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AB303742

Human MALT1/MLT ELISA Kit

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Human MALT1/MLT ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MALT1/MLT with a sensitivity of 27.02 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

MLT, MALT1, Mucosa-associated lymphoid tissue lymphoma translocation protein 1, MALT lymphoma-associated translocation, Paracaspase

2 Images
Sandwich ELISA - Human MALT1/MLT ELISA Kit (AB303742)
  • sELISA

Supplier Data

Sandwich ELISA - Human MALT1/MLT ELISA Kit (AB303742)

Example of human MALT1/MLT standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human MALT1/MLT ELISA Kit (AB303742)
  • sELISA

Supplier Data

Sandwich ELISA - Human MALT1/MLT ELISA Kit (AB303742)

Interpolated concentrations of human MALT1/MLT in Jurkat extract and human tonsil extract.

Interpolated concentration of native MALT1/MLT. was measured in duplicate at different sample concentrations. Undiluted samples are 500 µg/mL Jurkat and human tonsil extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 27.02 pg/mL

Range

187.5 - 12000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB33921

Anti-MALT1/MLT antibody [EP603Y]

KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-MALT1/MLT antibody [EP603Y] (AB33921)

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Proteins & Peptides

AB271604

Recombinant human MALT1/MLT protein (Active) (DDDDK tag N-Terminus)

Functional Studies - Recombinant human MALT1/MLT protein (Active) (DDDDK tag N-Terminus) (AB271604)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human MALT1/MLT SimpleStep ELISA® kit (ab303742) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human MALT1/MLT protein in cell lysate and tissue extract. Quantitate Human MALT1/MLT with 27.02 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.4" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": null, "average": "= 90" }, { "sample": "Tissue Extracts", "range": null, "average": "= 82" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab303742-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB303742-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB303742-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB303742-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB303742-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS", "number":"AB303742-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB303742-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB303742-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human MALT1/MLT Lyophilized Recombinant Protein", "number":"AB303742-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MALT1/MLT Capture Antibody", "number":"AB303742-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR", "number":"AB303742-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MALT1/MLT Detector Antibody", "number":"AB303742-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Protease that enhances BCL10-induced activation : acts via formation of CBM complexes that channel adaptive and innate immune signaling downstream of CARD domain-containing proteins (CARD9, CARD11 and CARD14) to activate NF-kappa-B and MAP kinase p38 pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (PubMed : 11262391, PubMed : 18264101, PubMed : 24074955). Mediates BCL10 cleavage : MALT1-dependent BCL10 cleavage plays an important role in T-cell antigen receptor-induced integrin adhesion (PubMed : 11262391, PubMed : 18264101). Involved in the induction of T helper 17 cells (Th17) differentiation (PubMed : 11262391, PubMed : 18264101). Cleaves RC3H1 and ZC3H12A in response to T-cell receptor (TCR) stimulation which releases their cooperatively repressed targets to promote Th17 cell differentiation (By similarity). Also mediates cleavage of N4BP1 in T-cells following TCR-mediated activation, leading to N4BP1 inactivation (PubMed : 31133753). May also have ubiquitin ligase activity : binds to TRAF6, inducing TRAF6 oligomerization and activation of its ligase activity (PubMed : 14695475).
See full target information MALT1
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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