AB277420

Human Mannose Receptor ELISA kit

Name: Human Mannose Receptor ELISA kit (ab277420) | Abcam
Brand: Abcam Limited
SKU: AB277420

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(2 Publications)

Human Mannose Receptor ELISA kit is a Sandwich (quantitative) ELISA for the measurement of Human Mannose Receptor in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD206, CLEC13D, CLEC13DL, MRC1L1, MRC1, Macrophage mannose receptor 1, MMR, C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like, Human mannose receptor, Macrophage mannose receptor 1-like protein 1, hMR

1 Images

ELISA - Human Mannose Receptor ELISA kit (AB277420)

ELISA

Supplier Data

ELISA - Human Mannose Receptor ELISA kit (AB277420)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.41 ng/mL

Range

0.41 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Mannose Receptor ELISA Kit (ab277420) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Mannose Receptor in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human Mannose Receptor coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Mannose Receptor present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Mannose Receptor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Mannose Receptor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]

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Recovery

[ { "sample": "Serum", "range": "83 - 131 %", "average": "= 104.6" }, { "sample": "Plasma", "range": "93 - 142 %", "average": "= 110.3" }, { "sample": "Cell culture media", "range": "91 - 127 %", "average": "= 109.3" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277420-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277420-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277420-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Mannose Receptor coated Microplate", "number":"AB277420-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB277420-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB277420-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277420-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277420-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Mannose Receptor standard protein (Lyophilized)", "number":"AB277420-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Mannose Receptor Antibody", "number":"AB277420-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Mannose Receptor also known as CD206 or MRC1 is a type of C-type lectin receptor with a molecular weight of 175 kDa. It is expressed on the surface of macrophages and dendritic cells. This receptor binds to complex carbohydrates like mannose and fucose found on pathogen surfaces playing an important role in their recognition. Mannose Receptors act as endocytic receptors aiding in the internalization and degradation of pathogens.

Biological function summary

The Mannose Receptor functions in pathogen recognition and clearance by binding to specific carbohydrates on the pathogen's surface. It is not part of a larger molecular complex but works independently to facilitate phagocytosis. The receptor is essential in immune responses allowing macrophages and dendritic cells to efficiently capture and process antigens for presentation to T cells. This enables the initiation of adaptive immune responses.

Pathways

Researchers know that the Mannose Receptor plays an important role in immune signaling pathways particularly in antigen processing and presentation. It interacts with proteins involved in these pathways including those necessary for the internalization and degradation of bound antigens. Moreover it works alongside scavenger receptors contributing to the regulation of immune and inflammatory responses.

The Mannose Receptor is often linked to pulmonary tuberculosis and certain types of cancer. When macrophages express this receptor it can modulate immune responses against Mycobacterium tuberculosis by aiding in its recognition. In cancer altered expression of CD206 can indicate tumor-associated macrophages which promote tumor growth by suppressing immune responses. These connections highlight the receptor's potential as a diagnostic marker and therapeutic target.

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Product protocols

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Target data

Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains.. (Microbial infection) Acts as a phagocytic receptor for bacteria, fungi and other pathogens.. (Microbial infection) Acts as a receptor for Dengue virus envelope protein E.. (Microbial infection) Interacts with Hepatitis B virus envelope protein.

See full target information MRC1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:2057 PubMed37045813

2023

Targeting neuronal lysosomal dysfunction caused by β-glucocerebrosidase deficiency with an enzyme-based brain shuttle construct.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra Gehrlein,Vinod Udayar,Nadia Anastasi,Martino L Morella,Iris Ruf,Doris Brugger,Sophia von der Mark,Ralf Thoma,Arne Rufer,Dominik Heer,Nina Pfahler,Anton Jochner,Jens Niewoehner,Luise Wolf,Matthias Fueth,Martin Ebeling,Roberto Villaseñor,Yanping Zhu,Matthew C Deen,Xiaoyang Shan,Zahra Ehsaei,Verdon Taylor,Ellen Sidransky,David J Vocadlo,Per-Ola Freskgård,Ravi Jagasia

Experimental and therapeutic medicine 25:169 PubMed37006880

2023

IGFBP‑rP1 affects the proliferation, apoptosis and macrophage polarization of endometrial cancer cells by regulating the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Gao,Shiqi Suo,Jingxia Li,Chunxia Wang,Ranran Deng,Yuxia Hu,Chunzheng Zhang

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Mannose Receptor ELISA kit

ELISA - Human Mannose Receptor ELISA kit (AB277420)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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