Human MASP2 ELISA Kit
- Recombinant
- SimpleStep
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Human MASP2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MASP2 with a sensitivity of 21 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Mannan-binding lectin serine protease 2, MBL-associated serine protease 2, Mannose-binding protein-associated serine protease 2, MASP-2, MASP2
- ELISA
Supplier Data
ELISA - Human MASP2 ELISA Kit (AB278121)
- sELISA
Supplier Data
Sandwich ELISA - Human MASP2 ELISA Kit (AB278121)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MASP2 concentration was determined to be 343.24 ng/mL with a range of 56.31 – 528.11 ng/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human MASP2 ELISA Kit (AB278121)
Interpolated concentrations of native MASP2 in human serum and plasma samples.
The concentrations of MASP2 were measured in duplicates, interpolated from the MASP2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1%, plasma (citrate) 1%, plasma (heparin) 1% and plasma (EDTA) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MASP2 concentration was determined to be 484.87 ng/mL in serum, 561.89 ng/mL in plasma (citrate), 500.49 ng/mL in plasma (heparin), and 404.43 ng/mL in plasma (EDTA).
- sELISA
Supplier Data
Sandwich ELISA - Human MASP2 ELISA Kit (AB278121)
Example of human MASP2 standard curve in Sample Diluent NS.
The MASP2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Complementary Products
Reactivity data
Product details
Human MASP2 ELISA Kit ab278121 is a rapid single-wash 90-min Sandwich ELISA to measure Human MASP2 in EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 21 pg/mL.
How the assay works
Human MASP2 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human MASP2 ELISA Kit ab278121 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-MASP2 antibody [EPR23588-1] - BSA and Azide free (Capture) ab281543
- Anti-MASP2 antibody [EPR23588-65] - BSA and Azide free (Detector) ab281543
MASP2, Mannan-binding lectin serine protease 2, plays a key role in the complement activation cascade. This protein is part of a larger mannose-binding complex which senses foreign material, activates C3 convertase, and perpetuates the immune response. The immunogen from which the antibodies in this kit were raised represents the N-terminal half of the protein.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MASP-2 associates with MBL (Mannose-binding lectin) and forms a complex critical for the lectin pathway's function. In this complex MASP-2 initiates the cleavage of complement proteins C4 and C2 forming the C3 convertase. This action enhances the immune response by promoting the clearance of pathogens. MASP-1 is another protease that works closely with MASP-2 in this pathway although the two have distinct roles—MASP-2 is primarily responsible for the activation of the downstream components of the cascade.
Pathways
MASP-2 plays an integral role in the complement system and particularly in the lectin pathway which is one of the three pathways of complement activation. It connects with both the classical and alternative pathways through the formation of C3 convertase a critical step in the complement cascade. The interactions with other proteins like MASP-1 and MBL are significant; MASP-1 has an additional role in the activation sequence by further augmenting MASP-2's activity and facilitating efficient pathogen clearance.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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