" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAdCAM-1 also known as mucosal addressin cell adhesion molecule 1 or mucosal tag is a protein with a molecular mass of approximately 60 kDa. The MAdCAM-1 protein is expressed prominently on high endothelial venules within mucosal tissues such as the gut and the colon. Additionally it also appears in the endothelial cells of lymphoid tissues. Another name for it is MECA-79 highlighting its recognition by the MECA-79 antibody. Mucosal tags label regions where MAdCAM-1 assists in cell adhesion processes.
Biological function summary

MAdCAM-1 facilitates lymphocyte homing to mucosal tissues. It plays a part in the adhesive interactions between endothelial cells and lymphocytes as a component of the immune surveillance system enabling T cells and B cells to move into areas where they are needed. MAdCAM-1 forms complexes with integrins notably alpha4beta7 which are integral to its adhesion functions. Through its role in guiding lymphocytes it influences immune response and inflammation in mucosal environments.

Pathways

MAdCAM-1 contributes importantly to the trafficking of immune cells. It is involved in the regulation of cell adhesion and migration pathways. The protein interacts extensively with the integrin family especially integrin alpha4beta7 which facilitates lymphocyte entry into the intestinal wall. Additionally it works with chemokine receptors and cytokines that further influence cell movement and homing to specific tissues. This integrative role reinforces its importance in immune response and mucosal immunity.

MAdCAM-1 links closely to inflammatory diseases such as Crohn's disease and ulcerative colitis. Its expression is upregulated in these inflammatory conditions which leads to increased lymphocyte accumulation in the gut. This condition exacerbates the inflammation and tissue damage characteristic of these disorders. MECA-79 a monoclonal antibody targets MAdCAM-1 to aid therapeutic approaches in mitigating inflammation driven by lymphocyte infiltration. Understanding MAdCAM-1 and its function offers potential pathways to target and treat these chronic inflammatory diseases.
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Product protocols

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Target data

Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
See full target information MADCAM1
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websiteProtocolBooklet
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Product promise

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