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AB234562

Human MAPK13 ELISA Kit

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Human MAPK13 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MAPK13 with a sensitivity of 38.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

PRKM13, SAPK4, MAPK13, Mitogen-activated protein kinase 13, MAP kinase 13, MAPK 13, Mitogen-activated protein kinase p38 delta, Stress-activated protein kinase 4, MAP kinase p38 delta

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Sandwich ELISA - Human MAPK13 ELISA Kit (AB234562)
  • sELISA

Supplier Data

Sandwich ELISA - Human MAPK13 ELISA Kit (AB234562)

Interpolated concentrations of native MAPK13 in human HEK293T and A431 cell extract samples based on a 500 μg/mL extract load.

The concentrations of MAPK13 were measured in duplicate and interpolated from the MAPK13 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MAPK13 concentration was determined to be 9009 pg/mL in HEK293T and 8141 pg/mL in A431 cell extract samples.

Sandwich ELISA - Human MAPK13 ELISA Kit (AB234562)
  • sELISA

Supplier Data

Sandwich ELISA - Human MAPK13 ELISA Kit (AB234562)

Example of human MAPK13 standard curve in 1X Cell Extraction Buffer PTR.

The MAPK13 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human MAPK13 ELISA Kit (AB234562)
  • sELISA

Supplier Data

Sandwich ELISA - Human MAPK13 ELISA Kit (AB234562)

Representative image of Human MAPK13 ELISA Kit (ab234562)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human MAPK13 Capture Antibody

- 10X Human MAPK13 Detector Antibody

- Human MAPK13 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent BAR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 38.4 pg/mL

Range

141 - 9000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

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p38 MAPK alpha ELISA Kit

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Primary Antibodies

AB245020

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Primary Antibodies

AB178867

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Proteins & Peptides

AB271718

Recombinant Human PRMT2/HMT1 protein (DDDDK tag N-Terminus)

SDS-PAGE - Recombinant Human PRMT2/HMT1 protein (DDDDK tag N-Terminus) (AB271718)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human MAPK13 ELISA Kit (ab234562) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MAPK13 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MAPK13 with 38.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "117 - 124 %", "average": "= 119" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab234562-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB234562-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB234562-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB234562-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB234562-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB234562-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB234562-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB234562-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human MAPK13 Lyophilized Recombinant Protein", "number":"AB234562-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent BAR", "number":"AB234562-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MAPK13 Capture Antibody", "number":"AB234562-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MAPK13 Detector Antibody", "number":"AB234562-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB234562-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P38 delta also known as MAPK13 is a member of the mitogen-activated protein kinase (MAPK) family. It has a molecular weight of about 43 kDa. This kinase is involved in transmitting signals from the cell surface to the DNA in the cell nucleus affecting gene expression. You can find MAPK13 expression in various tissues such as skin lung and pancreas. It plays a role in cellular responses to stress and inflammation helping to regulate diverse cellular processes like gene expression differentiation and apoptosis.
Biological function summary

The function of MAPK13 extends to its involvement in multiple cellular processes linked to stress responses. It forms part of a signaling complex that responds to extracellular stimuli. MAPK13 contributes to regulating inflammation by mediating the transcriptional activity of specific genes. Through phosphorylation it modifies other proteins and influences pathways that control cell growth and apoptosis. Cellular stress and environmental changes activate this kinase highlighting its role in helping cells adapt.

Pathways

MAPK13 integrates into the MAPK signaling pathway contributing to the transmission and amplification of signals related to cellular stress and inflammatory responses. It interacts with other proteins like MAPK14 (p38 alpha) within these pathways emphasizing its role in mediating cellular reactions to stress. The MAPK pathway influences the activity of transcription factors impacting gene expression and cellular outcomes. MAPK13 also plays a role in the p38 MAPK pathway emphasizing its connection within these essential signaling networks.

MAPK13 relates to conditions such as inflammatory diseases and cancer. Its role in inflammation links MAPK13 to diseases like chronic obstructive pulmonary disease (COPD) where excessive inflammatory responses contribute to disease progression. It also connects to cancer progression where abnormal cell signaling leads to uncontrolled growth. In these conditions MAPK13 interacts with other proteins such as NF-kB which further influence disease outcomes by modulating inflammatory and cell survival pathways.
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Product protocols

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Target data

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as pro-inflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK13 is one of the less studied p38 MAPK isoforms. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K. Involved in cytoskeletal remodeling through phosphorylation of MAPT and STMN1. Mediates UV irradiation induced up-regulation of the gene expression of CXCL14. Plays an important role in the regulation of epidermal keratinocyte differentiation, apoptosis and skin tumor development. Phosphorylates the transcriptional activator MYB in response to stress which leads to rapid MYB degradation via a proteasome-dependent pathway. MAPK13 also phosphorylates and down-regulates PRKD1 during regulation of insulin secretion in pancreatic beta cells.
See full target information MAPK13
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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