AB218269

Human MASPIN ELISA Kit

Recombinant
SimpleStep
What is this?

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(1 Publication)

Human MASPIN ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MASPIN with a sensitivity of 8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

PI5, SERPINB5, Serpin B5, Maspin, Peptidase inhibitor 5, PI-5

4 Images

Sandwich ELISA - Human MASPIN ELISA Kit (AB218269)

sELISA

Supplier Data

Sandwich ELISA - Human MASPIN ELISA Kit (AB218269)

Interpolated concentrations of native MASPIN in human PC-3, A431, HL-60, and Hela cell extract based on a 10 μg/mL extract load.

The concentrations of MASPIN were measured in duplicate, interpolated from the MASPIN standard curve. The interpolated, dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean MASPIN concentration was determined to be 55 ng/mL, 93 ng/mL, and non-detectable in PC-3, A431, HL-60, and HeLa cell extract, respectively.

sELISA

Supplier Data

Sandwich ELISA - Human MASPIN ELISA Kit (AB218269)

Interpolated concentrations of native MASPIN in human PC-3 cell extract based on a 1,000 μg/mL extract load.

The concentrations of MASPIN were measured in duplicate, interpolated from the MASPIN standard curve, and corrected for sample dilution. The interpolated, dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean MASPIN concentration was determined to be 57.5 ng/mL in PC-3 cell extract.

sELISA

Supplier Data

Sandwich ELISA - Human MASPIN ELISA Kit (AB218269)

Representative image of Human MASPIN ELISA Kit (ab218269)

Components shown from left to right :

- Sample diluent NS

- 10X Wash Buffer PT (ab206977)

- 10X Human MASPIN Detector Antibody

- Human MASPIN Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Human MASPIN Capture Antibody (Lyophilised)

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Human MASPIN ELISA Kit (AB218269)

Example of human MASPIN standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 8 pg/mL

Range

0.11 - 7 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human MASPIN ELISA Kit (ab218269) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MASPIN protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MASPIN with 8 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

MASPIN (Mammary Serine Protease Inhibitor) is a 375 amino acid glycosylated protein encoded by the gene SERPINB5. It belongs to a family of proteins known as serpins (serine protease inhibitor), which function as regulators of protein breakdown by acting as suicide inhibitors of the catalytic activity of proteinases. Inhibitory serpins undergo a transition between a stressed and relaxed confirmation, where the stressed confirmation binds to the catalytic serine residue of the protease. MASPIN is thought to interact with extracellular matrix components, glutathione S-transferase, and interferon regulatory factor 6. It was originally thought to play a role in tumor metastasis, but the exact function is unknown.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.4" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" } ]

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Recovery

[ { "sample": "Cell culture extracts", "range": "107 - 109 %", "average": "= 108" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab218269-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB218269-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB218269-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB218269-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB218269-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB218269-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB218269-CMP11", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human MASPIN Capture Antibody (Lyophilized)", "number":"AB218269-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB218269-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human MASPIN Lyophilized Recombinant Protein", "number":"AB218269-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB218269-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB218269-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MASPIN Detector Antibody", "number":"AB218269-CMP10", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MASPIN also known as SERPINB5 is a non-inhibitory serine protease inhibitor with a mass of approximately 42 kDa. It functions mechanically to regulate cell adhesion migration apoptosis and angiogenesis. MASPIN localizes mainly in the cytoplasm and nucleus and expresses predominantly in epithelial cells including those in the mammary gland prostate and keratinocytes in the skin. It acts as a tumor suppressor and researchers often use immunohistochemistry (IHC) to study its expression patterns in various tissues.

Biological function summary

MASPIN influences apoptosis and cell motility playing a critical role in tumor suppression. It does not form part of a complex but interacts directly with extracellular matrix components. MASPIN can inhibit angiogenesis by reducing the proliferation and migration of endothelial cells. Scientists have noted its role in maintaining normal cell architecture and inhibiting tumor cell invasion.

Pathways

Researchers place MASPIN in important signaling networks impacting cancer progression and metastasis. It participates in the PI3K/AKT pathway thereby influencing cell growth and survival. MASPIN also intersects with TGF-β signaling and related proteins like SMAD which mediate growth arrest and apoptosis. By altering these pathways MASPIN helps modulate responses to cellular stress and inhibit lethal growth in cancer cells.

MASPIN often associates with breast and prostate cancers. Its expression inversely correlates with tumor progression suggesting a protective role. MASPIN's interaction with proteins like p53 highlights its contribution to tumor suppression. Abnormal MASPIN presence links to aggressive disease phenotypes making it a potential biomarker for prognostic evaluation in oncology.

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Product protocols

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Target data

Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

See full target information SERPINB5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 9:10731 PubMed31341177

2019

Differential responses of epithelial cells from urinary and biliary tract to eggs of Schistosoma haematobium and S. mansoni.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael Nacif-Pimenta,Alessandra da Silva Orfanó,Ilana A Mosley,Shannon E Karinshak,Kenji Ishida,Victoria H Mann,Paulo Marcos Zech Coelho,José M Correia da Costa,Michael H Hsieh,Paul J Brindley,Gabriel Rinaldi

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human MASPIN ELISA Kit

Sandwich ELISA - Human MASPIN ELISA Kit (AB218269)

Sandwich ELISA - Human MASPIN ELISA Kit (AB218269)

Sandwich ELISA - Human MASPIN ELISA Kit (AB218269)

Sandwich ELISA - Human MASPIN ELISA Kit (AB218269)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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