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AB325738

Human MASP2 ELISA Kit, Chemiluminescent

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Human MASP2 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human MASP2 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.03 - 60 ng/ml with a sensitivity of 0.01ng/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

Mannan-binding lectin serine protease 2, MBL-associated serine protease 2, Mannose-binding protein-associated serine protease 2, MASP-2, MASP2

5 Images
Sandwich ELISA - Human MASP2 ELISA Kit, Chemiluminescent (AB325738)
  • sELISA

Lab

Sandwich ELISA - Human MASP2 ELISA Kit, Chemiluminescent (AB325738)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human MASP2 ELISA Kit, Chemiluminescent (AB325738)
  • sELISA

Lab

Sandwich ELISA - Human MASP2 ELISA Kit, Chemiluminescent (AB325738)

Dynamic range comparison between the Chemiluminescent Human MASP2 SimpleStep ELISA, colorimetric Human MASP2 SimpleStep ELISA (ab278121), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased dynamic range.

Sandwich ELISA - Human MASP2 ELISA Kit, Chemiluminescent (AB325738)
  • sELISA

Lab

Sandwich ELISA - Human MASP2 ELISA Kit, Chemiluminescent (AB325738)

Standard curve comparison between the Chemiluminescent Human MASP2 SimpleStep ELISA and colorimetric Human MASP2 SimpleStep ELISA (ab278121) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human MASP2 ELISA Kit, Chemiluminescent (AB325738)
  • sELISA

Supplier Data

Sandwich ELISA - Human MASP2 ELISA Kit, Chemiluminescent (AB325738)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean MASP2 concentration was determined to be 343.24 ng/mL with a range of 56.31 – 528.11 ng/mL.

Sandwich ELISA - Human MASP2 ELISA Kit, Chemiluminescent (AB325738)
  • sELISA

Supplier Data

Sandwich ELISA - Human MASP2 ELISA Kit, Chemiluminescent (AB325738)

Interpolated concentrations of native MASP2 in human serum and plasma samples.

The concentrations of MASP2 were measured in duplicates interpolated from the MASP2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1% plasma (citrate) 1% plasma (heparin) 1% and plasma (EDTA) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean MASP2 concentration was determined to be 484.87 ng/mL in serum 561.89 ng/mL in plasma (citrate) 500.49 ng/mL in plasma (heparin) and 404.43 ng/mL in plasma (EDTA).

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Heparin Plasma, Citrate plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.01 ng/mL

Range

0.03 - 60 ng/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human MASP2 ELISA Kit, Chemiluminescent (ab325738) is a rapid single-wash sandwich 55-min ELISA to measure Human MASP2 in Serum, EDTA Plasma, Heparin Plasma, Citrate plasma with a sensitivity of 0.01 ng/mL.

How the assay works

Human MASP2 ELISA Kit, Chemiluminescent (ab325738) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human MASP2 (ab325738) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-MASP2 antibody [EPR23588-1] - BSA and Aside free (Capture) ab281519
- Anti-MASP2 antibody [EPR23588-65] - BSA and Aside free (Detector) ab281543
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.7" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.4" } ]
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Recovery

[ { "sample": "Serum", "range": "87 - 111 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "86 - 106 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "97 - 114 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "80 - 103 %", "average": "= 91" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325738-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human MASP2 Capture Antibody", "number":"AB325738-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human MASP2 Lyophilized Recombinant Protein", "number":"AB325738-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325738-CMP10", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325738-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325738-CMP06", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325738-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325738-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB325738-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325738-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MASP2 Detector Antibody", "number":"AB325738-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Precursor of a serum protease that activates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 11527969, PubMed : 22691502). The lectin complement system is activated following association of lectins, such as MBL2, FCN1, FCN2 or FCN3, to carbohydrates on the pathogen surface (PubMed : 22691502, PubMed : 22966085). MASP2 is cleaved and activated by MASP1 in response to lectin-binding to pathogen carbohydrates (PubMed : 10946292, PubMed : 22949645, PubMed : 22966085, PubMed : 9087411). Can activate prothrombin to thrombin (PubMed : 39924859).. Mannan-binding lectin serine protease 2 B chain. Serine protease component of the lectin complement pathway, which catalyzes cleavage and activation of C2 and C4, the next components of the complement pathway.
See full target information MASP2
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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